Production of TNF-α, nitric oxide and hydrogen peroxide by macrophages from mice with paracoccidioidomycosis that were fed a linseed oil-enriched diet

Sargi, Sheisa Cyléia; Dalalio, Márcia Machado de Oliveira; Visentainer, Jeane Eliete Laguila; Bezerra, Rafael Campos; Perini, João Angelo Lima; Stevanato, Flávia Braidotti

doi:10.1590/s0074-02762012000300003

Cited by 9 publications

(7 citation statements)

References 26 publications

(32 reference statements)

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“…In turn, TNF-α can stimulate cytokine secretion, increase the expression of adhesion molecules as well as activate neutrophils2324. TNF-α has been shown to be relevant for the physiopathology of various inflammatory conditions like rheumatic fever, rheumatoid arthritis, and RHD25.…”

Section: Discussionmentioning

confidence: 99%

Tumor necrosis factor-alpha 308G>A polymorphism and risk of rheumatic heart disease: a meta-analysis

Zheng

Zhang

Jiang

2014

Sci Rep

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Rheumatic heart disease (RHD) remains a serious cardiovascular disorder across the world. Tumor necrosis factor alpha (TNF-α) codifies a potent immunomodulator and pro-inflammatory cytokine that mediates diverse pathological processes. A promoter 308G>A polymorphism in TNF-α has been implicated in RHD risk. However, the results remain controversial. Therefore, to evaluate more precise estimations of the relationship, a meta-analysis was performed. A total of 7 studies including 735 RHD cases and 926 controls were involved in this meta-analysis. Overall, our results revealed that there was a significant association with RHD risk in three genetic models (homozygous model: OR = 3.06, 95%CI = 1.22–10.60, P = 0.020; dominant model, OR = 2.03, 95%CI = 1.01–4.07, P = 0.048; and recessive model, OR = 4.26, 95%CI = 2.41–7.55, P < 0.001). Further ethnic population analysis found a significantly increased risk of RHD among Asians and Europeans. Interestingly, similar results were found among hospital-based studies. Begg's funnel plot and Egger's test did not reveal any publication bias. Taken together, this meta-analysis demonstrates that the TNF-α 308G>A polymorphism is associated with RHD susceptibility, and it contributes to the increased risk of RHD. However, additional well-designed studies with larger samples are warranted to confirm these findings.

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Section: Discussionmentioning

confidence: 99%

Tumor necrosis factor-alpha 308G>A polymorphism and risk of rheumatic heart disease: a meta-analysis

Zheng

Zhang

Jiang

2014

Sci Rep

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“…These data suggest that these levels may have been influenced by n-3 PUFA, especially during the later phase of the disease, because eicosanoids, EPA, and DHA can regulate the expression of enzymes that are involved in the production of NO [32, 33]. An increase in NO production also suggests that the host is attempting to defend itself against the fungal infection [14]. NO production by the PGI group was reflected by macrophage fungicide activity since there was a reduced number of CFUs recovered from the culture supernatant during the later phase of the disease.…”

Section: Discussionmentioning

confidence: 99%

“…The disease is caused by the dimorphic fungus Paracoccidioides brasiliensis (Pb) and clinical forms of the disease range from asymptomatic pulmonary lesions to a systemic generalized infection, depending on the balance between fungal virulence and the host response [11, 12]. Many immunological mechanisms are triggered to combat the replication of the fungus, but the main mechanisms involve macrophages, which are the first line of defense against fungal infection [13, 14]. …”

Section: Introductionmentioning

confidence: 99%

Role of Omega-3 Polyunsaturated Fatty Acids in the Production of Prostaglandin E₂and Nitric Oxide during Experimental Murine Paracoccidioidomycosis

Sargi

Dalálio

Moraes

et al. 2013

BioMed Research International

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There has recently been increased interest in the potential health effects of omega-3 polyunsaturated fatty acids on the immune system. Paracoccidioidomycosis is the most important endemic mycosis in Latin America. Macrophages have a fundamental role and act as first line of organism defense. The purpose of this study was to analyze the effect of n-3 fatty acids on the production of PGE2 and NO by mice infected with Pb18 and fed a diet enriched with LNA for 8 weeks. To study the effect of omega-3 fatty acids on macrophage activity during experimental paracoccidioidomycosis, mice were infected with Pb18 and fed a diet supplemented with LNA. PGE2 in the serum of animals was analyzed and NO in the supernatants of macrophages cultured and challenged in vitro with Pb18 was measured. Omega-3 fatty acids seemed to decrease the production of PGE2 in vivo in the infected group fed an LNA-supplemented diet during the 4th and 8th weeks of the experiment. At the same time, we observed an increase in synthesis of NO by peritoneal macrophages in this group. Omega-3 fatty acids thus appear to have an immunomodulatory effect in paracoccidioidomycosis.

