Caracterização genética de rizóbios nativos dos tabuleiros costeiros eficientes em culturas do guandu e caupi

Fernandes, Marcelo Ferreira; Fernandes, Roberta Pereira Miranda; Hungría, Mariangela

doi:10.1590/s0100-204x2003000800003

Cited by 13 publications

(6 citation statements)

References 27 publications

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“…The restriction profiles analysis obtained for all the Amazon isolates with the enzymes Msp I and Dde I were the same but Hinf I detected differences that separated them into three subgroups. Isolates that had identical rDNA genotypes did not display similar phenotypic characteristics (3) and it was corroborated by our results. In the dendrogram (Figure 1), the first subgroup of Amazon isolates assembled 16 isolates from 10 cultural groups with 100% of similarity; the second grouped 4 isolates from 4 cultural groups, also with 100% of similarity and the third was composed by only one isolate.…”

Section: Resultssupporting

confidence: 87%

“…The ARDRA method has efficiency in genetic characterization and diversity study of rhizobia (30, 2) and it was corroborated by ours results since the isolates from the same cultural group, for example, (BR3306 and BR3309), (BR3307 and BR3310) and (BR3353, BR3316 and BR3336) were separated into first and second subgroups (Figure 1). It means that genetic diversity was detected by ARDRA technique, although it is not always able to separate species that are closely related (3). The restriction profiles obtained with the three enzymes Hinf I, Msp I and Dde I pointed differences among the reference isolates which separated the Amazon isolates from the Bradyrhizobium japonicum (BR33) with 70% of similarity and from the Bradyrhizobium elkanii (BR29, BR96, BR2001) strain with 50% of similarity (Figure 1).…”

Section: Resultsmentioning

confidence: 99%

“…In the literature, the Bradyrhizobium isolates have shown slow-growing or fast-growing characteristics (22, 3). …”

Section: Resultsmentioning

confidence: 99%

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Genetic diversity of Rhizobia isolates from Amazon soils using cowpea (Vigna unguiculata) as trap plant

Silva¹,

Simões‐Araújo²,

Júnior³

et al. 2012

Braz. J. Microbiol.

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The aim of this work was to characterize rhizobia isolated from the root nodules of cowpea (Vigna unguiculata) plants cultivated in Amazon soils samples by means of ARDRA (Amplified rDNA Restriction Analysis) and sequencing analysis, to know their phylogenetic relationships. The 16S rRNA gene of rhizobia was amplified by PCR (polymerase chain reaction) using universal primers Y1 and Y3. The amplification products were analyzed by the restriction enzymes HinfI, MspI and DdeI and also sequenced with Y1, Y3 and six intermediate primers. The clustering analysis based on ARDRA profiles separated the Amazon isolates in three subgroups, which formed a group apart from the reference isolates of Bradyrhizobium japonicum and Bradyrhizobium elkanii. The clustering analysis of 16S rRNA gene sequences showed that the fast-growing isolates had similarity with Enterobacter, Rhizobium, Klebsiella and Bradyrhizobium and all the slow-growing clustered close to Bradyrhizobium.

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Section: Resultssupporting

confidence: 87%

Section: Resultsmentioning

confidence: 99%

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Genetic diversity of Rhizobia isolates from Amazon soils using cowpea (Vigna unguiculata) as trap plant

Silva¹,

Simões‐Araújo²,

Júnior³

et al. 2012

Braz. J. Microbiol.

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“…Embora tenham sido observados alguns agrupamentos entre bactérias provenientes de tratamentos sob o mesmo manejo de solo ou de culturas, de modo geral, não houve agrupamentos relacionados aos tratamentos (Figura 2). Laguerre et al (1997) salientaram que a análise com "primers" de regiões conservadas e repetidas permite a identificação de estirpes, podendo não haver agrupamento de espécies, o que também foi confirmado em outros estudos com rizóbios (Fernandes et al, 2003;Kaschuk et al, 2006a,b). Desse modo, neste estudo, considerando o nível de similaridade de 70%, foram obtidos 42 perfis de BOX-PCR, que corresponderiam a diferentes estirpes (Figura 2).…”

Section: Diversidade Genética De Rizóbiosunclassified

Variações qualitativas e quantitativas na microbiota do solo e na fixação biológica do nitrogênio sob diferentes manejos com soja

