High vancomycin resistance among biofilms produced by Staphylococcus species isolated from central venous catheters

Antunes, Ana Lúcia Souza; Bonfanti, Jéssica Weis; Perez, Leandro Reus Rodrigues; Pinto, Camille Cattani Ferreira; Freitas, Aline Kellermann de; Macêdo, Alexandre José; Barth, Afonso Luís

doi:10.1590/s0074-02762011000100008

Cited by 34 publications

(20 citation statements)

References 22 publications

(24 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Likewise, MIC ADH and MBEC values were widely distant from each other; biofilm-eradicating concentrations varied from 8-to 64-fold higher than biofilm-inhibiting concentrations. Vancomycin susceptibility against biofilm-forming staphylococci was previously studied in Brazil 9 and showed alarming results-as also demonstrated in this study-because this drug is the primary choice for antimicrobial and empirical treatment.…”

Section: Discussionsupporting

confidence: 51%

“…This antimicrobial antibiofilm activity already was evaluated and seemed to be highly powerless regarding complete biofilm eradication requirement 8,9 . Rifampicin has putative antibiofilm properties, ability to penetrate staphylococcal biofilm 10 , and had demonstrated promising utility as agent for eradicating S. aureus biofilm alone 8 or in combination with other drugs especially for device-related infections [11][12][13][14] .…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Rifampicin fails to eradicate mature biofilm formed by methicillin-resistant Staphylococcus aureus

Reiter

Sambrano

Villa

et al. 2012

Rev. Soc. Bras. Med. Trop.

View full text Add to dashboard Cite

Introduction: Antimicrobial activity on biofilms depends on their molecular size, positive charges, permeability coefficient, and bactericidal activity. Vancomycin is the primary choice for methicillin-resistant Staphylococcus aureus (MRSA) infection treatment; rifampicin has interesting antibiofilm properties, but its effectivity remains poorly defined. Methods: Rifampicin activity alone and in combination with vancomycin against biofilm-forming MRSA was investigated, using a twofold serial broth microtiter method, biofilm challenge, and bacterial count recovery. Results: Minimal inhibitory concentration (MIC) and minimal bactericidal concentration for vancomycin and rifampicin ranged from 0.5 to 1mg/l and 0.008 to 4mg/l, and from 1 to 4mg/l and 0.06 to 32mg/l, respectively. Mature biofilms were submitted to rifampicin and vancomycin exposure, and minimum biofilm eradication concentration ranged from 64 to 32,000 folds and from 32 to 512 folds higher than those for planktonic cells, respectively. Vancomycin (15mg/l) in combination with rifampicin at 6 dilutions higher each isolate MIC did not reach in vitro biofilm eradication but showed biofilm inhibitory capacity (1.43 and 0.56log 10 CFU/ml reduction for weak and strong biofilm producers, respectively; p<0.05). Conclusions: In our setting, rifampicin alone failed to effectively kill biofilm-forming MRSA, demonstrating stronger inability to eradicate mature biofilm compared with vancomycin.

show abstract

Section: Discussionsupporting

confidence: 51%

Section: Introductionmentioning

confidence: 99%

Rifampicin fails to eradicate mature biofilm formed by methicillin-resistant Staphylococcus aureus

Reiter

Sambrano

Villa

et al. 2012

Rev. Soc. Bras. Med. Trop.

