Fatores de risco na transmissão do lentivírus caprino pelo sêmen

Andrioli, A.; Gouveia, Aurora Maria Guimarães; Martins, Almir de Sousa; Pinheiro, R. R.; Santos, D. O.

doi:10.1590/s0100-204x2006000800015

Cited by 31 publications

(28 citation statements)

References 20 publications

(12 reference statements)

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“…Thus, molecular techniques of viral detection have been used to provide an early diagnosis of the disease. One of these techniques is polymerase chain reaction (PCR), the aim of which is in vitro amplification of nucleic acids, thereby enabling the attainment of thousands of copies of a specific DNA sequence without the need for a bodily response such as the production of antibodies (ANDRIOLI et al, 2006).…”

Section: Resultsmentioning

confidence: 99%

“…This virus can lead to sub-clinical inflammation in one or more organs, mainly affecting the joints, nervous system, mammary glands, and respiratory system (BLACKLAWS et al, 2004). Caprine arthritis encephalitis (CAE) has been recorded worldwide (PETERHANS et al, 2004), is mainly found in dairy herds, and has been correlated with economic losses (ANDRIOLI et al, 2006). It is primarily transmitted to newborns via the colostrum; transmission can also occur via aerosol, animal-to-animal contact, and mating (PETERHANS et al, 2004;LE JAN et al, 2005).…”

Section: Introductionmentioning

confidence: 99%

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ANTI-Lentivirus, Brucella abortus AND B. ovis ANTIBODIES IN SMALL RUMINANTS RAISED IN PERNAMBUCO AND BAHIA

et al. 2016

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-Goat and sheep production in the semi-arid northeast of Brazil has shown great economic potential. However, health problems can compromise the productivity of these animals. Given the scarcity of studies about the occurrence of these diseases, the aim of the present study was to analyze the serological diagnosis of anti-Brucella and anti-lentivirus antibodies among small ruminants in municipalities located in the Brazilian states of Bahia and Pernambuco. The samples were collected from local slaughterhouses and dairy farms. In total, 997 serum samples from animals in slaughterhouses and dairy herds were collected. In order to diagnose the caprine arthritis encephalitis virus (CAEV), the samples underwent agarose gel immunodiffusion (AGID) testing. The buffered acidified antigen test (goats) and agarose gel immunodiffusion test (sheep) were used to detect anti-Brucella abortus and B. ovis antibodies following the methodology recommended by the Institute of Technology of Paraná (TECPAR). With anti-CAEV antibodies, seropositivity rates of 4.1% and 2.2% were recorded for animals from the slaughterhouses and dairy farms, respectively. None of the animals (goats or sheep) were positive for anti-B. abortus antibodies. With B. ovis, a seropositivity rate of 6.5% (n = 13) was recorded among the 199 sheep serum samples. Results of the present study confirmed the presence of the CAE virus in the meat and dairy herds studied, although the prevalence was low. Natural infection by B. abortus did not occur in the goat and sheep herds assessed. Seropositivity for B. ovis was confirmed, although prevalence was low. Direct tests are required to diagnose ovine brucellosis.Keywords: Brucellosis. CAEV. Goats. Sheep. Serology. ANTICORPOS ANTI-Lentivírus, Brucella abortus E B. ovis EM PEQUENOS RUMINANTES CRIADOS EM PERNAMBUCO E BAHIARESUMO -Alguns problemas de ordem sanitária ainda comprometem a produtividade de caprinos e ovinos. Sendo assim, foi realizado um estudo de diagnóstico sorológico da presença de anticorpos anti-lentivírus e antiBrucella em pequenos ruminantes localizados em municípios dos estados da Bahia e Pernambuco. As coletas foram realizadas em estabelecimentos de abate e em propriedades rurais com produção de leite, totalizando 997 amostras de soro. Para o diagnóstico do CAEV, as amostras foram submetidas ao teste de Imunodifusão em Gel de Agarose (IDGA). Para a pesquisa de anticorpos anti-Brucella abortus e Brucella ovis, foram utilizadas as técnicas do Antígeno Acidificado Tamponado (caprinos) e Imunodifusão em Gel de Agarose (ovinos), utilizando-se antígeno e metodologia recomendada pelo Instituto de Tecnologia do Paraná-TECPAR. Na pesquisa de anticorpos anti-CAEV, observou-se uma soropositividade de 4,1 e 2,2% para os animais oriundos de estabelecimentos de abate e de propriedades leiteiras, respectivamente. Quanto à pesquisa de anticorpos anti-B.abortus em caprinos e ovinos, não se observou nenhum animal positivo. Em relação a B. ovis, pesquisada em 199 amostras de soro ovino, obteve-se uma soroposivid...

