2014
DOI: 10.4238/2014.january.21.16
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Abstract: ABSTRACT. The aim of this study was to evaluate the diversity of rhizobial isolates obtained from root nodules of pigeonpea plants grown at the eastern edge of the Brazilian Pantanal. The bacterial isolates were isolated from root nodules from field-growing pigeonpea grown in two rural settlements of the Aquidauana municipality. The bacterial isolates were characterized phenotypically by means of cultural characterization, intrinsic antibiotic resistance (IAR), salt and high incubation temperature tolerance, a… Show more

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Cited by 7 publications
(2 citation statements)
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“…Among the cultivated species, studies conducted in Brazil have already examined the genetic variability of rhizobia of legumes such as pigeonpea (Costa et al, 2014), peanuts (Lyra et al, 2013;TorresJúnior et al, 2014), common beans (Stocco et al, 2008), among others. This technique is also used to examine the variability of strains isolated from native plants such as bromeliad (Giongo et al, 2013), wild rice (Fernandes Júnior et al, 2013) and legumes (Granada et al, 2014).…”
Section: Discussionmentioning
confidence: 99%
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“…Among the cultivated species, studies conducted in Brazil have already examined the genetic variability of rhizobia of legumes such as pigeonpea (Costa et al, 2014), peanuts (Lyra et al, 2013;TorresJúnior et al, 2014), common beans (Stocco et al, 2008), among others. This technique is also used to examine the variability of strains isolated from native plants such as bromeliad (Giongo et al, 2013), wild rice (Fernandes Júnior et al, 2013) and legumes (Granada et al, 2014).…”
Section: Discussionmentioning
confidence: 99%
“…Phenotypic characterization is the basis of polyphasic taxonomy and, together with molecular techniques, has contributed to an improved understanding of the physiology and genetic diversity of rhizobia (Zerhari et al, 2000;Fall et al, 2008;Rasul et al, 2012). Among the molecular characterization techniques, fingerprinting by means of Box-PCR generated profiles have been used to examine the diversity of diazotrophic bacteria associated with the (Stocco et al, 2008;Costa et al, 2014;Torres-Júnior et al, 2014) and native species (Fernandes Júnior et al, 2013;Granada et al, 2014) from Brazil. This simple and low-cost technique allows the discrimination among several rhizobial isolates and reference strains, making possible a fast fingerprinting and selection of bacterial isolates.…”
Section: Introductionmentioning
confidence: 99%