Embriogênese somática in vitro em couve-comum

Abstract

RESUMO -O objetivo deste estudo foi obter calos e embriões somáticos de couve-comum (Brassica oleracea L., var. leucocephala) e caracterizá-los por meio de observações histológicas. Foram cultivadas, in vitro, explantes foliares, sob condições assépticas, em meio nutritivo MS, suplementado com várias combinações entre 2,4-D/BAP e 2,4-D/KIN. Na fase Termos para indexação: cultura de tecidos, calos, embriões somáticos. IN VITRO SOMATIC EMBRYOGENESIS OF COMMON CABBAGEABSTRACT -The aim of this study was to obtain calluses and somatic embryos of common cabbage (Brassica oleracea L., var. leucocephala) and to characterize them by means of histological observations in vitro; foliar explants were cultivated under asseptic conditions in a nutritive MS medium , that were chosen for subcultivation in regeneration media (phase II). In this phase, culture media were supplemented with reduced concentrations of 2,4-D, BAP and GA 3 , or they were totally deprived of these supplements. In this case, the calluses remained in cultivation for other 30 days. The calluses that were subcultivated in the regeneration media R3 (0.1 mg/L of BAP) and R8 (0.001 mg/L of 2,4-D plus 0.01 mg/L of BAP), derived from the induction media M16 (5.0 mg/L of 2,4-D) and M30 (1.0 and 1.0 mg/L of 2,4-D and BAP), respectively, developed structures similar to embryos. However, this histological characterization revealed the superiority of the interaction between the M30 and R8 media for regeneration of the calluses and somatic embryos.

Effects of somatic embryogenesis on gene expression of cloned coffee heterozygous hybrids

“…and indirect, where somatic embryos originated from callus, a mass of cells with disorganized growth. The methodology of indirect somatic embryogenesis is the most promising in coffee, but still requires studies for adaptation to the reality of large-scale production (Donato et al 2000;Menéndez-Yuffá et al 2010).…”

In vitro callus induction and development of Vernonia condensata Baker with embryogenic potential

“…The medium supplemented with 2.22 μM BAP favored the development of more calli with score 3, classified as medium (0.8 to 1.0 cm) from internodal segments. Donato et al (2000), evaluating somatic embryogenesis in vitro in common cabbage (Brassica oleracea L., var. leucocephala) did not observe a significant difference regarding the intensity of callus formation, both in the treatments that combined 2,4-D and KIN and in the plants treated with the association of 2,4-D and BAP.…”