Influência da disponibilidade hídrica e da acidez do solo no teor de prolina livre de guandu

Marin, Adão; Santos, Durvalina Maria Mathias dos; Banzatto, David Ariovaldo; Codognotto, Lucas Mateus

doi:10.1590/s0100-204x2006000200023

Cited by 8 publications

(4 citation statements)

References 6 publications

(7 reference statements)

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“…It is known that a large number of citrus species accumulate both proline and proline betaine . Proline and proline betaine, which are synthesized by a two‐step methylation from proline, act as osmoprotectants and are responsible for protecting the cellular structure and for supporting physiological processes . The inverse correlation among sucrose and proline in T‐35S leaves suggests that the overproduction of proline results in an additional metabolic cost in the T‐35S plants and thereby reduces sucrose content.…”

Section: Resultsmentioning

confidence: 99%

HR‐MAS NMR metabolomics of ‘Swingle’ citrumelo rootstock genetically modified to overproduce proline

Oliveira

Carlos

Vieira

et al. 2014

Magnetic Reson in Chemistry

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The accumulation of proline is a typical physiological response to abiotic stresses in higher plants. 'Swingle' citrumelo, an important rootstock for citrus production, has been modified with a mutated Δ 1 -pyrroline-5-carboxylate synthetase gene (VaP5CSF129A) linked to the cauliflower mosaic virus 35S promoter to induce the overproduction of free proline. This paper presents a comparative metabolomic study of nontransgenic versus transgenic 'Swingle' citrumelo plants with high endogenous proline. 1 H high-resolution magic angle spinning nuclear magnetic resonance spectroscopy and multivariate analysis showed significant differences in some metabolites between the nontransgenic and transgenic leaves and roots. The overproduction of proline has reduced the sucrose content in transgenic leaves, revealing a metabolic cost for these plants. In roots, the high level of free proline acts for the adjustment of cation-anion balance, causing the reduction of acetic acid content. The same sucrose level in roots indicates that they can be considered as sucrose sink. Similar behavior may be waited for fruits produced on transgenic rootstock.

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Section: Resultsmentioning

confidence: 99%

HR‐MAS NMR metabolomics of ‘Swingle’ citrumelo rootstock genetically modified to overproduce proline

Oliveira

Carlos

Vieira

et al. 2014

Magnetic Reson in Chemistry

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“…One of the most studied plant responses to water deficit is the accumulation of free-proline, and many reports show a strong correlation between proline accumulation and drought tolerance increase, e.g. Nepomuceno et al, 2001 andMarin et al, 2006. Many other roles that indirectly help plants tolerate water shortage have been suggested for proline accumulation, like stabilizing sub-cellular structures, free-radical scavenging, energy storage and stress molecular signaling (Bartels & Sunkar, 2005).…”

Section: Resultsmentioning

confidence: 99%

Free proline accumulation in sugarcane under water restriction and spittlebug infestation

Guimarães

Mutton

et al. 2008

Sci. agric. (Piracicaba, Braz.)

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Mahanarva fimbriolata (Stål) (Hemiptera: Cercopidae) has become a key pest in the sugarcane (Saccharum officinarum) fields of center-south Brazil. Although some control technologies have shown to be efficient, the damage promoted by this spittlebug species and its interaction with sugarcane are poorly characterized. At high infestation levels the symptoms are similar to those of severe water restriction. This work was conducted to determine whether the stress promoted by spittlebug infestation can be measured in terms of free proline accumulation. The water restriction tolerance of two sugarcane genotypes was also compared. Two experiments were set up in a greenhouse and arranged in a completely randomized design in a 2 × 2 × 4 factorial, with two cultivars (SP80-1816 and RB72454), two stress levels (control and ten nymphs per plant or 50% water restriction), and four sampling dates. The water deficit caused by spittlebug nymphs sucking xylem sap does not result in proline accumulation, illustrating that there are different mechanisms to sense when the water deficit is caused by insect feeding or water potential variation in root cells. The cultivar RB72454 accumulates more free-proline, and the dry mass accumulation and stalk growth are less affected in this cultivar under water restriction. The levels of compatible solutes probably cannot be used to measure spittlebug infestation stress in sugarcane and RB72454 is more tolerant to water shortage than SP80-1816. Key words: Mahanarva fimbriolata, Saccharum spp., proline accumulation, water deficit ACÚMULO DE PROLINA EM CANA-DE-AÇÚCAR SOB ESTRESSE HÍDRICO OU SUBMETIDA AO ATAQUE DE CIGARRINHA-DAS-RAÍZESRESUMO: A cigarrinha-das-raízes Mahanarva fimbriolata (Stål) (Hemiptera: Cercopidae) tornou-se uma praga importante da cultura da cana-de-açúcar na região centro-sul do Brasil. Estratégias de controle têm sido desenvolvidas, mas os danos promovidos por essa praga e sua interação com a cana-de-açúcar ainda são pouco caracterizados. Em altos níveis de infestação da praga, os sintomas nas plantas são muito semelhantes à restrição hídrica severa. Este trabalho foi conduzido com o objetivo de determinar se o estresse promovido pela infestação de ninfas de cigarrinha-das-raízes resulta em acúmulo de prolina livre, além de comparar dois genótipos de cana-de-açúcar quanto à tolerância ao déficit hídrico. Dois experimentos foram conduzidos em delineamento inteiramente casualizado, em esquema fatorial 2 × 2 × 4 com duas cultivares (SP80-1816 e RB72454), dois níveis de estresse (controle e dez ninfas por planta ou 50% de restrição hídrica) e quatro épocas. O déficit hídrico promovido por M. fimbriolata não resultou em acúmulo de prolina, ilustrando a existência de mecanismos distintos para a percepção do estresse hídrico promovido pela praga e pela variação no potencial osmótico nas células das raízes. A cultivar RB72454 acumula maiores teores de prolina livre, e o acúmulo de massa seca e o crescimento dos colmos são menos afetados nessa cultivar sob restrição hídric...

