In vitro production of Toxocara canis excretory-secretory (TES) antigen

Thomas, Divyamol; Jeyathilakan, N.; Basith, S. Abdul; Senthilkumar, T.

doi:10.1007/s12639-014-0630-4

Cited by 11 publications

(5 citation statements)

References 40 publications

(69 reference statements)

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“…Production and purification of TES antigen was carried out according to Savigny et al , The resulting material was reconstituted in sterile Milli-Q water, and the quality of the prepared TES antigen was confirmed by SDS PAGE (Supporting Information, Figure S1), which showed the typical band profile of the TES antigen . The concentration of TES antigen in the stock solution was determined in triplicate by NanoDrop (OD280 nm of 1 corresponds to 1 mg/mL).…”

Section: Methodsmentioning

confidence: 99%

Fast One-Step Ultrasensitive Detection of Toxocara canis Antigens by a Nanobody-Based Electrochemical Magnetosensor

et al. 2019

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Human toxocariasis (HT) is a cosmopolitan zoonotic disease caused by the migration of the larval stage of the roundworm Toxocara canis. Current HT diagnostic methods do not discriminate between active and past infections. Here, we present a method to quantify Toxocara excretory/secretory antigen, aiming to identify active cases of HT. High specificity is achieved by employing nanobodies (Nbs), single domain antigen binding fragments from camelid heavy chain-only antibodies. High sensitivity is obtained by the design of an electrochemical magnetosensor with an amperometric read-out. Reliable detection of TES antigen at 10 and 30 pg/mL level was demonstrated in phosphate buffered saline and serum, respectively. Moreover, the assay showed no cross-reactivity with other nematode antigens. To our knowledge, this is the most sensitive method to quantify the TES antigen so far. It also has great potential to develop point of care diagnostic systems in other conditions where high sensitivity and specificity are required.

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Section: Methodsmentioning

confidence: 99%

Fast One-Step Ultrasensitive Detection of Toxocara canis Antigens by a Nanobody-Based Electrochemical Magnetosensor

et al. 2019

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“…On third day, the segregates were gathered, and exposure again to centrifugation (4000 rpm for 20minutes) at 4°C. Finally, the detaches were gathered and put away at (-80°) for serological measurement later [14].…”

Section: Hbvsvp Concentrationmentioning

confidence: 99%

Advanced Trends for Production of Hepatitis B Virus Subviral Particles Using Different Technique to Enhance Overcome of Hepatitis B Virus Infection

Selim

Mousa

Mansour

et al. 2020

Al-Azhar Bulletin of Science

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Hepatitis B infection (HBV) disease is one of the main hazard factors for chronic hepatitis, liver fibrosis, cirrhosis and hepatocellular malignant growth (HCC), which is a significant worldwide medical issue Hepatitis B virus (HBV) infection is one of the leading risk factors for chronic hepatitis, liver fibrosis, cirrhosis and hepatocellular cancer (HCC), which is a major global health problemeven with vaccine use and self-resolution in most cases. HBV contains different particle forms including non-infectious spherical and tubular sub viral particles (SVPs) with 22-nm-diameter that present potent immunogenicity. The main goal of the presented study was to optimize the best route for creation the SVP which discharged from incorporated HepG2.2.15 cell line in lab. For aim achievement, incorporated HepG2.2.15 cell line refined for SVP creation and fixation. SVP analyzed by using serological marker and electron microscopy. Outcomes demonstrated the development of HepG2.2.15 cell line in our complete media (Williams E media) with heat inactivated fetal cow-like serum (FBS), insulin and hydrocortisone bring about creation high measures of discharged viral particles in the supernatant. For recognizable proof of morphology and structures of SVP, electron microscopy indicated various shapes including circular and fibers for having a similar morphology of HBV virion, while it's completely different in the diameter. These findings shed light on an important technique used in production of huge number of SVP, which is important advance in Hepatitis BV irus irresistible rounds, and the gathering of HBV subviral particles might be basically connected to pathogenesis of the virus inorder to enhance overcome of HBV infection.

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“…The method of Thomas et al (2014) was used, with some modifications. Egg cultures were transferred to centrifuge tubes and were washed in distilled water by centrifugation for four times at 2000 rpm for 5 min to remove the formalin in the solution.…”

Section: Decorticationmentioning

confidence: 99%

Dot enzyme-linked immunosorbent assay (ELISA) for the detection of Toxocara infection using a rat model

Besana

Valdez

2017

J Parasit Dis

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Toxocariasis is a zoonotic disease usually caused by dog and cat roundworms, and Detection and diagnosis is difficult in paratenic and accidental hosts, including humans, as they cannot be detected through conventional methods such as fecal examination. Diagnosis therefore relies on immunological methods and molecular methods such as enzyme-linked immunosorbent assay (ELISA) and Western Blot, which are both time-consuming and requires sophisticated equipment. In the Philippines, only a few studies are available on seroprevalence. Therefore, there is a need to adapt methods for serodiagnosis of infection in humans for the Philippine setting. A dot enzyme linked immunosorbent assay (dot-ELISA) was standardized using excretory-secretory antigens. Test sera were collected from laboratory rats (Sprague-Dawley strain) experimentally infected with embryonated eggs of and as well as rice field rats naturally infected with and sp. Optimum conditions used were 20 µg/ml antigen concentration and 1:10 serum dilution. The sensitivity, specificity, positive, and negative predictive values were 90% (95% CI 55.5-99.7%), 100% (95% CI 69.2-100.0%), 100% (95% CI 66.4-100%), and 90.9% (95% CI 58.7-99.8%), respectively. Dot-ELISA has the potential to be developed as a cheaper, simpler, and more practical method for detection of anti- antibodies on accidental hosts. This is a preliminary study conducted on experimental animals before optimization and standardization for human serum samples.

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In vitro production of Toxocara canis excretory-secretory (TES) antigen

Cited by 11 publications

References 40 publications

Fast One-Step Ultrasensitive Detection of Toxocara canis Antigens by a Nanobody-Based Electrochemical Magnetosensor

Fast One-Step Ultrasensitive Detection of Toxocara canis Antigens by a Nanobody-Based Electrochemical Magnetosensor

Advanced Trends for Production of Hepatitis B Virus Subviral Particles Using Different Technique to Enhance Overcome of Hepatitis B Virus Infection

Dot enzyme-linked immunosorbent assay (ELISA) for the detection of Toxocara infection using a rat model

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