Coxiella burnetii, the agent of Q fever in Brazil: its hidden role in seronegative arthritis and the importance of molecular diagnosis based on the repetitive element IS1111 associated with the transposase gene

Rozental, Tatiana; Mascarenhas, Luis Filipe; Rozenbaum, Ronaldo; Gomes, Raphael; Mattos, Grasiely Souza; Magno, Cecília Carlos; Almeida, D.; Rossi, Maria Inês Doria; Favacho, Alexsandra Rodrigues de Mendonça; Lemos, Elba Regina Sampaio de

doi:10.1590/s0074-02762012000500021

Cited by 18 publications

(24 citation statements)

References 21 publications

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“…The serum samples were evaluated for anti-C. burnetii antibodies, phases I and II, using a commercial IFA for the detection of IgG (SCIMEDX TM , Denville, New Jersey, USA) with a cut-off titer of 64. Blood, milk, vaginal and anal swabs and fecal specimens were evaluated by PCR assay as previously described, using primers targeting the repetitive transposon-like region of C. burnetiiTrans1 (5'TATGTATCCACCGTA GCCAGTC-3') and Trans2 (5'-CCCAACAACACCTCCTTATTC-3') -and producing an expected amplifi cation product of 687 bp 8,9 . DNA was extracted from blood samples from all animals by a QIAamp DNA Blood Mini Kit (QIAGEN, Valencia, CA, USA) in accordance with the manufacturer's instructions.…”

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confidence: 99%

Molecular identification of the agent of Q fever – Coxiella burnetii – in domestic animals in State of Rio de Janeiro, Brazil

Mares-Guia

Rozental

Guterres

et al. 2014

Rev. Soc. Bras. Med. Trop.

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Introduction:Over the last recent years, the number of Q fever cases have has increased throughout the world. An epidemiological investigation was performed in the area in which the fi rst molecular documentation of Q fever in Brazil was previously reported. Methods: Indirect immunofl uorescence assay (IFA) and PCR of Coxiella burnetii targeting the htpAB gene were performed in samples from 14 dogs (blood); 1 cat (blood); 10 goats (blood, milk, vaginal swab and anal swab); 3 sheep (blood); and 2 horses (blood). Results: Two dogs, two sheep and fi ve goats were seroreactive. DNA was amplifi ed from 6 milk and 2 blood samples from goats and from dogs, respectively. The sequence of the amplicons exhibited 99% sequence similarity with the homologous sequence of the htpAB gene of C. burnetii RSA 331 (GenBank -CP000890). Conclusions: The results confi rm C. burnetii infection in animals in Rio de Janeiro and reinforce the need for the surveillance of Q fever in Brazil.

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confidence: 99%

Molecular identification of the agent of Q fever – Coxiella burnetii – in domestic animals in State of Rio de Janeiro, Brazil

Mares-Guia

Rozental

Guterres

et al. 2014

Rev. Soc. Bras. Med. Trop.

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“…Hoje se sabe, baseado em investigações filogenéticas do RNAr 16S, que essa bactéria pertence à ordem das Legionellales, grupo gama das Proteobactérias. Trata-se de um cocobacilo gram-negativo, intracelular obrigatório e de alta resistência 18,21 .…”

Section: Agente Etiológicounclassified

Coxiella burnetii e a febre Q no Brasil, uma questão de saúde pública

Damasceno

Guerra

2018

Ciênc. saúde coletiva

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Resumo A Febre Q é uma zoonose de ampla distribuição mundial, apesar dos poucos relatos associados a sua ocorrência no Brasil. “Coxiella burnetii”, agente etiológico da Febre Q, é um cocobacilo gram-negativo, parasita intracelular obrigatório da ordem Legionellales. O microrganismo geralmente está presente na urina e fezes de animais infectados, podendo ser encontrado em grande quantidade nos restos placentários de animais nascidos a termo ou produtos de aborto. A inalação de células bacterianas suspensas no ar ou aerossóis contaminados é a forma mais comum de entrar em contato com a bactéria. A febre Q é uma doença autolimitada e, geralmente, evolui de forma benigna. Nos casos onde a doença evolui de forma crônica, a endocardite é a manifestação mais frequente. O diagnóstico clínico é difícil, visto que os sintomas assemelham-se a várias outras doenças. Nos casos confirmados a antibioticoterapia é o tratamento indicado. Diante da sintomatologia pouco específica e dificuldade de diagnóstico, acredita-se que no Brasil a doença seja mais comum do que se pensa.

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“…Molecular detection of C. burnetii in Brazil was first achieved in 2011. 11 In recent years, cases of C. burnetii pneumonia 12 and endocarditis, [13][14][15] and serologic evidence of Q fever in patients infected with human immunodeficiency virus in Brazil have also been reported. 16 Published data are scarce for the remaining countries in South America and Central America; often only one reference is found in the published literature.…”

Section: Peculiar Epidemiologic Profile In Southmentioning

confidence: 99%

Q Fever in French Guiana

Eldin¹,

Mahamat²,

Demar³

et al. 2014

The American Journal of Tropical Medicine and Hygiene

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Abstract. Coxiella burnetii, the causative agent of Q fever, is present worldwide. Recent studies have shown that this bacterium is an emerging pathogen in French Guiana and has a high prevalence (24% of community-acquired pneumonia). In this review, we focus on the peculiar epidemiology of Q fever in French Guiana. We place it in the context of the epidemiology of the disease in the surrounding countries of South America. We also review the clinical features of Q fever in this region, which has severe initial presentation but low mortality rates. These characteristics seem to be linked to a unique genotype (genotype 17). Finally, we discuss the issue of the animal reservoir of C. burnetii in French Guiana, which is still unknown. Further studies are necessary to identify this reservoir. Identification of this reservoir will improve the understanding of the Q fever epidemic in French Guiana and will provide new tools to control this public health problem.

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Coxiella burnetii, the agent of Q fever in Brazil: its hidden role in seronegative arthritis and the importance of molecular diagnosis based on the repetitive element IS1111 associated with the transposase gene

Cited by 18 publications

References 21 publications

Molecular identification of the agent of Q fever – Coxiella burnetii – in domestic animals in State of Rio de Janeiro, Brazil

Molecular identification of the agent of Q fever – Coxiella burnetii – in domestic animals in State of Rio de Janeiro, Brazil

Coxiella burnetii e a febre Q no Brasil, uma questão de saúde pública

Q Fever in French Guiana

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