Suscetibilidade de Spodoptera Frugiperda a isolados geográficos de um vírus de poliedrose nuclear

Gomez, S. A.; Moscardi, F.; Sosa–Gómez, Daniel Ricardo

doi:10.1590/s0100-204x1999000900003

Cited by 4 publications

(2 citation statements)

References 14 publications

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“…Five virus isolates (Table 1) were purified by sucrose gradient centrifugation (Caballero et al 1992), and multiplied in 112 third instar H. armigera larvae fed 1.8 × 1.8 cm corn leaf discs impregnated with baculovirus suspensions at a dosage of 1 × 10 9 polyhedra per mL per isolate. The dead insects were macerated in 100 mL distilled water and strained using a thin layer of cotton (Gomez et al 1999). The polyhedra obtained from this step were used in the bioassays.…”

Section: Methodsmentioning

confidence: 99%

Genetic Identification and Biological Characterization of Baculovirus Isolated from Helicoverpa armigera (Lepidoptera: Noctuidae) in Brazil

Costa

Soares

Dimaté

et al. 2019

Florida Entomologist

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Section: Methodsmentioning

confidence: 99%

Genetic Identification and Biological Characterization of Baculovirus Isolated from Helicoverpa armigera (Lepidoptera: Noctuidae) in Brazil

Costa

Soares

Dimaté

et al. 2019

Florida Entomologist

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“…Para tanto, os dados de sobrevivência dos três bioensaios foram anotados periodicamente e utilizados para calcular a mortalidade de forma acumulada, com base no total de indivíduos mortos; em seguida, a mortalidade foi submetida à análise de regressão linear para determinar o tempo letal (Gomez et al, 1999). Para a análise de regressão, o tempo foi transformado em log 10 , e a percentagem de mortalidade foi usada diretamente e transformada em probit.…”

Section: Methodsunclassified

Índice de adaptação e tempo letal da lagarta-do-cartucho em milho Bt

Waquil

Pereira

Carvalho

et al. 2016

Pesq. agropec. bras.

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Resumo -O objetivo deste trabalho foi avaliar o índice de adaptação e o tempo letal de populações da lagarta-do-cartucho [Spodoptera frugiperda (Lepidoptera: Noctuidae)] em milho Bt que expressa as proteínas Cry1Ab, Cry1F, Cry1A.105/Cry2Ab2 e Vip3Aa20. Três bioensaios foram conduzidos com lagartas coletadas em Inhaúma, MG, e em Sinop, MT. As lagartas dessas populações foram alimentadas com folhas de milho não Bt e Bt. As seguintes variáveis foram avaliadas: sobrevivência larval, biomassa de pupas e período de desenvolvimento larval. Em seguida, foram calculados os índices de adaptação e o tempo letal dessas populações. Para a análise do tempo letal, também foram utilizados dados de mortalidade de uma população obtida de milho Bt Cry1F em Piumhi, MG. O milho Bt Cry1Ab apresentou eficiência limitada, mas duradoura. Já os milhos Bt Cry1A.105/Cry2Ab2 e Vip3Aa20 reduziram o desempenho de S. frugiperda, respectivamente, a menos de 5 e 0%, independentemente da origem da colônia. A população originada de Sinop apresentou alto desempenho larval no milho Bt Cry1F, o que mostra sua resistência a essa proteína e certo nível de custo adaptativo. O tempo letal pode ser uma variável indicadora de evolução de resistência, pois é maior para as populações com maior adaptação.Termos para indexação: Spodoptera frugiperda, Zea mays, desempenho larval, manejo da resistência, manejo integrado de pragas, milho transgênico. Fitness index and lethal time of fall armyworm on Bt cornAbstract -The objective of this work was to evaluate the fitness index and lethal time of fall armyworm [Spodoptera frugiperda (Lepidoptera: Noctuidae)] populations on Bt corn expressing the Cry1Ab, Cry1F, Cry1A.105/Cry2Ab2, and Vip3Aa20 proteins. Three bioassays were conducted with larvae collected at the municipality of Inhaúma, in the state of Minas Gerais, and at the municipality of Sinop, in the state of Mato Grosso, Brazil. Larvae from these populations were fed with leaves of non-Bt and Bt corn. The following variables were evaluated: larval survival, pupae mass, and larval development period. Then, the fitness indexes and lethal time of these populations were calculated. For lethal time analysis, mortality data of a population collected from Cry1F Bt corn in the municipality of Piumhi, in the state of Minas Gerais, were also used. The Cry1Ab Bt corn showed a limited but durable efficiency. The Cry1A.105/Cry2Ab2 and Vip3Aa20 Bt corn reduced S. frugiperda performance, respectively, to less than 5 and 0%, regardless of the origin of the colony. The population from Sinop showed high larval performance in Cry1F Bt corn, showing its resistance to this protein and a certain level of fitness cost. The lethal time can be a variable that indicates evolution of resistance, since it is greater for populations with greater fitness.

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Group living and investment in immune defence: an interspecific analysis

Wilson

Knell

Boots

et al. 2003

Journal of Animal Ecology

123

119

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Summary 1.Since parasite transmission is often density-dependent, group living is normally thought to lead to an increased exposure to parasitism. As a consequence, it is predicted that animals living in groups will invest more resources (energy, time, risk, etc.) in parasite defence than those living solitarily. 2. We tested this prediction by measuring basal immune parameters in the larvae of 12 species of Lepidoptera, grouped into six phylogenetically matched species-pairs, each comprising one solitary feeding and one gregariously feeding species. 3. Contrary to expectation, the solitary species in all six species-pairs had higher total haemocyte counts than the gregarious species, and in five out of six species-pairs the solitary species also exhibited higher phenoloxidase activity. Both measurements were positively correlated with each other and with the magnitude of the cellular encapsulation response. 4. The relationship between infection risk and group living was investigated with a dynamic, spatially explicit, host-pathogen model. This shows that when individuals aggregate in groups, the per capita risk of infection can be reduced if the lower betweengroup transmission more than compensates for the higher within-group transmission. 5. We conclude that the expectation that group living always leads to increased exposure to pathogens and parasites is overly simplistic, and that the specific details of the social system in question will determine if there is increased or decreased exposure to infection.

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Suscetibilidade de Spodoptera Frugiperda a isolados geográficos de um vírus de poliedrose nuclear

Cited by 4 publications

References 14 publications

Genetic Identification and Biological Characterization of Baculovirus Isolated from Helicoverpa armigera (Lepidoptera: Noctuidae) in Brazil

Genetic Identification and Biological Characterization of Baculovirus Isolated from Helicoverpa armigera (Lepidoptera: Noctuidae) in Brazil

Índice de adaptação e tempo letal da lagarta-do-cartucho em milho Bt

Group living and investment in immune defence: an interspecific analysis

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