Controle de nematóides parasitos gastrintestinais por Arthrobotrys musiformis em ovinos naturalmente infestados mantidos em pastagens

Graminha, Érika Barbosa Neves; Monteiro, Ana Ruth Macêdo; Silva, Heloísa Cristina da; Oliveira, Gilson Pereira de; Costa, Alvimar José da

doi:10.1590/s0100-204x2005000900013

Cited by 14 publications

(8 citation statements)

References 9 publications

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“…Animals then consume the spores along with the grass, as a result, these strains could even adapt to adverse conditions of the gastro-intestinal tract of animals, which can be interpreted as the positive result in the assay (Orozco 2005). A. conoides did not survive the digestibility assay; this coincides with Graminha et al (2005b) who obtained the same results in his investigation of A. conoides and C. musiformis.…”

Section: Predation Assaysupporting

confidence: 88%

In-vitro predatory activity of nematophagous fungi from Costa Rica with potential use for controlling sheep and goat parasitic nematodes

Soto-Barrientos¹,

Oliveira²,

Vega-Obando³

et al. 2010

RBT

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In tropical and subtropical regions of the world, parasitic diseases are a main cause of losses in livestock productivity. The increased acquired resistence to anthelmintics by gastrointestinal nematodes, requires biological control be considered as a potential feasible and effective alternative. The most effective natural soil enemies of nematodes are nematophagous fungi. In order to collect and identify predator nematophagous fungi (PNF), samples were obtained from 51 farms distributed throughout the seven provinces of Costa Rica. The origin samples included: soil from different crops (potatoes, tomatoes, bananas, ornamental plants, squash and coffee); animal feces (cattle, sheep, goat and horse); soil and fallen leaves from forest; and plants with signs of nematode infection. Each sample was processed using three techniques for the extraction of fungi from soil: sprinkling technique, soil dilution and humidity chamber. Twenty four strains of nematophagous fungi were found in 19 farms; 83.3% of the fungi were isolated by sprinkling technique. The following fungi were idenified: Arthrobotrys oligospora (n=13); Candelabrella musiformis (n=9); and for the first time there was isolation of A. conoides (n=1) and A. dactyloides (n=1) in the country. Moreover, 16 strains from Trichoderma (n=13), Beauveria (n=1), Clonostachys (n=1) and Lecanicillium (n=1) were obtained. In addition, pH of each possible fungal isolation source was measured, and it varied from 5.2 to 9.9, however PNF isolates fell within the range of 5.6 to 7.5. The PNF strains were cultivated in four different media for the production of chhlamydospores: potato dextrose agar (PDA); corn meal agar (CMA); malt extract agar (MEA) and potato carrot agar (PCA). Out of these cultures, 95.8% of the strains formed chlamydospores primarily in the PCA. Of these strains, the profilic spore producers were subjected to ruminant artificial gastrointestinal conditions. A total of 14 fungi were tested, out of which 42.9% survived the digestive analysis. Neither A. conoides nor A. dactyloides were viable following the in vitro gastrointestinal test. The PNF isolated in this study demostrated an action against ovine and caprine gastrointestinal nematodes and are candidates for use in biological control of these organisms. Among these microorganisms, Candelabrella musiformis appears to be the most promising fungi for use as a biological control agent in

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Section: Predation Assaysupporting

confidence: 88%

In-vitro predatory activity of nematophagous fungi from Costa Rica with potential use for controlling sheep and goat parasitic nematodes

Soto-Barrientos¹,

Oliveira²,

Vega-Obando³

et al. 2010

RBT

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“…Alves et al (2003) and Araújo et al (2004b) observed the efficacy of a fungal isolate of M. thaumasium, with a reduction in the number of eggs per gram of feces (EPG) in treated animals ranging from 88% to 100%. Graminha et al (2005) evaluated the oral administration of the fungus Arthrobotrys musiformis (conidia microencapsulated in alginate or in natural liquid medium) for sheep. The results demonstrated that the fungus was effective even after passage through the gastrointestinal tract of sheep, and showed no change in its predatory capacity for larvae of nematode parasites.…”

Section: Resultsmentioning

confidence: 99%

In vitro nematophagous activity of predatory fungi on infective larvae of Strongyloides papillosus