Pereira

Hungría

Franchini

et al. 2007

Rev. Bras. Ciênc. Solo

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RESUMONeste estudo foram avaliados atributos qualitativos e quantitativos da microbiota do solo, visando monitorar alterações por diferentes manejos do solo e das culturas. As avaliações foram feitas em um ensaio a campo, conduzido há 14 anos em Londrina, PR, sob plantio convencional (PC) ou plantio direto (PD) e com sucessão (S) (soja/trigo) ou rotação (R) (tremoço/milho/aveia-preta/soja/trigo/soja/ trigo/soja) de culturas, quando todos os sistemas estavam com soja no estádio de florescimento pleno. Os incrementos no C e N da biomassa microbiana (CBM e NBM) no PD foram de 114 e 157 %, respectivamente, em comparação ao PC; além disso, o quociente metabólico (qCO 2 ) foi inferior em 37 % no PD, indicando maior eficiência metabólica da microbiota do solo. Não foram detectadas diferenças nesses atributos em função dos sistemas de rotação e sucessão de culturas. A diversidade genética da comunidade bacteriana total do solo foi superior no PD e inferior no PC com sucessão de culturas. Em relação à fixação biológica do N 2 , a massa, o N total e a fração de N-ureídos acumulados na parte aérea e a eficiência dos nódulos em fixar N 2 foram superiores no PD. A diversidade genética dos rizóbios foi afetada, principalmente, pelo manejo das culturas, sendo superior com a rotação, provavelmente pelo maior número de espécies de plantas. Contudo, com a rotação ocorreu decréscimo na eficiência do processo de fixação biológica do N 2 , o que pode estar relacionado com os teores mais elevados de N no solo, ou com a menor

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“…Genetic diversity is evaluated by several other advance molecular methods e.g., ADRDA, ERIC-PCR which enables the differentiation between species and strain of the same species (Pinto et al 2007;Wang et al 2009). RFLP-PCR associated with ribosomal genes is another useful technique that has been used for phylogenetic studies, as it can discriminate rhizobial species and generally exhibits good agreement with partial or complete gene sequencing data (Laguerre et al 1996;Vinuesa et al 1998;Diouf et al 2000;Andrade et al 2002;Fernandes et al 2003;Pandey et al 2004;Moschetti et al 2005;Kumar et al 2006). Pisa et al (2011) explored diversity of 16S rRNA gene from bacteria of sugarcane rhizosphere.…”

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confidence: 99%

Trends and Prospects of Microbial Diversity in Rhizosphere

Maheshwari

Agarwal

Dheeman

2014

Sustainable Development and Biodiversity

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Biodiversity is an appealing aspect including microorganisms encompasses diverse assemblage of organisms exhibiting with morphological, physiological, biochemical, metabolic and ecological diversity. At the level of taxonomic diversity microorganism signify phenotypic and genotypic diversity. Whereas, phenotypic diversity physiologically diversify the population of bacteria on the other hand, genotypic diversity explore the genetic divergence within the bacterial population. Functional diversity in the rhizosphere in particular is also considered as functional behavior of microbial community in their ecological niche or ecosystem. Functional diversity is the measures of distribution and functional niche of organisms where they remain functional in communities and multi-dimensional cloud of species trait and each trait representing an individual or a species. Most often define biodiversity as the "totality of genes, species, and ecosystems of a region studied under term species diversity, genetic diversity and ecological diversity." Microbial diversity in rhizosphere bears several interactions and controls the functional roles to permit parse and plant survival. Measurement of biodiversity on species richness and evenness/dominance indices, species abundance models is recent tools to assess microbial diversity. Rhizosphere implicit microbial population aids benefits for plants in term of growth promotion and proved itself as important and putative communities for increased crop productivity. IntroductionThe term biodiversity was used first by Wilson (1988). Since then the concept of biodiversity continuous spread to arose as developing and emerging field. Biodiversity is defined as the variety of living organisms at species, inter-species and intraspecies level in ecology which is much considered in terms of plants and animals; yet, the assortment of microbial life forms a huge and largely uncharted account. Microorganisms are quite beneficial and exploited in gain of quite lot revenue for D. K. Maheshwari et al. 2 humankind and earth's ecology. Similarly, as the plants and animals are conserved to counter the alarm of extinction of their species, the great reservoir of rhizospheric microbial diversity, employed for ecological and humankind services should be conserved in the means of culture collection and genomic library construction and utilization in agriculture. Such appealing organisms having distributional merit in all three dimensions of nature and ecology viz., soil, water and air, where microbial diversity signify at distinct ecological levels. The soil is a reservoir and prime nutritional habitat of severe community of phenotypic and genotypic diverse microorganisms. It is daunting task to grasp considerate of microbial diversity so, under this quest, it is necessary to group such organisms together so as to organize them in non-overlapping hierarchy. On account of classification system using polyphasic taxonomy i.e., phenotypic, genotypic and phylogenetic classification results diversity a...

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Caracterização genética de rizóbios nativos dos tabuleiros costeiros eficientes em culturas do guandu e caupi

Cited by 13 publications

References 27 publications

Genetic diversity of Rhizobia isolates from Amazon soils using cowpea (Vigna unguiculata) as trap plant

Genetic diversity of Rhizobia isolates from Amazon soils using cowpea (Vigna unguiculata) as trap plant

Variações qualitativas e quantitativas na microbiota do solo e na fixação biológica do nitrogênio sob diferentes manejos com soja

Trends and Prospects of Microbial Diversity in Rhizosphere

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