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

ANTI-Lentivirus, Brucella abortus AND B. ovis ANTIBODIES IN SMALL RUMINANTS RAISED IN PERNAMBUCO AND BAHIA

et al. 2016

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“…A presença de patógenos no sêmen é um achado relevante, pois abre discussão sobre a possibilidade de transmissão venérea, implantando repercussão no manejo e nas biotécnicas reprodutivas atualmente aplicadas, principalmente quanto à inseminação artificial (IA) por ser cada vez mais utilizada por produtores de ovinos no Brasil (Hare 1985, Philpott 1994, Andrioli et al 2006.…”

Section: Discussionunclassified

“…No entanto, ainda pouco se sabe sobre o risco da transmissão de agentes patogênicos pelo sêmen (congelado ou diluído a fresco) com graves repercussões na produção e comercialização desses rebanhos (Andrioli et al 2006).…”

Section: Introductionunclassified

Detecção de Toxoplasma gondii no sêmen de ovinos naturalmente infectados

et al. 2010

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O objetivo deste trabalho foi estudar a eliminação de Toxoplasma gondii no sêmen de carneiros naturalmente infectados. Foram utilizados 65 reprodutores submetidos inicialmente à pesquisa de anticorpos anti-T. gondii por meio da técnica de imunofluorescência indireta (IFI). Os carneiros sorologicamente positivos foram submetidos à colheita de sêmen para detecção do DNA de T. gondii. Na sorologia observaram-se 6/65 (9,2%) carneiros positivos, enquanto no PCR nested de sêmen 4/6 (66,6%) carneiros foram positivos. Conclui-se que a detecção, por meio da técnica da PCR nested, da forma proliferativa de T. gondii no sêmen de carneiros naturalmente infectados, reforça a necessidade de se pesquisar sobre a possibilidade da transmissão horizontal do parasito via sêmen na espécie ovina.

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“…O CAEV também foi encontrado no sêmen e no órgão sexual de reprodutores (Andrioli et al, 2006;Peterson et al, 2008), o que sugere que a infecção venérea seja uma possibilidade. Souza et al (2013), visando testar esta hipótese, inseminaram 20 cabras com sêmen infectado artificialmente com o CAEV, e verificaram que todas soro converteram entre 30 e 60 dias após a inseminação.…”

Section: Introductionunclassified

Detecção do vírus da Artrite Encefalite Caprina por nested PCR e nested RT-PCR em ovócitos e fluido uterino

et al. 2013

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Caprine arthritis-encephalitis (CAE) is aninfectious disease caused by the caprine arthritis-encephalitis virus (CAEV), belonging to the lentivirus genus. The presence of the virus has been observed in the nervous system, respiratory tract and mammary gland, and also in the male and female genital tract. The objective of this study is to identify the virus in oocyte and uterine fluid of infected goats by molecular diagnostic techniques, in order to assess the possibility of CAEV transmission with reproduction. Thirteen infected goats were selected and submitted to euthanasia for the collection of the reproductive system, aspiration of the uterine fluid and dissection of ovaries for oocyte collection. In order to identify the CAEV in the collected material, in the protovirus and free forms, it was submitted to the nRT-PCR and nPCR techniques, respectively. As a result, it was observed that 53.8% of oocytes were positive to nRT-PCR, while only 9.1% were positive to nPCR. The nRT-PCR also identified the virus in the uterine fluid of 46.1% of the tested females. Even though the 13 goats had CAEV, 30.8% presented negative results in nPCR and nRT-PCR in all of the analyzed samples (oocyte and uterine fluid). This work concludes that nRT-PCR and nPCR can be used in the diagnosis of CAE for the analysis with oocytes and uterine fluid, and that the presence of CAEV in these materials points out to the risk of CAEV transmission through reproductive technologies used in females.

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Fatores de risco na transmissão do lentivírus caprino pelo sêmen

Cited by 31 publications

References 20 publications

ANTI-Lentivirus, Brucella abortus AND B. ovis ANTIBODIES IN SMALL RUMINANTS RAISED IN PERNAMBUCO AND BAHIA

ANTI-Lentivirus, Brucella abortus AND B. ovis ANTIBODIES IN SMALL RUMINANTS RAISED IN PERNAMBUCO AND BAHIA

Detecção de Toxoplasma gondii no sêmen de ovinos naturalmente infectados

Detecção do vírus da Artrite Encefalite Caprina por nested PCR e nested RT-PCR em ovócitos e fluido uterino

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