Anatomical Studies of in Vitro Organogenesis Induced in Leaf-Derived Explants of Passionfruit

Glória, Beatriz Appezzato da; Vieira, Maria Lúcia Carneiro; Dornelas, Marcelo Carnier

doi:10.1590/s0100-204x1999001100005

Cited by 27 publications

(9 citation statements)

References 18 publications

(26 reference statements)

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“…Studies involving highly comprehensive and descriptive anatomical analyses of in vitro organogenesis in Passiflora species have been published (Appezzato-da-Glória et al 1999, 2005Fernando et al 2007;Lombardi et al 2007). However, despite the importance of these studies for the characterization of somatic embryogenesis in the genus, little has been reported.…”

Section: Introductionmentioning

confidence: 99%

Somatic embryogenesis of a wild passion fruit species Passiflora cincinnata Masters: histocytological and histochemical evidences

et al. 2011

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The characterization of cellular changes that occur during somatic embryogenesis is essential for understanding the factors involved in the transition of somatic cells into embryogenically competent cells and determination of cells and/or tissues involved. The present study describes the anatomical and ultrastructural events that lead to the formation of somatic embryos in the model system of the wild passion fruit (Passiflora cincinnata). Mature zygotic embryos were inoculated in Murashige and Skoog induction media supplemented with 2,4-dichlorophenoxyacetic acid and 6-benzyladenine. Zygotic embryo explants at different development stages were collected and processed by conventional methods for studies using light, scanning, and transmission electron microscopy (TEM). Histochemical tests were used to examine the mobilization of reserves. The differentiation of the somatic embryos began in the abaxial side of the cotyledon region. Protuberances were formed from the meristematic proliferation of the epidermal and mesophyll cells. These cells had large nuclei, dense cytoplasm with a predominance of mitochondria, and a few reserve compounds. The protuberances extended throughout the abaxial surface of the cotyledons. The ongoing differentiation of peripheral cells of these structures led to the formation of proembryogenic zones, which, in turn, dedifferentiated into somatic embryos of multicellular origin. In the initial stages of embryogenesis, the epidermal and mesophyll cells showed starch grains and less lipids and protein reserves than the starting explant. These results provide detailed information on anatomical and ultrastructural changes involved in the acquisition of embryogenic competence and embryo differentiation that has been lacking so far in Passiflora.

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Section: Introductionmentioning

confidence: 99%

Somatic embryogenesis of a wild passion fruit species Passiflora cincinnata Masters: histocytological and histochemical evidences

et al. 2011

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“…Existem vários trabalhos descrevendo a anatomia da folha para representantes do subgênero Passiflora, como em P. suberosa (Jauregui et al 2002;Barp et al 2006;Giani 2009), P. edulis (Gloria et al 1999;Beraldo & Kato 2008;Cop et al 2014), P. foetida (Jauregui et al 2002;Machado et al 2008;Costa 2013), P. misera (Giani 2009;Cop et al 2014), P. campanulata (Meruvia et al 1993a), P. actinia (Kurtz et al 2003), P. lobata (Cardoso 2008), P. quadrangularis (Freitas 1985), P. incarnata (Freitas 1985), P. amenthystina (Rocha et al 2009) e P. adenopoda (Gilbert 1971).…”

Section: Discussionunclassified

“…Todas as espécies analisadas apresentaram epiderme uniestratificada, o que corrobora as descrições já realizadas para o grupo (Gloria et al 1999). Além disso, algumas espécies apresentam células papilosas na face abaxial da epiderme, característica esta que foi citada por Metcalfe & Chalk (1950) para Passiflora e Adenia (Passifloraceae).…”

Section: Discussionunclassified

“…Metcalfe & Chalk (1950) mencionaram que pode ocorrer, também, um mesofilo isobilateral em Passiflora ou em outros gêneros da família como em Adenia, Paropsia e Tryphostemma. Assim como foi observado no presente estudo, o mesofilo é formado, geralmente, por uma camada de células paliçádicas e por cerca de seis a sete estratos compondo o parênquima lacunoso, o que corrobora o estudo realizado em outras espécies de Passiflora (Meruvia et al 1993a;Gloria et al 1999;Barp et al 2006;Beraldo & Kato 2008;Leite et al 2013). Embora os autores mencionados acima tenham observado variações na estrutura do mesofilo, no presente estudo as espécies mostraram-se homogêneas quanto a esta característica, o que pode indicar um valor taxonômico para essa característica, para o subgênero.…”

