Ocorrência e diversidade de bactérias diazotróficas associadas a gramíneas forrageiras do Pantanal Sul Matogrossense

Brasil, Marivaine da Silva; Baldani, José Ivo; Baldani, V. L. D.

doi:10.1590/s0100-06832005000200003

Cited by 18 publications

(11 citation statements)

References 27 publications

(15 reference statements)

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“…The ability of the bacterial strains to promote plant growth was evaluated and we observed that among the eight bacterial strains evaluated, four of them were able to increase the root dry mass compared to the absolute control and the positive controls inoculated with the BR 11001 (A. brasilense) and BR 11175 (H. seropedicae) ( Table 2). Three of the isolates were also able to increase the root dry mass of O. sativa more DISCUSSION Although only around 5% of the bacterial isolates are able to fix nitrogen in micro-aerophilic conditions, some of these bacteria may not be isolated if the generally used strategy was applied to obtain the diazotrophic bacterial strains from crop species, during the first isolation step in semisolid medium (Stoltzfus et al 1997); because the semisolid culture media normally used during these isolations is indicated to obtain the most studied and characterized diazotrophic bacteria such as Azospirillum (Baldani & Döbereiner 1980); Herbaspirillum (Brasil et al 2005); Burkholderia (Guimarães et al, 2007) and Sphingomonas (Videira et al 2009).…”

Section: Growth Promoting Assaymentioning

confidence: 99%

“…Normally, the studies that evaluate the isolation and characterization of nifH + bacteria from non-legume plants indicate that the majority of the isolates are obtained from root tissues (Videira et al 2009, Vendan et al 2010, Venieraki et al 2011. However, there is evidence that during the vegetative development of grasses in plots where the water availability ranges from flood to drought, as in the Amazon, more diazotrophs must be isolated from shoots rather than from plant roots (Brasil et al 2005, Koomnok et al 2007.…”

Section: Growth Promoting Assaymentioning

confidence: 99%

“…Despite the fact that research on grasses and diazotrophic bacteria association has increased, there is a lack of knowledge regarding these associations for wild plant species in Brazil. Few recent studies have shown large diversity of cultivated bacteria isolated from wild grasses in Brazil (Brasil et al 2005, 2012.…”

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confidence: 99%

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Diazotrophic bacteria isolated from wild rice <i>Oryza glumaepatula</i> (Poaceae) in the Brazilian Amazon

Fernandes‐Júnior

Pereira

Perin³

et al. 2013

RBT

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Bacterias diazotróficas aisladas de arroz silvestre Oryza glumaepatula (Poaceae) en la Amazonia brasileña. The association of wild grasses with diazotrophic bacteria in Brazilian biomes is poorly understood. The isolation and characterization of bacteria associated with wild grasses can contribute to understand the diazotrophic ecology as well as to identify bacteria with biotechnological applications. In this study, we isolated and characterized diazotrophic bacterial isolates from Oryza glumaepatula collected in Cerrado and Forest areas of the Amazon in Roraima State, Brazil. Healthy O. glumepatula plants were collected at five sampling sites at Forest and seven at Cerrado, respectively. The plants were collected at the Cerrado areas in September 2008 while the Forest plants were collected in June/2008 and April/2009. The plants and the soil adhering to the roots were transferred to pots and grown for 35 days in greenhouse conditions. During the harvest, the shoots and the roots were crushed separately in a saline solution; the suspension was diluted serially and inoculated in Petri dishes containing Dyg's medium. All distinct bacterial colonies were purified in the same medium. The diazotrophic capacity of each bacterium in microaerophilic conditions was assessed in semisolid BMGM medium. In addition, the pellicles forming bacterial isolates were also evaluated by PCR amplification for nifH gene. The diversity of nifH + bacteria was analyzed by Box-PCR fingerprinting. For selected strains, the growth promoting capacity of O. sativa as a model plant was also evaluated. A total of 992 bacterial isolates were obtained. Fiftyone bacteria were able to form pellicles in the semisolid medium and 38 also positively amplified the 360bp nifH gene fragment. Among the 38 nifH + isolates, 24 were obtained from the shoots, while 14 originated from the roots. The Box-PCR profiles showed that the bacterial isolates obtained in this study presented a low similarity with the reference strains belonging to the Herbaspirillum, Azospirillum and Burkholderia genus. The growthpromoting ability was confirmed for at least five isolates. For these bacteria, the root and shoot growing results showed higher increases when compared to those observed in plants inoculated with the evaluated reference strains. These results indicate that O. glumaepatula is colonized by a high diverse diazotrophic community in

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Section: Growth Promoting Assaymentioning

confidence: 99%

Section: Growth Promoting Assaymentioning

confidence: 99%

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Diazotrophic bacteria isolated from wild rice <i>Oryza glumaepatula</i> (Poaceae) in the Brazilian Amazon