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“…PGE 2 is also able to inhibit the production of tumor necrosis factor alpha and members of the interleukin-1 family. Moreover, LNA is metabolized to EPA and DHA, which are precursors of inflammatory eicosanoids such as PGE 3 and PGF 3 that cause less inflammatory activities than PGE 2 (Perini et al, 2010;Andrade & Carmo, 2006;Garofolo & Petrilli, 2006;Sargi et al, 2012).…”

Section: Introductionmentioning

confidence: 99%

<b>Influence of n-3 Polyunsaturated Fatty Acid in the Proliferative Activity of Lymphocytes During Experimental Infection with <i>Paracoccidioides brasiliensis

Navarini

Sargi²,

Visentainer

et al. 2018

Acta Sci. Health Sci.

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ABSTRACT. It has been shown that diets containing polyunsaturated omega-3 fatty acids may modulate the immune system. Paracoccidioidomycosis (PCM) is a systemic mycosis of great importance in Latin America, caused by the thermo-dimorphic fungus Paracoccidioides brasiliensis. The aim of this study was to evaluate the influence of n-3 PUFAs in lymphoproliferative activity in murine experimental PCM. Spleen lymphocytes from animals infected with an isolate of P. brasiliensis (Pb18) and fed with diets supplemented with flaxseed oil or a commercial diet for eight weeks, were obtained and cultured for evaluation of lymphoproliferative activity by MTT assay. In uninfected animals and those fed an enriched-flaxseed diet the evaluated lymphoproliferative activity was inhibited in the first week; however, this activity resumed at later stages. On the other hand, in animals infected and fed an enriched-flaxseed diet, the interaction between the stimulus given by the presence of the fungus and the action of n-3 PUFAs appeared to stimulate lymphocyte proliferation after the first week of infection. n-3 PUFAs are shown to influence lymphoproliferative activity of mice in experimental PCM.Keywords: omega-3, paracoccidioidomycosis, imune cell.Influência do ácido graxo poli-insaturado n-3 na atividade proliferativa de linfócitos durante a infecção com Paracoccidioides brasiliensis RESUMO. Tem-se demonstrado que dietas contendo ácido graxo poli-insaturado ômega-3 podem modular o sistema imune. A Paracoccidioidomicose é uma micose sistêmica de grande importância na América Latina, causada pelo fungo termo-dimórfico Paracoccidioides brasiliensis. O objetivo do estudo foi avaliar a influência do AGPI n-3 na atividade linfoproliferativa na PCM experimental murina. Linfócitos esplênicos de animais infectados com o isolado de P. brasiliensis e alimentados com uma dieta suplementada com óleo de linhaça por 8 semanas, foram obtidos e cultivados para avaliação da atividade linfoproliferativa através do ensaio de MTT. Nos animais não infectados e alimentados com a dieta enriquecida com linhaça houve a inibição da atividade linfoproliferativa na primeira semana, sendo retomada no estágio mais tardio. Por outro lado, nos animais infectados e suplementados com a dieta com óleo de linhaça, a interação entre o estímulo dado pela presença do fungo e a ação do AGPI n-3 parecem ter estimulado a proliferação de linfócitos depois da primeira semana de infecção. AGPI n-3 podem ter influenciado a atividade linfoproliferativa dos camundongos na PCM experimental.Palavras-chave: ômega-3, paracoccidioidomicose, células imune.

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Production of TNF-α, nitric oxide and hydrogen peroxide by macrophages from mice with paracoccidioidomycosis that were fed a linseed oil-enriched diet

Cited by 9 publications

References 26 publications

Tumor necrosis factor-alpha 308G>A polymorphism and risk of rheumatic heart disease: a meta-analysis

Tumor necrosis factor-alpha 308G>A polymorphism and risk of rheumatic heart disease: a meta-analysis

Role of Omega-3 Polyunsaturated Fatty Acids in the Production of Prostaglandin E₂and Nitric Oxide during Experimental Murine Paracoccidioidomycosis

<b>Influence of n-3 Polyunsaturated Fatty Acid in the Proliferative Activity of Lymphocytes During Experimental Infection with <i>Paracoccidioides brasiliensis

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Production of TNF-α, nitric oxide and hydrogen peroxide by macrophages from mice with paracoccidioidomycosis that were fed a linseed oil-enriched diet

Cited by 9 publications

References 26 publications

Tumor necrosis factor-alpha 308G>A polymorphism and risk of rheumatic heart disease: a meta-analysis

Tumor necrosis factor-alpha 308G>A polymorphism and risk of rheumatic heart disease: a meta-analysis

Role of Omega-3 Polyunsaturated Fatty Acids in the Production of Prostaglandin E2and Nitric Oxide during Experimental Murine Paracoccidioidomycosis

<b>Influence of n-3 Polyunsaturated Fatty Acid in the Proliferative Activity of Lymphocytes During Experimental Infection with <i>Paracoccidioides brasiliensis

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Role of Omega-3 Polyunsaturated Fatty Acids in the Production of Prostaglandin E₂and Nitric Oxide during Experimental Murine Paracoccidioidomycosis