Genetic diversity of <i>Plasmodium falciparum</i> merozoite surface protein‐1 (block 2), glutamate‐rich protein and sexual stage antigen Pfs25 from Chandigarh, North India

Kaur, Harpreet; Sehgal, Rakesh; Goyal, Kapil; Makkar, Nikita; Yadav, Richa; Bharti, Praveen K.; Singh, Niraj Kumar; Sarmah, Nilanju P; Mohapatra, Pradyumna K.; Mahanta, Jagadish; Bansal, Devendra; Sultan, Ali A.; Kanwar, Jagat R.

doi:10.1111/tmi.12990

Cited by 17 publications

(17 citation statements)

References 41 publications

(44 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In the present study, Pfmsp-1 locus exhibited 13 different genotypes and K1 allelic family was the most frequently observed followed by MAD20 and RO33. This finding is in contrast with previous studies from neighbouring Arunachal Pradesh and Chandigarh, North India where RO33 allelic frequency was highest followed by MAD20 and K1 33,34 . However, MAD20 was found to be more frequent than K1 and RO33 in Central India and Bangladesh 35,36 .…”

Section: Discussioncontrasting

confidence: 99%

Characterization of drug resistance and genetic diversity of Plasmodium falciparum parasites from Tripura, Northeast India

Patgiri

Sarma

Sarmah

et al. 2019

Sci Rep

View full text Add to dashboard Cite

Monitoring of anti-malarial drug resistance is vital in Northeast India as this region shares its international border with Southeast Asia. Genetic diversity of Plasmodium parasites regulates transmission dynamics, disease severity and vaccine efficacy. P. falciparum chloroquine resistance transporter (Pfcrt), multidrug resistance-1 (Pfmdr-1) and kelch 13 propeller (PfK-13) genes which govern antimalarial drug resistance and three genetic diversity markers, merozoite surface protein 1 and 2 (Pfmsp-1, Pfmsp-2) and glutamate rich protein (Pfglurp) were evaluated from Tripura, Northeast India using molecular tools. In the Pfcrt gene, 87% isolates showed triple mutations at codons M74I, N75E and K76T. 12.5% isolates in Pfmdr-1 gene showed mutation at N86Y. No polymorphism in PfK-13 propeller was found. Polyclonal infections were observed in 53.85% isolates and more commonly in adults (p = 0.0494). In the Pfmsp-1 locus, the K1 allelic family was predominant (71.2%) followed by the 3D7/IC family (69.2%) in the Pfmsp-2 locus. RII region of Pfglurp exhibited nine alleles with expected heterozygosity of 0.85. The multiplicity of infection for Pfmsp-1, Pfmsp-2 and Pfglurp were 1.56, 1.31 and 1.06 respectively. Overall, the study demonstrated a high level of chloroquine resistance and extensive parasite diversity in the region, necessitating regular surveillance in this population group.

show abstract

Section: Discussioncontrasting

confidence: 99%

Characterization of drug resistance and genetic diversity of Plasmodium falciparum parasites from Tripura, Northeast India

Patgiri

Sarma

Sarmah

et al. 2019

Sci Rep

View full text Add to dashboard Cite

show abstract

“…The nested PCR primers (NF and NR) for Pvcsp and Pvs25 was then utilized to perform Sanger sequencing (Genewiz INC, NJ, USA) in forward and reverse directions for all the purified products. The obtained sequences were then edited and analysed to see the intraspecific variation (SNPs) if any, among the sequences [10]. To investigate the genetic diversity among the Pvcsp and Pvs25 genes, MEGA vs 7.0.21 software was used [19].…”

Section: Methodsmentioning

confidence: 99%

“…Knowledge of the extent of genetic diversity enables the prediction of a pattern of emergence and spread of phenotypes of novel antigenic variants which leads to drug resistance or escape of vaccine-induced immunity, which might be responsible for the development of severe vivax malaria [8, 9]. Over the past few years extensive studies have been undertaken to understand the genetic diversity of P. falciparum , however there is a scarcity of literature on P. vivax genetic diversity [10].…”

Section: Introductionmentioning

confidence: 99%

Exploration of genetic diversity of Plasmodium vivax circumsporozoite protein (Pvcsp) and Plasmodium vivax sexual stage antigen (Pvs25) among North Indian isolates

