2012
DOI: 10.1590/s1517-83822012000200033
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Abstract: The aim of this work was to characterize rhizobia isolated from the root nodules of cowpea (Vigna unguiculata) plants cultivated in Amazon soils samples by means of ARDRA (Amplified rDNA Restriction Analysis) and sequencing analysis, to know their phylogenetic relationships. The 16S rRNA gene of rhizobia was amplified by PCR (polymerase chain reaction) using universal primers Y1 and Y3. The amplification products were analyzed by the restriction enzymes HinfI, MspI and DdeI and also sequenced with Y1, Y3 and s… Show more

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Cited by 28 publications
(37 citation statements)
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“…However, it is currently classified into genus Bradyrhizobium [8,19]. Phenotypic and genetic studies of cowpeas rhizobia were aim of researchers in some parts of Africa [20][21][22][23][24][25][26]. Contradictory reports about the microsymbionts of these plants were reported.…”
Section: Introductionmentioning
confidence: 99%
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“…However, it is currently classified into genus Bradyrhizobium [8,19]. Phenotypic and genetic studies of cowpeas rhizobia were aim of researchers in some parts of Africa [20][21][22][23][24][25][26]. Contradictory reports about the microsymbionts of these plants were reported.…”
Section: Introductionmentioning
confidence: 99%
“…Contradictory reports about the microsymbionts of these plants were reported. Some investigators indicates that cowpea nodulated by both fast and slow growing rhizobia [20][21][22][23][24]. Analysis of 16S rRNA gene sequences of fast-growing nodule occupancy showed that they are similar to coliform bacteria (Enterobacter, Klebsiella) and Rhizobium, while, the slow-growing one is close to Bradyrhizobium genus [24].…”
Section: Introductionmentioning
confidence: 99%
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“…Although there was a prevalence of species of the genus Bradyrhizobium, bacteria from the genera Rhizobium and Burkholderia have been also found. Silva et al (2012) investigated soils collected from six AR areas (2 u 529 52.750 N 59 u 599 40.110 W) with different cultivation histories, including farm land and native noncropped areas using Vigna unguiculata as the trap plant. They isolated more than 100 strains, which were slowgrowing on YMA medium (Fred & Waksman, 1928) and 16S rRNA sequence analysis confirmed that 46 of them belonged to the genus Bradyrhizobium .…”
mentioning
confidence: 99%
“…Bradyrhizobium species are Gram-negative bacilli (rod shaped) with a single subpolar or polar flagellum. Many changes in rhizobia taxonomy have occurred during the last decade due to an increase of available phenetic and genetic information about this group of bacteria (Silva et al, 2012). Knowledge of the diversity of rhizobia is of paramount importance as it is a source of genetic resources for selection of strains adapted to different conditions.…”
Section: Introductionmentioning
confidence: 99%