Characteristics of Multidrug-Resistant &lt;i&gt;Corynebacterium&lt;/i&gt; spp. Isolated from Blood Cultures of Hospitalized Patients in Japan

Qin, Liang; Sakai, Yoshiro; Bao, Rong; Xie, Hongwei; Masunaga, Kenji; Miura, Miho; Hashimoto, Kouji; Tanamachi, Chiyoko; Hu, Bijie; Watanabe, Hiroshi

doi:10.7883/yoken.jjid.2015.530

Cited by 20 publications

(29 citation statements)

References 35 publications

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“…Only a single isolate of C. striatum (1/931 isolates) and none of the C. jeikeium (n ϭ 76) or C. resistens (n ϭ 19) isolates were susceptible to penicillin. Susceptibility to penicillin and other beta-lactams has reduced in the last 2 decades among certain nondiphtherial corynebacteria, including C. striatum (7)(8)(9)(10). Consistent with our findings, a study from Japan showed that none of the C. striatum (n ϭ 22) isolates recovered from blood specimens were susceptible to penicillin (9).…”

supporting

confidence: 90%

“…C. minutissimum was previously considered to be susceptible to many different classes of antibiotics (1,7,9,26), but most of the isolates in this study were resistant to more than four drug classes, including penicillin, erythromycin, gentamicin, and ciprofloxacin. Similarly, C. striatum was found to be highly multidrug resistant, with varied percentages of resistance to penicillin, erythromycin, gentamicin, ciprofloxacin, and daptomycin.…”

mentioning

confidence: 81%

“…Susceptibility to penicillin and other beta-lactams has reduced in the last 2 decades among certain nondiphtherial corynebacteria, including C. striatum (7)(8)(9)(10). Consistent with our findings, a study from Japan showed that none of the C. striatum (n ϭ 22) isolates recovered from blood specimens were susceptible to penicillin (9). However, a recent Canadian study (4) reported the susceptibility rate of penicillin for the Corynebacterium genus to be 77%, which is much higher than the susceptibility rate (14.5%) found in our study.…”

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confidence: 99%

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In VitroAntibiotic Susceptibility Pattern of Non-diphtheriae Corynebacterium Isolates in Ontario, Canada, from 2011 to 2016

Neemuchwala

Soares

Ravirajan

et al. 2018

Antimicrob Agents Chemother

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Non--associated disease has been increasingly observed and often presents a conundrum to the treating physician. Analysis of antibiotic susceptibility testing data for 1,970 clinical isolates received between 2011 and 2016 revealed that empirical drug treatment options are limited to vancomycin and linezolid. was the most frequently observed species during this study period, along with and Low levels of susceptibility to penicillin (14.5%), erythromycin (15.1%), and clindamycin (8.7%) were observed for non- species, while 3.0% of isolates were not susceptible to daptomycin. Similarly, 26.9% and 38.1% of isolates were susceptible to ciprofloxacin and trimethoprim-sulfamethoxazole, respectively. Our data show much lower susceptibility to penicillin than previously reported in the literature and an increasing number of isolates resistant to daptomycin, highlighting the need for continued antibiotic surveillance studies for appropriate patient management and treatment success.

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confidence: 90%

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confidence: 81%

mentioning

confidence: 99%

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In VitroAntibiotic Susceptibility Pattern of Non-diphtheriae Corynebacterium Isolates in Ontario, Canada, from 2011 to 2016

Neemuchwala

Soares

Ravirajan

et al. 2018

Antimicrob Agents Chemother

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“…These bacteria are members of the microbiota of the skin and mucous membranes (Qin et al 2017;Santos et al 2017;Kang et al 2018). The potential association between coryneforms and clinical infections can be attributed to immunosuppression, severe underlying medical disorders or invasive procedures (Nhan et al 2012;Cacopardo et al 2013;Kimura et al 2017;Qin et al 2017). In order to shed more light on the role of diptheroids as medically relevant microorganisms, their inherent low virulence should be confronted to the increasingly reported multidrug resistance (Yoon et al 2011;Bernard 2012b;Kimura et al 2017;Qin et al 2017), and the ability to adhere to biotic and abiotic surfaces and/or to form biofilms (Kwaszewska et al 2006;Souza et al 2015a;Souza et al 2015b;Qin et al 2017;Kang et al 2018).…”

Section: Introductionmentioning

confidence: 99%

Cytokine Levels in the In Vitro Response of T Cells to Planktonic and Biofilm Corynebacterium amycolatum

