Evaluation of an enzyme-linked immunoelectrotransfer blot test for the confirmatory serodiagnosis of human toxocariasis

Roldán, William H.; Espinoza, Yrma

doi:10.1590/s0074-02762009000300003

Cited by 49 publications

(41 citation statements)

References 53 publications

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“…In the IgG-IB, sera from patients with toxocariasis recognized most of the protein bands, ranging from 29 to > 205 kDa. NUNES et al 17 found in the present study were not described by the other investigators 1,11,17,20 , and deserve to be further studied. Technical peculiarities used in TES production or the IB procedure may be the cause of such differences 1,17 .…”

Section: Discussioncontrasting

confidence: 57%

“…For diagnosis, IgG is the best marker as it was detected in all patients with visceral, ocular and mixed forms of the disease. Low MW bands, described by other researchers 11,20 as specific, may be targets for IgG antibody detection in the diagnosis of toxocariasis. For monitoring patients after chemotherapy, IgG to > 205 kDa fractions, IgA to 29-38, 48-54, 81-93 kDa and IgE to 95-121 kDa may be useful.…”

Section: Discussionmentioning

confidence: 93%

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Potential immunological markers for diagnosis and therapeutic assessment of toxocariasis

Rubinsky-Elefant

Hoshino‐Shimizu

Jacob

et al. 2011

Rev. Inst. Med. trop. S. Paulo

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SUMMARYIn human toxocariasis, there are few approaches using immunological markers for diagnosis and therapeutic assessment. An immunoblot (IB) assay using excretory-secretory Toxocara canis antigen was standardized for monitoring IgG, IgE and IgA antibodies in 27 children with toxocariasis (23 visceral, three mixed visceral and ocular, and one ocular form) for 22-116 months after chemotherapy. IB sensitivity was 100% for IgG antibodies to bands of molecular weight [29][30][31][32][33][34][35][36][37][38][48][49][50][51][52][53][54] >205 kDa,[48][49][50][51][52][53][54] > 205 kDa, and 65.4% for IgA to 29-38, 48-54, 81-93 kDa. Candidates for diagnostic markers should be IgG antibodies to bands of low molecular weight (29-38 and 48-54 kDa). One group of patients presented the same antibody reactivity to all bands throughout the follow-up study; in the other group, antibodies decayed partially or completely to some or all bands, but these changes were not correlated with time after chemotherapy. Candidates for monitoring patients after chemotherapy may be IgG antibodies to > 205 kDa fractions, IgA to 29-38, 48-54, 81-93 kDa and IgE to 95-121 kDa. Further identification of antigen epitopes related to these markers will allow the development of sensitive and specific immunoassays for the diagnosis and therapeutic assessment of toxocariasis.

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Section: Discussioncontrasting

confidence: 57%

Section: Discussionmentioning

confidence: 93%

Potential immunological markers for diagnosis and therapeutic assessment of toxocariasis

Rubinsky-Elefant

Hoshino‐Shimizu

Jacob

et al. 2011

Rev. Inst. Med. trop. S. Paulo

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“…Concentration of culture fluid containing ES antigen was achieved using PEG 20,000 in this study (Roldan and Espinoza 2009). Many authors reported the usage of ultra centrifugal devices with molecular weight cut off of 10,000 Da for concentration of culture fluid (Badley et al 1987;Bowman et al 1987 andKim et al 2008).…”

Section: Discussionmentioning

confidence: 86%

In vitro production of Toxocara canis excretory-secretory (TES) antigen

et al. 2014

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Toxocara canis is a widespread gastrointestinal nematode parasite of dogs and cause Toxocara larva migrans, an important zoonotic disease in humans on ingestion of infective eggs. Toxocarosis is one of the few human parasitic diseases whose serodiagnosis uses a standardized antigen, T. canis excretory secretory antigen (TES). The present study describes collection of T. canis adult worm, collection and embryonation of T. canis eggs, hatching and separation of T. canis larvae, in vitro maintenance of T. canis second stage larvae for production of TES, concentration of culture fluid TES and yield of TES in correlation with various methods cited in literature.

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“…Thus, it is not possible to evaluate the performance of a test by comparing it to another with unquestionable results. For this reason, the ELISA-IgG test was chosen as a reference in this work based on the high degree of correlation demonstrated with the Western blot method (López et al 2005a, Roldán & Espinoza 2009, which is considered a confirmatory test (Magnaval et al 2001); additionally, the ELISA-IgG test is the internationally accepted diagnostic test. The k concordance index has been described as a way to comparatively analyse the agreement between two diagnostic tests with qualitative results (positive or negative).…”

Section: Discussionmentioning

confidence: 99%

An evaluation of the dot-ELISA procedure as a diagnostic test in an area with a high prevalence of human Toxocara canis infection

Bojanich

Marino

López

et al. 2012

Mem. Inst. Oswaldo Cruz

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The aim of this work was to evaluate a dot-enzyme-linked immunosorbent assay (dot-ELISA) using excretory-secretory antigens from the larval stages of Toxocara canis for the diagnosis of toxocariasis. A secondary aim was to establish the optimal conditions for its use in an area with a high prevalence of human T. canis infection. The dot-ELISA test was standardised using different concentrations of the antigen fixed on nitrocellulose paper strips and increasing dilutions of the serum and conjugate. Both the dot-ELISA and standard ELISA methods were tested in parallel with the same batch of sera from controls and from individuals living in the problem area. The best results were obtained with 1.33 µg/mL of antigen, dilutions of 1/80 for the samples and controls and a dilution of 1/5,000 for the anti-human IgG-peroxidase conjugate. All steps of the procedure were performed at room temperature. The coincidence between ELISA and dot-ELISA was 85% and the kappa index was 0.72. The dot-ELISA test described here is rapid, easy to perform and does not require expensive equipment. Thus, this test is suitable for the serological diagnosis of human T. canis infection in field surveys and in the primary health care centres of endemic regions

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Evaluation of an enzyme-linked immunoelectrotransfer blot test for the confirmatory serodiagnosis of human toxocariasis

Cited by 49 publications

References 53 publications

Potential immunological markers for diagnosis and therapeutic assessment of toxocariasis

Potential immunological markers for diagnosis and therapeutic assessment of toxocariasis

In vitro production of Toxocara canis excretory-secretory (TES) antigen

An evaluation of the dot-ELISA procedure as a diagnostic test in an area with a high prevalence of human Toxocara canis infection

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