Low‐Voltage Pulsed Electric Field Sterilization on a Microfluidic Chip

Liu, Linlin; Zhao, Liping; Yang, Jun; Wan, Xiaoping; Hu, Ning; Yeh, Li‐Hsien; Joo, Sang Woo; Qian, Shizhi

doi:10.1002/elan.201200648

Cited by 8 publications

(4 citation statements)

References 41 publications

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“…A microchip with coplanar electrode arrays of symmetrical comb teeth shown in Figure 1 b was designed and fabricated based on the flexible printed circuit board (FPCB) technique following reference [ 17 ]. The raised comb teeth in the polyimide substrate-based microchip formed a subcell for GUV formation on one hand, and strengthened the electric field on the other hand.…”

Section: Methodsmentioning

confidence: 99%

Frequency-Dependent Electroformation of Giant Unilamellar Vesicles in 3D and 2D Microelectrode Systems

et al. 2017

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A giant unilamellar vesicle (GUV), with similar properties to cellular membrane, has been widely studied. Electroformation with its simplicity and accessibility has become the most common method for GUV production. In this work, GUV electroformation in devices with traditional 3D and new 2D electrode structures were studied with respect to the applied electric field. An optimal frequency (10 kHz in the 3D and 1 kHz in the 2D systems) was found in each system. A positive correlation was found between GUV formation and applied voltage in the 3D electrode system from 1 to 10 V. In the 2D electrode system, the yield of the generated GUV increased first but decreased later as voltage increased. These phenomena were further confirmed by numerically calculating the load that the lipid film experienced from the generated electroosmotic flow (EOF). The discrepancy between the experimental and numerical results of the 3D electrode system may be because the parameters that were adopted in the simulations are quite different from those of the lipid film in experiments. The lipid film was not involved in the simulation of the 2D system, and the numerical results matched well with the experiments.

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Section: Methodsmentioning

confidence: 99%

Frequency-Dependent Electroformation of Giant Unilamellar Vesicles in 3D and 2D Microelectrode Systems

et al. 2017

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“…It takes 3–5 min to align a single cell, parallelization is challenging, and optical forces may not be sufficiently strong to easily move particularly sticky cells . Instead, DEP forces have been used frequently to position cells in microscale EP devices. ,,,,,− Unlike optical tweezers, DEP forces are effective for large numbers of cells in parallel. DEP forces are exerted on polarizable particles using a nonuniform electric field to manipulate cells at the microscale and have been widely used for microfluidic cell sorting. , The earliest example of DEP to assist microscale EP used triangular electrode arrays to draw cells toward sharp-edged electrodes in a microfluidic channel for yeast, bacteria, plant, and mammalian cell lysis at low voltages. , Kim et al used DEP forces to guide bacteria into a microwell array with single-cell trapping. − The DEP forces were stronger than bacteria motility to restrict cells from swimming out of the microwells.…”

Section: Technological Improvementsmentioning

confidence: 99%

Recent Advances in Microscale Electroporation

2022

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Electroporation (EP) is a commonly used strategy to increase cell permeability for intracellular cargo delivery or irreversible cell membrane disruption using electric fields. In recent years, EP performance has been improved by shrinking electrodes and device structures to the microscale. Integration with microfluidics has led to the design of devices performing static EP, where cells are fixed in a defined region, or continuous EP, where cells constantly pass through the device. Each device type performs superior to conventional, macroscale EP devices while providing additional advantages in precision manipulation (static EP) and increased throughput (continuous EP). Microscale EP is gentle on cells and has enabled more sensitive assaying of cells with novel applications. In this Review, we present the physical principles of microscale EP devices and examine design trends in recent years. In addition, we discuss the use of reversible and irreversible EP in the development of therapeutics and analysis of intracellular contents, among other noteworthy applications. This Review aims to inform and encourage scientists and engineers to expand the use of efficient and versatile microscale EP technologies.

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“…The configuration of the microelectrode arrays influenced the bacterial sterilization. Firstly, the bacteria were concentrated in the high electric field region by dielectrophoresis and then the enriched bacteria were killed by the pulsed electric field through microelectrode arrays [ 77 ].…”

Section: Microfluidics Devices Accompanied With Supporting Technolmentioning

confidence: 99%

The Role of Microfluidics for Organ on Chip Simulations

Aziz

Geng

et al. 2017

Bioengineering

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A multichannel three-dimensional chip of a microfluidic cell culture which enables the simulation of organs is called an “organ on a chip” (OC). With the integration of many other technologies, OCs have been mimicking organs, substituting animal models, and diminishing the time and cost of experiments which is better than the preceding conventional in vitro models, which make them imperative tools for finding functional properties, pathological states, and developmental studies of organs. In this review, recent progress regarding microfluidic devices and their applications in cell cultures is discussed to explain the advantages and limitations of these systems. Microfluidics is not a solution but only an approach to create a controlled environment, however, other supporting technologies are needed, depending upon what is intended to be achieved. Microfluidic platforms can be integrated with additional technologies to enhance the organ on chip simulations. Besides, new directions and areas are mentioned for interested researchers in this field, and future challenges regarding the simulation of OCs are also discussed, which will make microfluidics more accurate and beneficial for biological applications.

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Low‐Voltage Pulsed Electric Field Sterilization on a Microfluidic Chip

Cited by 8 publications

References 41 publications

Frequency-Dependent Electroformation of Giant Unilamellar Vesicles in 3D and 2D Microelectrode Systems

Frequency-Dependent Electroformation of Giant Unilamellar Vesicles in 3D and 2D Microelectrode Systems

Recent Advances in Microscale Electroporation

The Role of Microfluidics for Organ on Chip Simulations

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