Clonal multidrug-resistant Corynebacterium striatum within a nosocomial environment, Rio de Janeiro, Brazil

Identification of clinically relevant Corynebacterium strains by Api Coryne, MALDI-TOF-mass spectrometry and molecular approaches

“…Polymerase chain reaction (PCR) was carried out in a final volume of 50 ml as described previously [1,17] using the primers C2700F and C3130R and universal oligonucleotides for the amplification of partial rpoB gene 16S rRNA gene respectively. PCR-amplified fragments sequencing on both stands was performed as previously described [18,19] by a 3500 D X Genetic Analyzer Applied Biosystem sequencer. Obtained sequences were aligned and interpreted via the program BLAST of National Center BioInformatic (NCBI http://blast.ncbi.nlm.nih.gov/) and the program BioInformatic Bacteria Identification (BIBI http:// umr5558-sud-str1.univ-lyon1.fr/lebibi/lebibi.cgi).…”

“…rpoB based identification to the species level required 96.6% sequence identity with 2% separation between species. 16S rRNA gene based identification to the species level required 99% identity and 0.8% separation between species [12,17,18]. For C. diphtheriae strain, the toxA gene was detected by classical PCR, as previously described [9].…”

Characteristics of Multidrug-Resistant <i>Corynebacterium</i> spp. Isolated from Blood Cultures of Hospitalized Patients in Japan

“…Recently, Corynebacterium spp. was also reported to cause several nosocomial outbreaks (8)(9)(10), and its pathogenic potential has been proven, particularly in the setting of immunosuppression and medical devices (11)(12)(13)(14). Drugresistant Corynebacterium spp.…”

Cellulitis and Bacteremia due to <i>Corynebacterium striatum</i> Identified by Matrix-assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry

“…remains difficult, it may become easier due to the increasing use of MALDI-TOF MS. As a result, the number of cases of cellulitis determined to be due to C. striatum may increase. Because multidrug-resistant, including ampicillin-resistant, strains of C. striatum have been reported in many countries since 2003 (7,(25)(26)(27), antimicrobial susceptibility testing such as the SHS method should be performed.…”