Campos

Valadão

Carvalho

et al. 2017

AVB

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An in vitro experiment was carried out to assess the effect of fungal isolates of the genus Arthrobotrys, Duddingtonia, Monacrosporium and Nematoctonus on infective Strongyloides papillosus larvae. A suspension of 1,000 infective S. papillosus larvae was dripped on to Petri dishes containing fungi grown in agar-water. There was a statistically significant difference (p<0.05) among all the isolates and the fungus-free control. Comparing the results between isolates, difference was observed in some isolates of the genus Arthrobotrys (CG 719, I40, A1, A2, C1, B1), D. flagrans (CG722), Monacrosporium (CGI, SF53, NF34A) and N. robustus (D1) that showed a better capture and destruction results and consequently a greater decrease in the average of the uncaptured larvae recovered. The results showed that there may be variations in the predatory capacity of different species and among fungal isolates of the same species on Strongyloides papillosus and indicated that nematophagous fungi may be used for the biological control of this helminth. WALLER, 1998;JACOBS et al., 2016;TAYLOR et al., 2016). Infections by this parasite can cause gastrointestinal disturbances (enteritis, diarrhea and loss of appetite), affect body weight and delay growth and sudden death syndrome can occur (MEHLHORN, 2016;PIENNAR et al., 1999;TAIRA et al., 1992).In Brazil, benzimidazole and macrocyclic lactone anthelmintics are the principal forms of control used against gastrointestinal nematode parasites of ruminants (BOWMAN, 2010;CHARLES;FURLONG, 1996). However, the public health significance of anthelmintic drug residues in products of animal origin, the presence of these drugs in animal feces, their negative influence on biodiversity and agricultural ecosystem sustainability (due to the heavy reliance on systematic and sometimes abusive therapeutic use) and reports of helminth strains tolerant or resistant to different anthelmintic groups have triggered investigation into alternative methods for the sustainable control of gastrointestinal nematodes of ruminants (CAI et al., 2016;GRONVOLD et al., 1996;SUAREZ, 2002). The non-chemotherapeutic approach to the control of nematode gastrointestinal parasites of ruminants using nematophagous fungi as biological Campos et al. Acta Veterinaria Brasilica March 11 (2017) [213][214][215][216][217][218] 214 control agents showed consistent results (LARSEN, 2000;PAZ-SILVA et al., 2011). Because of differences in the nematophagous capacities of these fungi, studies to distinguish the species and the variations among them are required (ARAÚJO et al., 2004a;CARVALHO;BERNARDO;.The purpose of this study was to evaluate the activity of Brazilian isolates of nematophagous fungi of the Arthrobotrys, Duddingtonia, Monacrosporium and Nematoctonus genera against S. papillosus larvae in laboratory conditions. MATERIALS AND METHODSCultures of 17 fungal isolates of the Arthrobotrys, Duddingtonia, Monacrosporium and Nematoctonus genera (Table 1), grown on 1.7% corn meal agar culture media (CMA, DIFCO ® ) in test tubes, we...

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“…are effective in reducing infective larvae of cyathostomin (Castro et al 2003), H. contortus (Eslami et al 2005), and Cooperia punctata (Araújo and Guimarães 2002) and should be considered as a biological control agent for integrated nematode control programs. In Brazil, Graminha et al (2005) studied the efficacy of Arthrobotrys musiformis fungus in naturally infected sheep and did not detect differences between the FEC of the control group and that of the treated group; however, they observed that A. musiformis reduced the number of T. colubriformis by more than 50% in tracer lambs. In the present study, the group that received A. robusta fungus also received less anthelmintic treatments and, therefore, the A. robusta fungus should be further investigated to evaluate its potential in biological control.…”

Section: Discussionmentioning

confidence: 97%

Efficacy of Duddingtonia flagrans and Arthrobotrys robusta in controlling sheep parasitic gastroenteritis

et al. 2010

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The aim of this study was to evaluate the efficacy of formulations of sodium alginate matrix (pellets) of the nematode predatory fungi, Duddingtonia flagrans (AC001 isolate) and Arthrobotrys robusta (I-31 isolate), in the biological control of sheep gastrointestinal nematode infections. Thirty young Bergamacia ewes were allocated into three groups: In group 1 (control), the animals received 2 g/10 kg of live weight (l.w.) of pellets without fungus; in group 2, each animal received 2 g/10 kg of l.w. of pellets of D. flagrans (0.2 g of fungus/10 kg l.w.); and in group 3, each animal received 2 g/10 kg of l.w. of pellets of A. robusta (0.2 g of fungus/10 kg l.w.). The animals of each group were kept separately under rotational grazing. Pellets, with or without fungi, were mixed with 1 kg animal food and administered twice a week for 6 months. There was no significant difference in mean live weight and packed cell volume among groups (P > 0.05). Mean nematode fecal egg counts (FEC) did not significantly differ between the control and the remaining groups, except in one or two collections, when FEC was higher in the control group than in group 2 and group 3, respectively. The group that received A. robusta pellets needed less salvage anthelmintic treatments. Haemonchus contortus was the predominant species recovered from tracer lambs. The nematophagous fungi, D. flagrans and A. robusta, did not provide satisfactory results in the prophylaxis of parasitic gastroenteritis in sheep, under the conditions of the present study.

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Controle de nematóides parasitos gastrintestinais por Arthrobotrys musiformis em ovinos naturalmente infestados mantidos em pastagens

Cited by 14 publications

References 9 publications

In-vitro predatory activity of nematophagous fungi from Costa Rica with potential use for controlling sheep and goat parasitic nematodes

In-vitro predatory activity of nematophagous fungi from Costa Rica with potential use for controlling sheep and goat parasitic nematodes

In vitro nematophagous activity of predatory fungi on infective larvae of Strongyloides papillosus

Efficacy of Duddingtonia flagrans and Arthrobotrys robusta in controlling sheep parasitic gastroenteritis

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