Somatic embryogenesis and in vitro plant regeneration from pejibaye adult plant leaf primordia

Abstract

-The aim of this work was to evaluate a protocol for plant regeneration by means of somatic embryos obtained from isolated adult pejibaye leaf primordia, and to describe histological origin of embryos and morphogenetic response. Explants were cultivated in modified MS medium. Mesophyll parenchymatous cells originated meristemoids (preembryonic complex formation) induced with 7.1 µM BAP in the first two subculture periods. After polarized structures with 12.9 µM NAA and 3.55 µM BAP were formed in the third subculture, somatic embryos developed and regenerated normal plants. The mesophyll parenchymatous cells display high capacity of direct response to the auxin and cytokinin.Index terms: Bactris gasipaes, histology, micropropagation, peach palm. Embriogênese somática e regeneração de plantas in vitro a partir de primórdios foliares de pupunheiras adultasResumo -O objetivo deste trabalho foi avaliar um protocolo de regeneração de plantas por meio de embriões somáticos, obtidos a partir de primórdios foliares de pupunheiras adultas e identificar a origem histológica dos embriões e descrever as etapas morfogenéticas. Os explantes foram cultivados em meio MS modificado. Células parenquimáticas do mesofilo originaram meristemóides com BAP (7,1 µM) nos dois primeiros períodos de subcultura. A polarização das estruturas ocorreu com ANA (12,9 µM) e BAP (3,55 µM) no terceiro período de subcultura. Meristemóides se desenvolveram em embriões somáticos, regenerando plantas normais. As células parenquimáticas do mesofilo apresentam elevada capacidade de resposta direta à auxina e à citocinina.Termos para indexação: Bactris gasipaes, histologia, micropropagação.

TDZ pulsing evaluation on the in vitro morphogenesis of peach palm

“…Twenty seedlings were selected and cultivated in basal medium (MS -Murashige and Skoog 1962, modified by Almeida and Almeida 2006), followed by direct organogenesis induction on basal medium supplemented with α-naphthaleneacetic acid (NAA, 12.9 μM) and 6-benzylaminopurine (BAP, 3.55 μM). Subcultures were done twice, every 90 days, alternating with basal medium (Graner 2009).…”

“…Although palms are considered recalcitrant (Sarasan et al 2002;Steinmacher et al 2007), B. gasipaes in vitro regeneration through organogenesis and/or direct somatic embryogenesis has provided satisfactory results (Almeida and Kerbauy 1996;Almeida and Almeida 2006;Almeida et al 2012). However, further research is needed to optimize the propagation techniques.…”

Pre-procambial cells are niches for pluripotent and totipotent stem-like cells for organogenesis and somatic embryogenesis in the peach palm: a histological study

“…Histochemical monitoring was used to determine the essential factors involved in the acquisition of embryogenic competence in cells and/or tissues (Kouakou et al 2007;Pinto et al 2011;Rocha et al 2011) and the distinction of embryogenic competence from organogenetic events. The establishment of in vitro plant regeneration, through direct organogenesis or somatic embryogenesis in the peach palm, has been described (Almeida and Kerbauy 1996;Almeida and Almeida 2006;Graner 2009). Both morphogenic events can occur simultaneously in this species (Graner 2009); however, a better understanding of the morphogenic processes is needed to reveal the precise factors that trigger different scenarios during in vitro plant regeneration.…”

Anatomical analysis of peach palm (Bactris gasipaes) leaves cultivated in vitro, ex vitro and in vivo

“…Although a number of workers have investigated the in vitro multiplication of this species, including Almeida & Kerbauy (1996), Almeida et al (2005), Almeida & Almeida (2006), , micro-propagated seedlings are not easily encountered on a commercial scale due to high losses during their transfer to ex vitro conditions. Few workers have investigated acclimation of palm trees, and even fewer yet of peach palms.…”