Antibodies against the Plasmodium falciparum glutamate-rich protein from naturally exposed individuals living in a Brazilian malaria-endemic area can inhibit in vitro parasite growth

Pratt-Riccio, Lilian Rose; Bianco‐Júnior, Cesare; Totino, Paulo Renato Rivas; Perce-da-Silva, Daiana de Souza; Silva, Luciene Aquino; Riccio, Evelyn Kety Pratt; Ennes-Vidal, Vítor; Neves-Ferreira, Ana Gisele Costa; Perales, Jonás; Rocha, Surza Lucia Gonçalves da; Dias-Da-Silva, Fabrício; Ferreira-da-Cruz, Maria de Fátima; Daniel-Ribeiro, Cláudio Tadeu; Oliveira-Ferreira, Joseli; Theisen, Michael; Carvalho, Lidiane dos Santos; Banic, Dalma Maria

doi:10.1590/s0074-02762011000900005

Cited by 14 publications

(12 citation statements)

References 62 publications

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“…Among the various polymorphic antigens, pfglurp also plays an important role by inducing protective immunity against malarial infection . In previous studies, it has been found that individuals who are naturally exposed to malaria in endemic regions have high levels of antibodies in response to glurp found to inhibit the in‐vitro growth of P. falciparum , which may happen with or without the help of monocytes, suggesting the possible role of glurp in controlling parasitaemia . Over time, there is a trend of diagnosing a large number of alleles, which is in direct relationship between the endemicity and number of samples evaluated in case of glurp .…”

Section: Discussionmentioning

confidence: 99%

Genetic diversity of Plasmodium falciparum merozoite surface protein‐1 (block 2), glutamate‐rich protein and sexual stage antigen Pfs25 from Chandigarh, North India

Kaur

Sehgal

Goyal

et al. 2017

Tropical Med Int Health

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Section: Discussionmentioning

confidence: 99%

Genetic diversity of Plasmodium falciparum merozoite surface protein‐1 (block 2), glutamate‐rich protein and sexual stage antigen Pfs25 from Chandigarh, North India

Kaur

Sehgal

Goyal

et al. 2017

Tropical Med Int Health

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“…Similar levels of sensitivity were observed in mice infected with P. chabaudi adami DS or P. berghei ANKA. Although mitochondrial membrane potential dyes have previously been employed to assess parasite viability [34,42] and to determine parasitaemia [43,44] to the best of this author’s knowledge the combination of these with DNA specific dyes has not been used to quantify parasitaemia in vivo . In addition to JC-1 and Hoechst, selective, fluorescently labelled antibodies were employed to detect and exclude RBC progenitors and leukocytes, further improving the sensitivity of the assay.…”

Section: Discussionmentioning

confidence: 99%

A flow cytometric assay to quantify invasion of red blood cells by rodent Plasmodium parasites in vivo

Lelliott

Lampkin

McMorran

et al. 2014

Malar J

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BackgroundMalaria treatments are becoming less effective due to the rapid spread of drug resistant parasites. Increased understanding of the host/parasite interaction is crucial in order to develop treatments that will be less prone to resistance. Parasite invasion of the red blood cell (RBC) is a critical aspect of the parasite life cycle and is, therefore, a promising target for the development of malaria treatments. Assays for analysing parasite invasion in vitro have been developed, but no equivalent assays exist for in vivo studies. This article describes a novel flow cytometric in vivo parasite invasion assay.MethodsExperiments were conducted with mice infected with erythrocytic stages of Plasmodium chabaudi adami strain DS. Exogenously labelled blood cells were transfused into infected mice at schizogony, and collected blood samples stained and analysed using flow cytometry to specifically detect and measure proportions of labelled RBC containing newly invaded parasites. A combination of antibodies (CD45 and CD71) and fluorescent dyes, Hoechst (DNA) and JC-1 (mitochondrial membrane potential), were used to differentiate parasitized RBCs from uninfected cells, RBCs containing Howell-Jolly bodies, leukocytes and RBC progenitors. Blood cells were treated ex vivo with proteases to examine the effects on in vivo parasite invasion.ResultsThe staining and flow cytometry analysis method was accurate in determining the parasitaemia down to 0.013% with the limit of detection at 0.007%. Transfused labelled blood supported normal rates of parasite invasion. Protease-treated red cells resulted in 35% decrease in the rate of parasite invasion within 30 minutes of introduction into the bloodstream of infected mice.ConclusionsThe invasion assay presented here is a versatile method for the study of in vivo red cell invasion efficiency of Plasmodium parasites in mice, and allows direct comparison of invasion in red cells derived from two different populations. The method also serves as an accurate alternative method of estimating blood parasitaemia.

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“…Additionally, a recent study of controlled experimental P. falciparum infections demonstrated that non-immune individuals acquire GLURP antibodies following a single, brief low-density P. falciparum infection (Turner et al 2011). Moreover, it was recently shown that the antibodies produced in response to GLURP in naturally exposed individuals can inhibit the in vitro growth of P. falciparum with or without cooperation from monocytes, suggesting that GLURP could play an important role in controlling parasitaemia (Pratt-Riccio et al 2011). …”

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confidence: 99%

Genetic polymorphisms in the glutamate-rich protein of Plasmodium falciparum field isolates from a malaria-endemic area of Brazil

Pratt-Riccio¹,

Perce-da-Silva

Lima-Junior

et al. 2013

Mem. Inst. Oswaldo Cruz

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The genetic diversity displayed by Plasmodium falciparum, the most deadly Plasmodium species, is a significant obstacle for effective malaria vaccine development. In this study, we identified genetic polymorphisms in P. falciparum glutamate-rich protein (GLURP), which is currently being tested in clinical trials as a malaria vaccine candidate, from isolates found circulating in the Brazilian Amazon at variable transmission levels. The study was performed using samples collected in 1993 and 2008 from rural villages situated near Porto Velho, in the state of Rondônia. DNA was extracted from 126 P. falciparum-positive thick blood smears using the phenol-chloroform method and subjected to a nested polymerase chain reaction protocol with specific primers against two immunodominant regions of GLURP, R0 and R2. Only one R0 fragment and four variants of the R2 fragment were detected. No differences were observed between the two time points with regard to the frequencies of the fragment variants. Mixed infections were uncommon. Our results demonstrate conservation of GLURP-R0 and limited polymorphic variation of GLURP-R2 in P. falciparum isolates from individuals living in Porto Velho. This is an important finding, as genetic polymorphisms in B and T-cell epitopes could have implications for the immunological properties of the antigen.

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Antibodies against the Plasmodium falciparum glutamate-rich protein from naturally exposed individuals living in a Brazilian malaria-endemic area can inhibit in vitro parasite growth

Cited by 14 publications

References 62 publications

Genetic diversity of Plasmodium falciparum merozoite surface protein‐1 (block 2), glutamate‐rich protein and sexual stage antigen Pfs25 from Chandigarh, North India

Genetic diversity of Plasmodium falciparum merozoite surface protein‐1 (block 2), glutamate‐rich protein and sexual stage antigen Pfs25 from Chandigarh, North India

A flow cytometric assay to quantify invasion of red blood cells by rodent Plasmodium parasites in vivo

Genetic polymorphisms in the glutamate-rich protein of Plasmodium falciparum field isolates from a malaria-endemic area of Brazil

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