Fernandes‐Júnior

Pereira

Perin³

et al. 2013

RBT

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“…literature [35,36], but the management of cultivation of these grasses and the niche of colonization can alter this community [12,30]. Therefore, due to the capacity to colonize intra-and intercellular spaces, the endophytic microorganisms have a close relationship with their host [10], which contributed to reducing their diversity.…”

Section: Degradation Stage and Association Regionmentioning

confidence: 99%

Diazotrophic Bacterial Community of Degraded Pastures

Oliveira

Figueredo

Diniz

et al. 2017

Applied and Environmental Soil Science

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Pasture degradation can cause changes in diazotrophic bacterial communities. Thus, this study aimed to evaluate the culturable and total diazotrophic bacterial community, associated with regions of the rhizosphere and roots of Brachiaria decumbens Stapf. pastures in different stages of degradation. Samples of roots and rhizospheric soil were collected from slightly, partially, and highly degraded pastures. McCrady's table was used to obtain the Most Probable Number (MPN) of bacteria per gram of sample, in order to determine population density and calculate the Shannon-Weaver diversity index. The diversity of total diazotrophic bacterial community was determined by the technique of Denaturing Gradient Gel Electrophoresis (DGGE) of the nif H gene, while the diversity of the culturable diazotrophic bacteria was determined by the Polymerase Chain Reaction (BOX-PCR) technique. The increase in the degradation stage of the B. decumbens Stapf. pasture did not reduce the population density of the cultivated diazotrophic bacterial community, suggesting that the degradation at any degree of severity was highly harmful to the bacteria. The structure of the total diazotrophic bacterial community associated with B. decumbens Stapf. was altered by the pasture degradation stage, suggesting a high adaptive capacity of the bacteria to altered environments.

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“…The phytophysiognomies of these provinces are mixed in the Pantanal wetlands, in floodable and flood-free areas, in edge areas, and in the plateaus (Scremin-Dias et al, 2011). Accordingly, evaluations of the diazotrophic bacteria associated with native species from the Pantanal have indicated the presence of a highly diverse and efficient diazotrophic community (James et al, 2001;Brasil et al, 2005), and that the flood regime influences the diversity of plant-associated diazotrophic bacteria (James et al, 2001).…”

Section: Introductionmentioning

confidence: 99%

Phenotypic and molecular fingerprinting of fast growing rhizobia of field-grown pigeonpea from the eastern edge of the Brazilian Pantanal

Costa¹,

Schiavo²,

Brasil³

et al. 2014

Genet. Mol. Res.

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ABSTRACT. The aim of this study was to evaluate the diversity of rhizobial isolates obtained from root nodules of pigeonpea plants grown at the eastern edge of the Brazilian Pantanal. The bacterial isolates were isolated from root nodules from field-growing pigeonpea grown in two rural settlements of the Aquidauana municipality. The bacterial isolates were characterized phenotypically by means of cultural characterization, intrinsic antibiotic resistance (IAR), salt and high incubation temperature tolerance, and amylolytic and cellulolytic activities. The molecular characterization of the bacterial isolates was carried out using amplified ribosomal DNA restriction analysis (ARDRA) and Box-polymerase chain reaction (PCR) techniques. In addition, the symbiotic performance of selected rhizobial isolates was evaluated in a greenhouse experiment using sterile substrate. The phenotypic characterization revealed that the bacterial strains obtained from pigeonpea root nodules presented characteristics that are uncommon among rhizobial isolates, indicating the presence of new species nodulating the pigeonpea plants in the Brazilian Pantanal. The molecular fingerprinting of these bacterial isolates also showed a highly diverse collection, with both techniques revealing less than 25% similarity among bacterial isolates. The evaluation of symbiotic performance also indicated the presence of microorganisms with high potential to increase the growth and nitrogen content at the shoots of pigeonpea plants. The results obtained in this study indicate the presence of a highly diversified rhizobial community nodulating the pigeonpea at the eastern edge of the Brazilian Pantanal.

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Ocorrência e diversidade de bactérias diazotróficas associadas a gramíneas forrageiras do Pantanal Sul Matogrossense

Cited by 18 publications

References 27 publications

Diazotrophic bacteria isolated from wild rice <i>Oryza glumaepatula</i> (Poaceae) in the Brazilian Amazon

Diazotrophic bacteria isolated from wild rice <i>Oryza glumaepatula</i> (Poaceae) in the Brazilian Amazon

Diazotrophic Bacterial Community of Degraded Pastures

Phenotypic and molecular fingerprinting of fast growing rhizobia of field-grown pigeonpea from the eastern edge of the Brazilian Pantanal

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