View full text Add to dashboard Cite

show abstract

“…However, recent reports of linezolid resistance among coagulase-negative Staphylococcus (CNS) at medical centres raises concerns (Potoski et al, 2006;Dandache et al, 2009). Because of the high prevalence of CNS biofilm in our setting (Antunes et al, 2010(Antunes et al, , 2011Reiter et al, 2011) and around the world (Dandache et al, 2009;Fredheim et al, 2009;Jain & Agarwal, 2009), and the increasing prevalence of higher antimicrobial resistance rates among these biofilm-forming isolates (Cha et al, 2011), future potential treatments for staphylococcal infections mediated by the formation of biofilms are compromised.…”

Section: Resultsmentioning

confidence: 99%

“…Molecular mechanisms of antimicrobial resistance in biofilm are not the same as for planktonic bacteria, since biofilm formation is accompanied by global genetic regulatory changes. The biofilm lifestyle affords bacteria a 10-to 1000-fold increase in antimicrobial resistance compared to their planktonic counterparts, and many of these genetic changes render the constituent bacteria resistant to antimicrobials (Mah & O'Toole, 2001;Stewart & Costerton, 2001;Patel, 2005;Antunes et al, 2011). The resistance of biofilm to antimicrobials may be associated with limited antimicrobial diffusion through the biofilm matrix (Suci et al, 1994), physiological changes (Dagostino et al, 1991) and a reduced growth rate of bacteria in biofilms (Duguid et al, 1992).…”

Section: Introductionmentioning

confidence: 99%

Inhibition of biofilm maturation by linezolid in meticillin-resistant Staphylococcus epidermidis clinical isolates: comparison with other drugs

Reiter

Villa

Paim

et al. 2013

Journal of Medical Microbiology

View full text Add to dashboard Cite

Biofilm resistance mechanisms are multifactorial and vary from one organism to another. The purpose of this study was to investigate the efficacy of linezolid against indwelling device-related meticillin-resistant Staphylococcus epidermidis (MRSE) biofilm, and compare this with other antimicrobials. MICs, minimum biofilm inhibitory concentrations (MBICs) and minimum biofilm eradication concentrations (MBECs) were determined by the microtitre plate method. Fourteen and thirteen isolates from patients with indwelling device-related bacteraemia (IDB) and indwelling device colonization not associated with bacteraemia, respectively, were assessed. High MBIC was associated with a high intensity of biofilm formation (gentamicin r50.796; linezolid r50.477; rifampicin r50.634; tigecycline r50.410; and vancomycin r50.771), but this correlation was not observed with MBEC. Linezolid demonstrated better in vitro antimicrobial activity than other antimicrobials (MBIC -gentamicin P,0.001, rifampicin P50.019, vancomycin P50.008; MBEC -gentamicin P,0.001, rifampicin P50.002, vancomycin P,0.001). Biofilm growth inhibition was strongly associated with biofilm formation intensity; however, biofilm eradication was not cell number dependent. MRSE biofilm eradication would represent a huge advance for IDB, although high concentrations of gentamicin, linezolid, rifampicin, tigecycline and vancomycin were required for that. In general, linezolid reached better in vitro concentrations and was demonstrated to be highly active against MRSE biofilms by inhibiting their growth during biofilm formation.

show abstract

“…Glycopeptide antibiotics, like vancomycin, may fail to treat these infections due to a diminished antimicrobial effect on bacteria in the biofilm environment [11]. Therefore, the minimum biofilm eradication concentration is more important than the minimal inhibitory concentration in this setting [12]. Topical vancomycin may have been ineffective in this suture-related case due to the production of a biofilm by S. epidermidis.…”

Section: Discussionmentioning

confidence: 99%

A case of suture-related bacterial keratitis and its treatment with topical imipenem

Türkyılmaz

Kurt

Dilek

et al. 2011

j ocul biol dis inform

View full text Add to dashboard Cite

High vancomycin resistance among biofilms produced by Staphylococcus species isolated from central venous catheters

Cited by 34 publications

References 22 publications

Rifampicin fails to eradicate mature biofilm formed by methicillin-resistant Staphylococcus aureus

Rifampicin fails to eradicate mature biofilm formed by methicillin-resistant Staphylococcus aureus

Inhibition of biofilm maturation by linezolid in meticillin-resistant Staphylococcus epidermidis clinical isolates: comparison with other drugs

A case of suture-related bacterial keratitis and its treatment with topical imipenem

Contact Info

Product

Resources

About