Marine Pseudomonas putida: a potential source of antimicrobial substances against antibiotic-resistant bacteria

Marinho, Palloma Rodrigues; Moreira, Ana Paula B.; Pellegrino, Flávia Lúcia Piffano Costa; Muricy, Guilherme; Bastos, Maria do Carmo de Freire; Santos, Kátia Regina Netto dos; Giambiagi-deMarval, Márcia; Laport, Marinella Silva

doi:10.1590/s0074-02762009000500002

Cited by 36 publications

(25 citation statements)

References 17 publications

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“…The selected strain when cultured in Brain Heart Infusion Broth (BHIB), showed higher activity against tested microbial pathogens. Our observation of BHIB supporting the highest level of accumulation of crude secondary metabolites is in concert with the findings of Marinho et al (2009) who reported that BHI supported growth of a bacteriocin producer strain of P. putida. In this study, we used the zones of inhibition of the lawn of bacterial cells around extracts filled disc as an indication of antibacterial activity.…”

Section: Discussionsupporting

confidence: 91%

Anti-Microbial Properties of Secondary Metabolites of Marine Gordonia tearrae Extract

Elfalah¹,

Usup²,

Ali³

2013

JAS

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The objective of this study is to screen four species of marine actinomycetes: Gordonia (SPTG111), Rhodococcus sp (BP33), Gordonia sp (BP5), Brevibacterium antarcterium (SPTG45). These bacteria were grown in seven different media, namely Braine Heart Infusion broth (BHIB), Nutrient broth, Marine broth, Trypticasein Soy Broth, Tryptic Soy Broth, Luria Bertani broth, and Lauryl Sulfate Broth. Brine heart infusion broth (BHIB) was used to growth bacteria for the antibacterial activity test. Crude extract of Gordonia tearrae was tested for antimicrobial activity against Staphylococcus aureus, methicillin resistant Staphylococcus aureus (MRSA), Bacillus cereus, Enterococcus faecalis, Escherichia coli, Salmonella typhi, Pseudomonas aeruginosa and Candida albicans using agar diffusion method. The minimal inhibitory concentration (MIC) of G. tearrae crude extract was determined by the microbroth dilution method. Ethyl acetate extract of this isolate showed significant antibacterial activity against Gram negative bacteria E. coli (20 mm), Pseudomonas aeruginosa (9 mm), Salmonella typhi (12 mm) and Gram positive bacteria E. faecalis (7 mm), Bacillus cereus (21 mm), and MRSA (30 mm), and yeast C. albicans (21 mm). The MIC value of antimicrobial activity ranged from 25 to 6.3 µg/ml.

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Section: Discussionsupporting

confidence: 91%

Anti-Microbial Properties of Secondary Metabolites of Marine Gordonia tearrae Extract

Elfalah¹,

Usup²,

Ali³

2013

JAS

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“…Marine pseudomonads have been detected in bacterioplankton, in sediments (Engel et al 2002). However, very little is known about Pseudomonas strains isolated from marine sponges and their production of bioactive compounds (Santos et al 2010;Marinho et al 2009;Isnansetyo and Kamei 2009). However, very little is known about Pseudomonas strains isolated from marine sponges and their production of bioactive compounds (Santos et al 2010;Marinho et al 2009;Isnansetyo and Kamei 2009).…”

Section: Discussionmentioning

confidence: 99%

“…Mutants were screened on agar-BHI for their lack of antimicrobial activity against S. aureus ATCC 29213 as described previously (Marinho et al 2009). …”

Section: Transposon Mutagenesis Assaysmentioning

confidence: 99%

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Antibacterial activity and mutagenesis of sponge-associated Pseudomonas fluorescens H41

Santos-Gandelman

Hardoim

et al. 2015

Antonie van Leeuwenhoek

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Marine sponges (phylum Porifera) are well known to harbour a complex and diverse bacterial community. Some of these sponge-associated bacteria have been shown to be the real producers of secondary metabolites with a wide range of activities from antimicrobials to anticancer agents. Previously, we revealed that the strain Pseudomonas fluorescens H41 isolated from the sponge Haliclona sp. (collected at the coast of Rio de Janeiro, Brazil) showed a strong antimicrobial activity against clinical and marine bacteria. Thus, in this study the genes involved in the antimicrobial activity of P. fluorescens H41 were identified. To this end, a library of mutants was generated via miniTnphoA3 transposon mutagenesis and the resulting clones were characterized for their antimicrobial activity. It was demonstrated that genes involved in the biosynthesis of the pyoverdine siderophore are related to the inhibitory activity of P. fluorescens H41. Therefore, this strain might play an important role in the biocontrol of the host sponge.

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“…Indeed, the search of antimicrobial agents is an important strategy for the establishment of new alternative therapies in infections, which are difficult to handle [4]. …”

Section: Introductionmentioning

confidence: 99%

Phytochemical and Antibacterial Investigations of the Extracts and Fractions from the Stem Bark ofHymenaea stigonocarpaMart. ex Hayne and Effect on Ultrastructure ofStaphylococcus aureusInduced by Hydroalcoholic Extract

Dimech

Soares

Ferreira

et al. 2013

The Scientific World Journal

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The aim of this study was to investigate the antimicrobial activity of different extracts and fractions obtained from Hymenaea stigonocarpa stem barks. The cyclohexanic, ethyl acetate, ethanol, aqueous, and hydroalcoholic extracts were obtained by maceration. The hydroalcoholic extract was partitioned, which resulted in the ethyl acetate and aqueous fractions. All extracts and fractions were subjected to phytochemical screening and evaluation of total phenol and tannin contents. An HPLC-DAD and ultrastructural alterations analysis were performed. Terpenes and coumarins were detected in the cyclohexanic extract. Flavonoids and condensed tannins were present in the other extracts and fractions. The extracts with the highest contents of tannins, ethanol (EE), hydroalcoholic (HE), and aqueous fraction (AF) showed also the highest antimicrobial activity. The MIC values ranged from 64 to 526 µg/mL. The chromatographic fingerprints suggest the presence of astilbin and other flavonoids in EE and HE. Presence of the thick cell wall, undulating outer layer, abnormal septa, and leakage of the cytoplasmic contents and absence of cell wall and cell lyses were the main alterations observed on Staphylococcus aureus ATCC 33591 after treatment with the Hymenaea stigonocarpa hydroalcoholic extract. The presence of phenolic compounds like flavonoids and tannins is possibly the reason for the antimicrobial activity.

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Marine Pseudomonas putida: a potential source of antimicrobial substances against antibiotic-resistant bacteria

Cited by 36 publications

References 17 publications

Anti-Microbial Properties of Secondary Metabolites of Marine Gordonia tearrae Extract

Anti-Microbial Properties of Secondary Metabolites of Marine Gordonia tearrae Extract

Antibacterial activity and mutagenesis of sponge-associated Pseudomonas fluorescens H41

Phytochemical and Antibacterial Investigations of the Extracts and Fractions from the Stem Bark ofHymenaea stigonocarpaMart. ex Hayne and Effect on Ultrastructure ofStaphylococcus aureusInduced by Hydroalcoholic Extract

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