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“…MDA was determined using the thiobarbituric acid assay method described by Health & Packer (1968) and modified by Erja et al (2001). The extraction and estimation of proline were done according to the methods of Bates et al (1973) and Marin et al (2006).…”

Section: Antioxidant Activitiesmentioning

confidence: 99%

Physiological and biochemical responses of selected cowpea (Vigna unguiculata (L.) Walp.) accessions to iron toxicity

Ifie

Ifie-Etumah

Ikhajiagbe

2020

AAS

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Physiological and biochemical responses of selected cowpea (Vigna unguiculata (L.) Walp.) accessions to iron toxicityAbstract: This study aimed to investigate the effect of iron toxicity in cowpea using physiological and biochemical responses of selected accessions. Fifteen accessions of cowpea were exposed to two treatments of iron using FeSO 4 solution (100 mg l −1 and 400 mg l −1 ) and distilled water at pH 6.2 as control. The results showed that there was a general reduction in germination morphology; germination percentage among the 400 mg l −1 Fe-treated accessions. Seed mortality rates were significantly higher among the 400 mg l −1 Fe-treated accessions (> 35 %). Water imbibition capacity and relative mass gained were higher for Fe-treated accessions. Furthermore, significant increase in the total sugar and percentage utilization of sugars was accompanied by an insignificant decrease in chlorophyll a, a significant decrease in chlorophyll b contents and the persistence of foliar chlorosis, among the 400 mg l −1 Fe-treated accessions. MDA levels were significantly increased while proline remained unchanged, mean SOD activity was insignificantly increased, whereas Cat decreased among the 400 mg l −1 Fetreated accessions. Documentation of these observable changes in physiological and biochemical parameters will be useful in understanding the impact of elevated iron concentrations on the cultivation of cowpea accessions in soils associated with ferruginous ultisols. Fiziološki in biokemični odziv akcesij kitajske vinje (Vigna unguiculata (L.) Walp.) na toksičnost železaIzvleček: Raziskava je bila izvedena z namenom preučevanja učinkov toksičnosti železa na kitajsko vinjo na osnovi fiziološkega in biokemičnega odziva izbranih akcesij. 15 akcesij kitajske vinje je bilo izpostavljeno dvema obravnavanjema z raztopino FeSO 4 (100 mg l −1 in 400 mg l −1 ) in destilirano vodo pri pH 6,2 kot kontrolo. Rezultati so pokazali, da je bilo splošno zmanjšanje v morfoloških parametrih kalitve in v odstotku kalitve pri akcesijah, ki so bile tretirane s 400 mg l −1 Fe. Tudi smrtnost semen je bila pri akcesijah, tretiranih s 400 mg l −1 Fe značilno večja (> 35 %). Sposobnost nabrekanja z vodo in relativno povečanje mase sta bila večja pri z železom tretiranih akcesijah. Nadalje je bilo opaženo pri s 400 mg l −1 Fe tretiranih akcesijah značilno povečanje vsebnosti celokupnih sladkorjev in njihove porabe, kar je bilo spremljano z neznačilnim upadom vsebnosti klorofila a, z značilnim upadom klorofila b in pojavom listnih kloroz. Pri obravnavanjih s 400 mg l −1 Fe se je vsebnost MDA značilno povečala, medtem, ko so vsebnosti prolina ostale nespremenjene, poprečna aktivnost SOD se je neznačilno povečala, aktivnost Cat pa zmanjšala. Dokumentiranje teh sprememb v fizioloških in biokemičnih parametrih bo koristno za razumevanje vpliva povečanih koncentracij železa pri gojenju akcesij kitajske vinje v tleh povezanih z železovimi ultisoli.Ključne besede: železov ultisol; Vigna unguiculata; akcesije kitajske vinje ; toksičnost železa; ra...

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Influência da disponibilidade hídrica e da acidez do solo no teor de prolina livre de guandu

Cited by 8 publications

References 6 publications

HR‐MAS NMR metabolomics of ‘Swingle’ citrumelo rootstock genetically modified to overproduce proline

HR‐MAS NMR metabolomics of ‘Swingle’ citrumelo rootstock genetically modified to overproduce proline

Free proline accumulation in sugarcane under water restriction and spittlebug infestation

Physiological and biochemical responses of selected cowpea (Vigna unguiculata (L.) Walp.) accessions to iron toxicity

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