Tissue culture parameters in sweet orange cultivars

Mendes, Amanda Ferreira da Silva; Cidade, Luciana C.; Manzoli, Gabrielle Novais; Otoni, Wagner Campos; Filho, Walter dos Santos Soares; Costa, Márcio Gilberto Cardoso

doi:10.1590/s0100-204x2008000800020

Cited by 6 publications

(9 citation statements)

References 10 publications

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“…The present work corroborates these findings and further demonstrates that the optimal hormone concentrations are specific to cultivar, as it has been previously reported for juvenile explants (Bordón et al, 2000;Costa et al, 2004;Mendes et al, 2008).…”

Section: Resultssupporting

confidence: 93%

Growth regulators, culture media and antibiotics in the in vitro shoot regeneration from mature tissue of citrus cultivars

Oliveira

Costa

Silva

et al. 2010

Pesq. agropec. bras.

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-The objective of this study was to evaluate the effects of 6-benzylaminopurine (BAP) and α-naphthaleneacetic acid (NAA) combinations, basal media and beta-lactam antibiotics on in vitro organogenesis from mature stem segments of 'Pêra', 'Valência' and 'Bahia' sweet oranges and 'Cravo' rangpur lime. For induction of shoot regeneration, the segments of the four cultivars were placed on Murashige and Reguladores de crescimento, meio de cultura e antibióticos na regeneração in vitro de tecidos maduros de cultivares de citrosResumo -O objetivo deste estudo foi avaliar o efeito de combinações de 6-benzilaminopurina (BAP) e ácido α-naftalenoacético (NAA), meios de cultura e antibióticos beta-lactâmicos sobre a regeneração in vitro de segmentos internodais de laranja doce ('Pêra', 'Valência' e 'Bahia') e limão 'Cravo'. Para a indução de brotações, os explantes internodais foram cultivados em meio Murashige e Skoog ( . A fim de testar a influência dos meios de cultura na organogênese, as formulações (MS), Murashige e Tucker (MT) e "woody plant medium" (WPM) foram avaliadas, associadas à melhor combinação de reguladores de crescimento obtida no experimento anterior. Foram utilizados quatro antibióticos beta-lactâmicos (timentin, cefotaxima, trihidrato de meropenem e augmentin), em diferentes concentrações, para avaliar sua influência na regeneração in vitro. As melhores respostas foram obtidas quando os explantes foram cultivados em meio MS com 500 mg L -1 de cefotaxima com as seguintes combinações BAP/ANA: 0,5 + 0,25 mg L -1 , para 'Cravo'; 1,0 + 0,25 mg L -1 , para 'Valência' e 'Bahia'; e 1,0 + 0,5 mg L -1

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Section: Resultssupporting

confidence: 93%

Growth regulators, culture media and antibiotics in the in vitro shoot regeneration from mature tissue of citrus cultivars

Oliveira

Costa

Silva

et al. 2010

Pesq. agropec. bras.

Self Cite

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“…), becoming the system inefficient and more complicated due to the need of micrografting. To our knowledge, only a few systematic studies evaluated the possible factors affecting in vitro rhizogenesis in citrus, which included auxins (Moreira-Dias et al 2000, Mendes et al 2008, temperature and photosynthetic radiation (Duran-Vila et al 1992). The role of polyamines (PAs) in this process has not been investigated, despite their relevance acquired during the last decade in relation to rhizogenesis (Couée et al 2004).…”

Section: ⎯⎯⎯⎯mentioning

confidence: 99%

Role of auxins, polyamines and ethylene in root formation and growth in sweet orange

Mendes¹,

Cidade²,

Otoni³

et al. 2011

Biol. plant.

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The primary objective of this work was to investigate the role of polyamines (PAs) on root formation and growth in two sweet orange (Citrus sinensis L. Osb.) cultivars Pineapple and Pêra. Adventitious shoots (30-d-old) derived from epicotyl explants were transferred to root induction medium containing Murashige and Skoog salts at different strengths and supplemented with different concentrations and combinations of auxins. Root formation and development decreased in both sweet orange cultivars concomitant with the reduction of medium strength. The α-naphtaleneacetic acid was important during the root differentiation phase, but its combination with indole-3-butyric acid was essential for root elongation. The addition of PAs significantly improved root formation and/or growth, depending on their concentration, whereas the presence of inhibitor of PAs biosynthesis α-difluoromethylornithine (DFMO) inhibited these processes. The rooting impairment caused by DFMO was partially reversed by the supplementation of putrescine. Aminoethoxyvinylglycine AVG and AgNO 3 also inhibited in vitro rooting in both sweet orange cultivars, indicating that ethylene was likewise important for rhizogenesis in sweet orange.

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“…Since the requirement of BAP for shoot development was genotype specific [9], for mandarin orange cv. 'Manakamana Local' to produce in-vitro scion for micro-grafting the MS basal media+0.20mg/l IAA in immature shoot is …”

Section: Resultsmentioning

confidence: 99%

“…Similarly, highest shooting directly from explants ( Figure 1) (31.195%) was observed from in-vitro epicotyle ( Table 2). Mendes et al [9] also observed that epicotyl explants from 35-day-old seedlings produced significantly more shoots per explants in sweet orange variety 'Para'. No shooting from callus was observed on MS basal media+BAP 4 mg/l with/without IAA.…”

Section: Shootingmentioning

confidence: 90%

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Hormonal Effect on Mandarin Orange (Citrus reticulata Blanco) Micro-propagation

2016

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Tissue culture is the best option to produce disease free seedling of the fruit crop rapidly. Micro-propagation and use of the in-vitro grafting (micro-grafting) is very helpful for production of virus free planting materials in mandarin. Different levels of the in-vitro hormone affect the success of callusing, shooting and plant regeneration in mandarin. Shoot bud, flower bud and in-vitro seedling epicotyl was used as explants to study the hormonal effect on mandarin micro-propagation. Similarly, 10 levels of BAP and IAA combination on MS media for mandarin tissue culture were used. Observation was done for 100 test tubes per treatment combination after 4, 8 and 12 weeks of culture. Data was arc sine transformed for analysis. Shooting from explants was significantly higher (71.72%) on medium level of the BAP (0.5 mg/L) and IAA (0.2 mg/L) using in-vitro seedling stem as explant, however, it was 27.91% for stem bud as explant. Stem bud showed higher level of callusing (6.15%, p<0.001) in mandarin orange. However, flower bud didn't develop shoot in mandarin tissue culture. Increment of the in-vitro regeneration of the shooting and callusing was observed by the increment of the in-vitro incubation duration in mandarin orange tissue culture.

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Tissue culture parameters in sweet orange cultivars

Cited by 6 publications

References 10 publications

Growth regulators, culture media and antibiotics in the in vitro shoot regeneration from mature tissue of citrus cultivars

Growth regulators, culture media and antibiotics in the in vitro shoot regeneration from mature tissue of citrus cultivars

Role of auxins, polyamines and ethylene in root formation and growth in sweet orange

Hormonal Effect on Mandarin Orange (Citrus reticulata Blanco) Micro-propagation

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