Olender

Bogut

Magryś

et al. 2019

Polish Journal of Microbiology

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Unravelling of the interplay between the immune system and non-diphtheria corynebacteria would contribute to understanding their increasing role as medically important microorganisms. We aimed at the analysis of pro- (TNF, IL-1β, IL-6, IL-8, and IL-12p70) and anti-inflammatory (IL-10) cytokines produced by Jurkat T cells in response to planktonic and biofilm Corynebacterium amycolatum. Two reference strains: C. amycolatum ATCC 700207 (R-CA), Staphylococcus aureus ATCC 25923 (R-SA), and ten clinical strains of C. amycolatum (C-CA) were used in the study. Jurkat T cells were stimulated in vitro by the planktonic-conditioned medium (PCM) and biofilm-conditioned medium (BCM) derived from the relevant cultures of the strains tested. The cytokine concentrations were determined in the cell culture supernatants using the flow cytometry. The levels of the cytokines analyzed were lower after stimulation with the BCM when compared to the PCM derived from the cultures of C-CA; statistical significance (p < 0.05) was observed for IL-1β, IL-12 p70, and IL-10. Similarly, planktonic R-CA and R-SA stimulated a higher cytokine production than their biofilm counterparts. The highest levels of pro-inflammatory IL-8, IL-1β, and IL-12p70 were observed after stimulation with planktonic R-SA whereas the strongest stimulation of anti-inflammatory IL-10 was noted for the BCM derived from the mixed culture of both reference species. Our results are indicative of weaker immunostimulatory properties of the biofilm C. amycolatum compared to its planktonic form. It may play a role in the persistence of biofilm-related infections. The extent of the cytokine response can be dependent on the inherent virulence of the infecting microorganism.

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“…These infections typically involve immunocompromised patients and are associated with circumstances favoring bacterial translocation from the mucous membranes or the skin through catheters and intubation or resulting from treatment that lead to disruption of tissue integrity. An important feature of Corynebacterium species, which promotes infections, is their ability to produce a biofilm on artificial surfaces (Mattos-Guaraldi and Formiga 1991; Kwaszewska et al 2006;Sousa et al 2011;Gomes et al 2013;de Souza et al 2015;Leal et al 2016, Qin et al 2017Kang et al 2018). When it occurs, the intravenous biomaterial's surface colonization may lead to a bloodstream infection (Martínez-Martínez et al 1995;Moore and Norton 1995;Campanile et al 2009), urinary tract infections (Soriano et al 2009), infections associated with the implant surface (Merhej et al 2009) or as the result of intubation can cause respiratory tract infections (Williams et al 2012;Wojewoda et al 2012).…”

Section: Introductionmentioning

confidence: 99%

A Novel Approach to Study the Effect of Ciprofloxacin on Biofilms of Corynebacterium spp. Using Confocal Laser Scanning Microscopy

Olender

Bogut

Magryś

et al. 2018

Polish Journal of Microbiology

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Non-diphtherial corynebacteria are Gram-positive rods that cause opportunistic infections, what is supported by their ability to produce biofilm on artificial surfaces. In this study, the characteristic of the biofilm produced on vascular and urological catheters was determined using a confocal microscopy for the most frequently involved in infections diphtheroid species. They were represented by the reference strains of Corynebacterium striatum ATCC 6940 and C. amycolatum ATCC 700207. The effect of ciprofloxacin on the biofilm produced by the antibiotic-susceptible C. striatum strain was evaluated using three concentrations of the antimicrobial agent (2 ×, 4 ×, and 6 × the MIC – the Minimum Inhibitory Concentration). The basis for the interpretation of results was the statistical analysis of maximum points readings from the surface comprising a total of 245 areas of the biofilm image under the confocal microscope. It was observed that ciprofloxacin at a concentration equal to 4 × MIC paradoxically caused an enlargement of areas with live bacteria within the biofilm. Biofilm destruction required the application of ciprofloxacin at a concentration higher than 6 × MIC. This suggests that the use of relatively low doses of antimicrobial agents may increase the number of live bacteria within the biofilm, and further facilitate their detachment from the biofilm’s structure thus leading to the spread of bacteria into the bloodstream or to the neighboring tissues. The method of biofilm analysis presented here provides the original and novel approach to the investigation of the diphtheroid biofilms and their interaction with antimicrobial agents.

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Characteristics of Multidrug-Resistant <i>Corynebacterium</i> spp. Isolated from Blood Cultures of Hospitalized Patients in Japan

Cited by 20 publications

References 35 publications

In VitroAntibiotic Susceptibility Pattern of Non-diphtheriae Corynebacterium Isolates in Ontario, Canada, from 2011 to 2016

In VitroAntibiotic Susceptibility Pattern of Non-diphtheriae Corynebacterium Isolates in Ontario, Canada, from 2011 to 2016

Cytokine Levels in the In Vitro Response of T Cells to Planktonic and Biofilm Corynebacterium amycolatum

A Novel Approach to Study the Effect of Ciprofloxacin on Biofilms of Corynebacterium spp. Using Confocal Laser Scanning Microscopy

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