Diversidade genética e eficiência simbiótica de rizóbios noduladores de acácia-negra de solos do Rio Grande do Sul

Vargas, Luciano Kayser; Lisboa, Bruno Brito; Scholles, Dércio; Silveira, José Ricardo Pfeifer; Jung, Gabriela Cardoso; Granada, Camille Eichelberger; Neves, Andrei Gibbon; Braga, Marcos Martins; Negreiros, Tatiana Alves

doi:10.1590/s0100-06832007000400005

Cited by 18 publications

(10 citation statements)

References 16 publications

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“…Os outros 32 isolados estudados, 61 % do total, mostraram-se sensíveis a pH 4,2. Resultados semelhantes foram obtidos por Hara & Oliveira (2004) e Vargas et al (2007), em estudos com rizóbios, bem como no trabalho de Wood & Cooper (1985), nos quais esses autores relatam que estirpes de rizóbios para Lotus foram tolerantes com 20 e 50 μM de Al a pH 5,5. Entretanto, nos estudos de Räsänen & Lindström (1997), 20 μM de Al em meio de cultura inibiram a multiplicação de células de Rhizobium galegae em pH baixo.…”

Section: Resultsunclassified

Diversidade genética, tolerância aos fatores de acidez e eficiência simbiótica de rizóbios para cornichão de solos do Rio Grande do Sul

Martins¹,

Vargas²,

Lisboa³

et al. 2011

Rev. Bras. Ciênc. Solo

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O cornichão é uma leguminosa forrageira perene hiberno-primaveril de grande importância para o Rio Grande do Sul, destacando-se pela capacidade de se manter em solos relativamente ácidos e pouco férteis. Com este trabalho, objetivou-se a seleção de rizóbios para cornichão tolerantes à acidez e ao Al tóxico e eficientes na fixação biológica de N em solos de baixa fertilidade. Foram avaliados 52 isolados de rizóbios de Lotus spp. obtidos de solos de cinco localidades do Rio Grande do Sul. Os rizóbios foram avaliados quanto à diversidade genética, à tolerância a pH 4,2 e ao Al tóxico. Entre os rizóbios tolerantes a fatores de acidez, sete foram avaliados quanto à eficiência simbiótica com plantas, em casa de vegetação, em vasos com solo não estéril. Observou-se alta diversidade genética entre os rizóbios estudados, dos quais 16 foram tolerantes a pH 4,2 e a 50 µM de Al em meio de cultura, produzindo populações da ordem de 10(7) até 10(8) UFC mL-1. Os sete rizóbios testados em casa de vegetação superaram as estirpes atualmente recomendadas para a produção de inoculantes, o que demonstra a existência, em solos do Rio Grande do Sul, de rizóbios tolerantes à acidez do solo e eficientes como fixadores de N em plantas de cornichão.

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Section: Resultsunclassified

Diversidade genética, tolerância aos fatores de acidez e eficiência simbiótica de rizóbios para cornichão de solos do Rio Grande do Sul

Martins¹,

Vargas²,

Lisboa³

et al. 2011

Rev. Bras. Ciênc. Solo

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“…The presence of intermediate-growth isolates with production of alkali, characteristics that exemplify the genus Bradyrhizobium (Vargas et al 2007) suggests that this genus performs symbiosis with P. lunatus. Similar results were observed by Ormeno-Orrillo et al (2006) in isolates obtained from lima bean nodules grown in the soil of Peru, where the genus Bradyrhizobium is the predominant symbiont.…”

Section: Resultsmentioning

confidence: 99%

Genetic diversity among native isolates of rhizobia from Phaseolus lunatus

et al. 2010

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The genetic diversity among native nodular rhizobia of Lima bean in soil from Piauí State, Brazil, was characterized and evaluated. The genotype UFPI-491 was used as a trap plant for rhizobia, with soil samples collected in Piaui State being used as the inoculum source. For isolation, nodules were collected at 45 days after seedling emergence-a period that presented the highest values for number and biomass of nodules evaluated in a previous experiment. In total, 50 isolates were obtained and placed into groups based on divergence from the morphological and physiological characterization. In general, the restriction patterns obtained with endonucleases MboI, HaeIII and NheI showed sufficient variability to discriminate between the isolates in this study. The main characteristics exhibited by isolates identified species from the genera Bradyrhizobium, Mesorhizobium and Rhizobium.

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“…Vargas et al (2007) evaluated the genetic diversity of black wattle (Acacia mearnsii) nodulating rhizobia, and found no isolate with a similarity degree to the reference strains of over 73 %. In fact, the reference strains were found to be more similar to each other than to any native isolate.…”

Section: Resultsmentioning

confidence: 99%

“…A similarity tree was constructed, using software PAST, based on the Jaccard coefficient to estimate similarity among isolates. Hierarchical cluster analysis was performed using the UPGMA (Unweighted Pair Group Mathematical Average) algorithm (Vargas et al, 2007).…”

Section: Sampling and Rhizobia Isolationmentioning

confidence: 99%

Occurrence of plant growth-promoting traits in clover-nodulating rhizobia strains isolated from different soils in Rio Grande do Sul state

Vargas

Lisboa

Schlindwein

et al. 2009

Rev. Bras. Ciênc. Solo

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SUMMARYIn the last decades, the use of plant growth-promoting rhizobacteria has become an alternative to improve crop production. Rhizobium leguminosarum biovar trifolii is one of the most promising rhizobacteria and is even used with nonlegume plants. This study investigated in vitro the occurrence of plant growthpromoting characteristics in several indigenous R. leguminosarum biovar trifolii isolated from soils in the State of Rio Grande do Sul, Brazil. Isolates were obtained at 11 locations and evaluated for indoleacetic acid and siderophore production and inorganic phosphate solubilization. Ten isolates were also molecularly characterized and tested for antagonism against a phytopathogenic fungus and for plant growth promotion of rice seedlings. Of a total of 252 isolates, 59 produced indoleacetic acid, 20 produced siderophores and 107 solubilized phosphate. Some degree of antagonism against Verticillium sp. was observed in all tested isolates, reducing mycelial growth in culture broth. Isolate AGR-3 stood out for increasing root length of rice seedlings, while isolate ELD-18, besides increasing root length in comparison to the uninoculated control, also increased the germination speed index, shoot length, and seedling dry weight. These results confirm the potential of some strains of R. leguminosarum biovar trifolii as plant growth-promoting rhizobacteria.Index terms: rhizobacteria, siderophore, indoleacetic acid, phosphate solubilization, antagonism, rice.(

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Diversidade genética e eficiência simbiótica de rizóbios noduladores de acácia-negra de solos do Rio Grande do Sul

Cited by 18 publications

References 16 publications

Diversidade genética, tolerância aos fatores de acidez e eficiência simbiótica de rizóbios para cornichão de solos do Rio Grande do Sul

Diversidade genética, tolerância aos fatores de acidez e eficiência simbiótica de rizóbios para cornichão de solos do Rio Grande do Sul

Genetic diversity among native isolates of rhizobia from Phaseolus lunatus

Occurrence of plant growth-promoting traits in clover-nodulating rhizobia strains isolated from different soils in Rio Grande do Sul state

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