HR‐MAS NMR metabolomics of ‘Swingle’ citrumelo rootstock genetically modified to overproduce proline

Oliveira, Caroline Silva de; Carlos, Eduardo Fermino; Vieira, Luiz Gonzaga Esteves; Lião, Luciano M.; Alcantara, Glaucia Braz

doi:10.1002/mrc.4082

Cited by 14 publications

(11 citation statements)

References 33 publications

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“…Mucci et al studied different tissues of lemons and citrons to understand the similarities and the differences between these two fruits [57]. In addition, it is possible to use metabolic profiling to characterise newly discovered plants [58,59] or mutants of plants [39,60,61]. The geographical origin of sweet peppers [62], garlic [63] and cocoa beans [64] has been investigated with HR-MAS NMR.…”

Section: Applications Of Hr-mas Nmr In Plant Metabolomicsmentioning

confidence: 99%

HR-MAS NMR Applications in Plant Metabolomics

2021

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Metabolomics is used to reduce the complexity of plants and to understand the underlying pathways of the plant phenotype. The metabolic profile of plants can be obtained by mass spectrometry or liquid-state NMR. The extraction of metabolites from the sample is necessary for both techniques to obtain the metabolic profile. This extraction step can be eliminated by making use of high-resolution magic angle spinning (HR-MAS) NMR. In this review, an HR-MAS NMR-based workflow is described in more detail, including used pulse sequences in metabolomics. The pre-processing steps of one-dimensional HR-MAS NMR spectra are presented, including spectral alignment, baseline correction, bucketing, normalisation and scaling procedures. We also highlight some of the models which can be used to perform multivariate analysis on the HR-MAS NMR spectra. Finally, applications of HR-MAS NMR in plant metabolomics are described and show that HR-MAS NMR is a powerful tool for plant metabolomics studies.

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Section: Applications Of Hr-mas Nmr In Plant Metabolomicsmentioning

confidence: 99%

HR-MAS NMR Applications in Plant Metabolomics

2021

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“…Moreover, the authors also reported an overexpression of quercetin and myricetin flavonoids in the transgenic beans. Roots and leaves of swingle citrumelo were investigated to prove the capability of a selected transgene to induce an overexpression of proline, an amino acid commonly associated with plant responses to certain environmental stress [50]. In particular, the authors used HRMAS to compare the metabolome of wild-type plants with that of transgenic T-35S plants carrying the VaP5CSF129A transgene encoding a mutated enzyme involved in proline biosynthesis.…”

Section: Plantsmentioning

confidence: 99%

“…In particular, the authors used HRMAS to compare the metabolome of wild-type plants with that of transgenic T-35S plants carrying the VaP5CSF129A transgene encoding a mutated enzyme involved in proline biosynthesis. Spectroscopic results showed that the transgenic plants exhibited a genotype-dependent response that entailed different levels of proline, proline betaine, sucrose, and acetic acid [50]. In another study, the composition of wild-type Arabidopsis thaliana was compared to that of cellulose-deficient mutant ectopic lignification1 through HRMAS-based experiments.…”

Section: Plantsmentioning

confidence: 99%

HRMAS NMR spectroscopy applications in agriculture

Mazzei¹,

Piccolo²

2017

Chem. Biol. Technol. Agric.

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The relatively recent and advanced high-resolution magic angle spinning (HRMAS) NMR technique enables the direct application of NMR spectroscopy to semi-solid and gel-like samples. It combines the advantages of both solid-and liquid-state NMR by allowing to concomitantly measure intact and non-manipulated samples. Based on both 1D and 2D homo-and heteronuclear NMR spectra, HRMAS evaluates the composition of fresh semi-solid samples with a similar resolution as that of classical liquid-state NMR techniques. The enhanced spectral quality still obtained for semi-solid samples is mainly due to the MAS system, whose rapid spinning and sample orientation minimize the anisotropic processes that prevent the acquisition of meaningful NMR spectra for non-liquid materials. Moreover, HRMAS allows us to use edited pulse sequences which, especially in the case of biological tissues or agrofood products, may provide a simultaneous information on polar and non-polar components without the need of preliminary sample extraction. Additionally, this technique may differentiate molecular species according to their degree of mobility in hydrated matrices. The evident versatile potential of the HRMAS NMR makes this technique particularly useful for life science molecular studies. Despite the focus of HRMAS has been greatly devoted on clinical biomedicine, materials chemistry, and metabolomics, there are already enough studies that show useful applications on agricultural issues. This report reviews the latest representative studies that employ HRMAS NMR on systems related to agricultural chemistry, requiring the characterization and dynamics of soil components, plant tissues, agrofood products, and in vivo organisms.

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“…Nuclear magnetic resonance (NMR) is a versatile technique in the study of small molecules and has been useful in several metabolomic studies . NMR combined with chemometrics has supported the investigation of biological systems under various conditions, in both animals and plants …”

Section: Introductionmentioning

confidence: 99%

NMR‐based approach reveals seasonal metabolic changes in mate (Ilex paraguariensis A. St.‐Hil.)

Freitas

Nunes

Apparecido

et al. 2018

Magnetic Reson in Chemistry

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Ilex paraguariensis (mate) is a species native to South America and is widely consumed in countries such Argentina, Uruguay, Paraguay, and Brazil. Mate consumption is associated with several phytotherapeutic functions, in addition to its cultural and regional importance. However, the harvest period can affect the properties of the mate, due to variations in the constituent proportions, as a consequence of seasonal changes. In this work, we employed nuclear magnetic resonance and chemometrics to evaluate the chemical variations in leaf extracts of I. paraguariensis over the four seasons of the year. We found significant changes in the levels of glucose, myo-inositol, caffeine, theobromine, and fatty acids. These changes can be related to resource allocation for the flowering period, or to responses to environmental stresses, such as temperature.

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HR‐MAS NMR metabolomics of ‘Swingle’ citrumelo rootstock genetically modified to overproduce proline

Cited by 14 publications

References 33 publications

HR-MAS NMR Applications in Plant Metabolomics

HR-MAS NMR Applications in Plant Metabolomics

HRMAS NMR spectroscopy applications in agriculture

NMR‐based approach reveals seasonal metabolic changes in mate (Ilex paraguariensis A. St.‐Hil.)

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