Section: Discussionunclassified

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Anatomia foliar de Passiflora subgênero Decaloba (Passifloraceae): implicações taxonômicas

et al. 2016

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ResumoPassifloraceae possui 17 gêneros e cerca de 750 espécies. O gênero Passiflora, possui aproximadamente 525 espécies, distribuídas nas regiões tropicais do Novo Mundo, Ásia e Austrália, e está subdividido em quatro subgêneros: Astrophea, Decaloba, Passiflora e Deidamioides. Este trabalho teve por objetivo verificar a ocorrência de caracteres diagnósticos e fornecer subsídios para a taxonomia do grupo. Para tanto, foram analisadas oito espécies: Passiflora capsularis, Passiflora misera, Passiflora morifolia, Passiflora organensis, Passiflora pohlii, Passiflora suberosa, Passiflora tricuspis e Passiflora vespertilio. Foram estudados de três a seis indivíduos de cada espécie, obtidos a partir das coleções herborizadas dos herbários do Departamento de Botânica da UFPR (UPCB) e do Museu Botânico Municipal (MBM), ambos em Curitiba-PR. Para o estudo anatômico, as amostras foliares foram submetidas ao processo de reidratação e, posteriormente, incluidas em historesina seguindo técnicas usuais utilizadas em anatomia vegetal. Alguns caracteres se destacaram com valor taxonômico e com potencial uso em estudos filogenéticos, como a presença de papilas epidérmicas, cutícula ornamentada e sistema vascular em arco. Os resultados indicam que estudos mais detalhados sobre a estrutura das papilas epidérmicas e dos tipos de tricomas tectores trarão mais informações para o entendimento das relações em Decaloba e os outros subgenêros. Palavras-chave: estruturas secretoras, folha, papilas, taxonomia. AbstractPassifloraceae comprises 17 genera and approximately 750 species. The genus Passiflora has about 525 species distributed in tropical regions of the New World, Asia and Australia. Passiflora is subdivided in four subgenera: Astrophea, Decaloba, Passiflora, Deidamioides. The goal of this work is to verify the utility of selected anatomical features as diagnose or taxonomical characters. Three to six individuals of eight species of Passiflora, subgenus Decaloba were used: Passiflora capsularis, Passiflora misera, Passiflora morifolia, Passiflora organensis, Passiflora pohlii, Passiflora suberosa, Passiflora tricuspis and Passiflora vespertilio. Samples were obtained from Herbarium collections of the Herbarium of the Departamento de Botânica da Universidade Federal do Paraná (UPCB) and Museu Botânico Municipal (MBM), Curitiba-PR. For the anatomical study, leaf samples were embedded in Historesin following usual anatomical procedures. Some features can be highlighted as taxonomic useful, and of potential use in phylogenetic studies, such as ornamented cuticle, presence of epidermal papillae and the shape of central vascular system. Results have indicated that detailed studies of papillae structure and types of trichomes might bring additional relevant information for the better understanding of the relations within Decaloba and other subgenera.

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“…Simulation of action potential propagation in plants depends on different factors such as explant, plant regulator growth and ambient condition. (Gloria et al 2008). Micropropagation of Canavalia rosea (Sw.) DC.…”

Section: Introductionmentioning

confidence: 99%

Baybean ( Canavalia rosea (Sw.) DC.); organogenesis, morphological and anatomical studies

et al. 2017

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Anatomical Studies of in Vitro Organogenesis Induced in Leaf-Derived Explants of Passionfruit

Cited by 27 publications

References 18 publications

Somatic embryogenesis of a wild passion fruit species Passiflora cincinnata Masters: histocytological and histochemical evidences

Somatic embryogenesis of a wild passion fruit species Passiflora cincinnata Masters: histocytological and histochemical evidences

Anatomia foliar de Passiflora subgênero Decaloba (Passifloraceae): implicações taxonômicas

Baybean ( Canavalia rosea (Sw.) DC.); organogenesis, morphological and anatomical studies

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