IgG and IgM western blot assay for diagnosis of congenital toxoplasmosis

Machado, Anderson Silva; Andrade, Gláucia Manzan Queiroz de; Januário, José Nélio; Fernandes, Matheus Delgado; Carneiro, Ana Carolina Aguiar Vasconcelos; Carneiro, Mariângela; Carellos, Ericka Viana Machado; Romanelli, Roberta Maia de Castro; Vasconcelos-Santos, Daniel Vítor; Vítor, Ricardo Wagner Almeida

doi:10.1590/s0074-02762010000600005

Cited by 23 publications

(16 citation statements)

References 15 publications

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“…Table 1 provides an overview of all studies included in the final sample and of all data elements used during the data analysis process. The 41 studies were distributed into the previously determined three categories as follows: DIAGNOSTIC METHODS [2,4,5,8,9,12,14,16,17,20,25,27,29,32,34,35,37,41,42 (nineteen studies)]; DIAGNOSIS IN PREGNANT WOMEN [ 3,7,10,14,25,27,35,42,46,50,51,53, 59 thirteen studies)]; DIAGNOSIS IN NEONATES [7,8,11,20,61,64,65,67,68,70,71,72 (twelve studies)] and DIAGNOSIS IN INFANTS [1,3,5,9,11,12,20,27,…”

Section: Resultsmentioning

confidence: 99%

Diagnosing congenital toxoplasmosis: where are we? A systematic review

Santos¹,

Silva²,

Oliveira³

et al. 2016

Int Arch Med

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Purpose: Compile information on laboratory methods for diagnosis of congenital toxoplasmosis, considering the tests conducted since the gestational stage until the child period. Methods:A systematic review of 01.01.2006 to 31.12.2013 was held by VHL (Virtual Health Library). The search was performed with the descriptors ''toxoplasmosis" and "diagnosis. The selected articles were indexed in MEDLINE. The information pertinent to the study was selected, categorized and analyzed. Of the 186 articles found, 41 met the eligibility criteria.

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Section: Resultsmentioning

confidence: 99%

Diagnosing congenital toxoplasmosis: where are we? A systematic review

Santos¹,

Silva²,

Oliveira³

et al. 2016

Int Arch Med

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“…101 The true incidence of CT in the United States might be even higher, because the sensitivity of the newborn screening test (filter paper blot spot Toxoplasma-IgM) used in the New England Neonatal Screening Program is approximately 50% to 75%. 77,102,105 The incidence of CT may also be higher in areas of the United States where the seroprevalence of T gondii infections is higher. Moreover, neonatal screening programs might also underestimate the true incidence of CT, because fetal losses attributable to severe CT were not counted.…”

Section: United Statesmentioning

confidence: 99%

Diagnosis, Treatment, and Prevention of Congenital Toxoplasmosis in the United States

Maldonado¹,

Read²,

et al. 2017

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“…In fact, the immunoblot is a sensitive rapid assay that detects specific antibodies as described in other studies. [7][8][9] The positivity of western blot assay was observed in the first 2 months of life, which provides an early diagnosis in babies for whom antenatal diagnosis was negative or not carried out because the other serological tests (ELISA or ISAGA assays) could not detect IgM antibodies at birth. Nevertheless, as reported in other studies, 10,11 IgM detection remains useful mainly in newborns of women infected lately during their pregnancy as observed in cases no 15, 22, 24, 27, and 32.…”

Section: Discussionmentioning

confidence: 99%

Congenital Toxoplasmosis in Tunisia: Prenatal and Neonatal Diagnosis and Postnatal Follow-up of 35 Cases

Boudaouara

Karim

Maatoug

et al. 2018

The American Journal of Tropical Medicine and Hygiene

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Congenital toxoplasmosis (CT) results from transplacental passage of to the fetus during acute maternal infection. Our study aims to report clinical and biological patterns of 35 cases of CT diagnosed at the department of the Parasitology of the Pasteur Institute of Tunis and to access the performance of prenatal and early postnatal diagnosis techniques. Serological screening of maternal infection was performed by Immunoglobulin (Ig) M and IgG detection and IgG avidity determination. Prenatal diagnosis was based on both DNA detection in the amniotic fluid and monthly ultrasound examinations. polymerase chain reaction analysis on amniotic fluid, performed only in 15 cases, detected 's DNA in five cases (33.3%). Ultrasound examination did not reveal any morphological abnormalities. Thirty newborns had serological criteria of infection. Congenital toxoplasmosis diagnosis was confirmed in 23 cases (76.6%) by immunoblot. Among the 35 born-infants, five (14.3%) were symptomatic: three had chorioretinitis at the first clinical ocular examination, one had neurological symptoms (seizures) with positive parasite DNA in cerebral spinal fluid, and one had both ophthalmological and neurological damages- chorioretinitis and intracranial calcifications in the computed tomography scan. Thirty-four of 35 infected children were treated with pyrimethamine-sulfadiazine combination. Four (11.7%) of the treated infants showed abnormal hematological values because of the treatment side effect. Serological rebound was observed in seven infants. A screening program and a diagnostic algorithm in pregnant women should be implemented in Tunisia to improve the follow-up of seronegative ones and to prevent CT cases.

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IgG and IgM western blot assay for diagnosis of congenital toxoplasmosis

Cited by 23 publications

References 15 publications

Diagnosing congenital toxoplasmosis: where are we? A systematic review

Diagnosing congenital toxoplasmosis: where are we? A systematic review

Diagnosis, Treatment, and Prevention of Congenital Toxoplasmosis in the United States

Congenital Toxoplasmosis in Tunisia: Prenatal and Neonatal Diagnosis and Postnatal Follow-up of 35 Cases

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