Occurrence of blaOXA-23 gene in imipenem-susceptible Acinetobacter baumannii

Carvalho, Karyne Rangel; Carvalho-Assef, Ana Paula D’Alincourt; Santos, Lia Galvão dos; Pereira, Maria Jose; Asensi, Marise Dutra

doi:10.1590/s0074-02762011000400020

Cited by 22 publications

(19 citation statements)

References 11 publications

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“…1). This result is in accordant with the results being reported in Carvalho et al (2011). Reports hypothesized that the versatility of plasmids together with the abuse of antimicrobials in human medicine may have largely contributed to the spread of antimicrobial resistance worldwide (Carattoli, 2009).…”

Section: Discussionsupporting

confidence: 90%

First Report of the blaOXA-23 Gene in a Clinical Isolates of Acinetobacter baumannii in Najaf Hospitals-Iraq

Alsehlawi¹,

Alshara²,

Hadi³

et al. 2015

Research J. of Microbiology

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Carbapenemase producing Acinetobacter baumannii is frequently associated with nosocomial infections. Increasing resistance to carbapenems, may significantly reduce the choice of effective antibiotics. This study was conducted to determine the occurrence of carbapenemase producing A. baumannii isolates obtained from Najaf hospitals. Isolates were identified according to API 20NE system and more confirmed using 16Sr RNA gene. Carbapenem susceptibility was assayed by microbroth dilution and other antibiotics using disks diffusion test. Phenotypic detection of carbapenemase was performed using the imipenem-EDTA disk and modified Hodg tests. Then isolates were subjected to monoplex PCR targeting blaOXA-23, blaIMP and blaVIM genes. Twelve (1.5.%) A. baumannii isolates were recovered from clinical infections. Five (41.6%) of isolates were found to be imipenem and meropenem resistant (MIC ranged 128-256 μg mLG 1 ), of which, 4 (66.6%) gave positive result with the imipenem-EDTA disk and modified Hodg test. PCR experiments showed only two (33.3%) isolates were harbored blaOXA-23 gene. The present findings suggest that emergence of OXA-23 carbapenemase-producing A. baumannii clinical isolates in Najaf hospitals.

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Section: Discussionsupporting

confidence: 90%

First Report of the blaOXA-23 Gene in a Clinical Isolates of Acinetobacter baumannii in Najaf Hospitals-Iraq

Alsehlawi¹,

Alshara²,

Hadi³

et al. 2015

Research J. of Microbiology

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“…Exceptions included: strain 080502/0184 positive for the bla OXA-23 -like CHDL gene but sensitive to carbapenem and two strains (NCTC 13423 and LUH 5875 (European epidemic clone III)) negative for the bla OXA-23 -like CHDL gene but resistant to carbapenem. Carbapenem-susceptibility of the strain 080502/0184 can be explained by the absence of insertion sequence IS Aba1 in upstream position of bla OXA-23 -like CHDL gene as mentioned above [58], [59]. In case of the strains NCTC 13423 and LUH 5875 (European epidemic clone III) it is known that carbapenem resistance is expressed due to the insertion sequence IS Aba1 linked to bla OXA-51 -like CHDL gene in upstream position [28], [60].…”

Section: Resultsmentioning

confidence: 99%

Characterization of Newly Isolated Lytic Bacteriophages Active against Acinetobacter baumannii

et al. 2014

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Based on genotyping and host range, two newly isolated lytic bacteriophages, myovirus vB_AbaM_Acibel004 and podovirus vB_AbaP_Acibel007, active against Acinetobacter baumannii clinical strains, were selected from a new phage library for further characterization. The complete genomes of the two phages were analyzed. Both phages are characterized by broad host range and essential features of potential therapeutic phages, such as short latent period (27 and 21 min, respectively), high burst size (125 and 145, respectively), stability of activity in liquid culture and low frequency of occurrence of phage-resistant mutant bacterial cells. Genomic analysis showed that while Acibel004 represents a novel bacteriophage with resemblance to some unclassified Pseudomonas aeruginosa phages, Acibel007 belongs to the well-characterized genus of the Phikmvlikevirus. The newly isolated phages can serve as potential candidates for phage cocktails to control A. baumannii infections.

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“…The only isolate wherein ISAba1 was not located upstream of the bla OXA-23-like gene showed susceptibility to carbapenems. Thus, the presence of bla OXA-23-like without the ISAba1 promoter sequence might not be suffi cient to cause carbapenem hydrolysis, as reported by Carvalho et al (40) OXA-51 carbapenemases encoded by intrinsic (chromosomal) genes have a weak capacity to hydrolyze carbapenems (11) (41) . The presence of ISAba1 may provide a strong promoter for the bla OXA-51-like gene, thereby increasing its expression and causing resistance (42) (43) (44) .…”

Section: Discussionmentioning

confidence: 96%

The role of the genetic elements bla oxa and IS Aba 1 in the Acinetobacter calcoaceticus-Acinetobacter baumannii complex in carbapenem resistance in the hospital setting

Kobs¹,

Ferreira

Bobrowicz

et al. 2016

Rev. Soc. Bras. Med. Trop.

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Introduction: Members of the Acinetobacter genus are key pathogens that cause healthcare-associated infections, and they tend to spread and develop new antibiotic resistance mechanisms. Oxacillinases are primarily responsible for resistance to carbapenem antibiotics. Higher rates of carbapenem hydrolysis might be ascribed to insertion sequences, such as the ISAba1 sequence, near bla OXA genes. The present study examined the occurrence of the genetic elements bla OXA and ISAba1 and their relationship with susceptibility to carbapenems in clinical isolates of the Acinetobacter calcoaceticus-Acinetobacter baumannii complex. Methods: Isolates identifi ed over 6 consecutive years in a general hospital in Joinville, Southern Brazil, were evaluated. The investigation of 5 families of genes encoding oxacillinases and the ISAba1 sequence location relative to bla OXA genes was conducted using polymerase chain reaction. Results: All isolates presented the bla OXA-51-like gene (n = 78), and 91% tested positive for the bla OXA-23-like gene (n = 71). The presence of ISAba1 was exclusively detected in isolates carrying the bla OXA-23-like gene. All isolates in which ISAba1 was found upstream of the bla OXA-23-like gene (n = 69) showed resistance to carbapenems, whereas the only isolate in which ISAba1 was not located near the bla OXA-23-like gene was susceptible to carbapenems. The ISAba1 sequence position of another bla OXA-23-like -positive isolate was inconclusive. The isolates exclusively carrying the bla OXA-51-like gene (n = 7) showed susceptibility to carbapenems. Conclusions: The presence of the ISAba1 sequence upstream of the bla OXA-23-like gene was strongly associated with carbapenem resistance in isolates of the A. calcoaceticus-A. baumannii complex in the hospital center studied.

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Occurrence of blaOXA-23 gene in imipenem-susceptible Acinetobacter baumannii

Cited by 22 publications

References 11 publications

First Report of the blaOXA-23 Gene in a Clinical Isolates of Acinetobacter baumannii in Najaf Hospitals-Iraq

First Report of the blaOXA-23 Gene in a Clinical Isolates of Acinetobacter baumannii in Najaf Hospitals-Iraq

Characterization of Newly Isolated Lytic Bacteriophages Active against Acinetobacter baumannii

The role of the genetic elements bla oxa and IS Aba 1 in the Acinetobacter calcoaceticus-Acinetobacter baumannii complex in carbapenem resistance in the hospital setting

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