Kaur

Sehgal

Kumar

et al. 2019

Malar J

Self Cite

View full text Add to dashboard Cite

Background Malaria is one of the important vector-borne diseases with high fatality rates in tropical countries. The pattern of emergence and spread of novel antigenic variants, leading to escape of vaccine-induced immunity might be factors responsible for severe malaria. A high level of polymorphism has been reported among malarial antigens which are under selection pressure imposed by host immunity. There are limited reports available on comparative stage-specific genetic diversity among Plasmodium vivax candidate genes in complicated vivax malaria. The present study was planned to study genetic diversity ( Pvcsp and Pvs25 ) among complicated and uncomplicated P. vivax isolates. Methods Pvcsp and Pvs2 -specific PCRs and DNA sequencing were performed on P. vivax PCR positive samples. Genetic diversity was analysed using appropriate software. Results The present study was carried out on 143 P. vivax clinical isolates, collected from Postgraduate Institute of Medical Education and Research, Chandigarh. Among the classic and variant types of Pvcsp , the VK210 (99%; 115/116) was found to be predominant in both complicated and uncomplicated group isolates. Out of the various peptide repeat motifs (PRMs) observed, GDRADGQPA (PRM1) and GDRAAGQPA (PRM2) was the most widely distributed among the P. vivax isolates. Whereas among the Pvs25 isolates, 100% of double mutants (E97 Q /I130 T ) in both the complicated (45/45) as well as in the uncomplicated (81/81) group was observed. Conclusion An analysis of genetic variability enables an understanding of the role of genetic variants in severe vivax malaria.

show abstract

“…PfGLURP or PfMSP-3 are expressed by all developmental stages of P. falciparum (pre-erythrocytic and erythrocytic stages) and on the surface of P. falciparum merozoites, respectively (Jordan et al, 2009; Kaur et al, 2017). These two P. falciparum -derived proteins are considered highly immunogenic and have been considered as vaccine candidates alone or in combination as fused proteins (e.g., GMZ2, a fusion of PfGLURP and PfMSP3) in clinical settings (Oeuvray et al, 1994a,b; Roussilhon et al, 2007; Belard et al, 2011).…”

Section: Protozoan Parasitesmentioning

confidence: 99%

Interleukin (IL)-21 in Inflammation and Immunity During Parasitic Diseases

Solaymani-Mohammadi

Eckmann

Singer

2019

Front. Cell. Infect. Microbiol.

View full text Add to dashboard Cite

Parasitic diseases cause significant morbidity and mortality in the developing and underdeveloped countries. No efficacious vaccines are available against most parasitic diseases and there is a critical need for developing novel vaccine strategies for care. IL-21 is a pleiotropic cytokine whose functions in protection and immunopathology during parasitic diseases have been explored in limited ways. IL-21 and its cognate receptor, IL-21R, are highly expressed in parasitized organs of infected humans as well in murine models of the human parasitic diseases. Prior studies have indicated the ability of the IL-21/IL-21R signaling axis to regulate the effector functions (e.g., cytokine production) of T cell subsets by enhancing the expression of T-bet and STAT4 in human T cells, resulting in an augmented production of IFN-γ. Mice deficient for either IL-21 (Il21−/−) or IL-21R (Il21r−/−) showed significantly reduced inflammatory responses following parasitic infections as compared with their WT counterparts. Targeting the IL-21/IL-21R signaling axis may provide a novel approach for the development of new therapeutic agents for the prevention of parasite-induced immunopathology and tissue destruction.

show abstract

Genetic diversity of Plasmodium falciparum merozoite surface protein‐1 (block 2), glutamate‐rich protein and sexual stage antigen Pfs25 from Chandigarh, North India

Abstract: Our findings document the genetic diversity among circulating strains of P. falciparum in an area of India with low malaria transmission and could have implications for control strategies to reach the national goal of malaria elimination.

Cited by 17 publications

References 41 publications

Characterization of drug resistance and genetic diversity of Plasmodium falciparum parasites from Tripura, Northeast India

Characterization of drug resistance and genetic diversity of Plasmodium falciparum parasites from Tripura, Northeast India

Exploration of genetic diversity of Plasmodium vivax circumsporozoite protein (Pvcsp) and Plasmodium vivax sexual stage antigen (Pvs25) among North Indian isolates

Interleukin (IL)-21 in Inflammation and Immunity During Parasitic Diseases

Contact Info

Product

Resources

About