Polysaccharide-rich fraction of Agaricus brasiliensis enhances the candidacidal activity of murine macrophages

Martins, Priscila Raquel; Gameiro, Maria Carolina; Castoldi, Lindsey; Romagnoli, Graziela Gorete; Lopes, Fabiane Catanho; Pinto, Andréa Vanessa Ferreira da Silva; Loyola, Wagner; Kaneno, Ramon

doi:10.1590/s0074-02762008005000011

Cited by 14 publications

(13 citation statements)

References 42 publications

(39 reference statements)

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“…Although its role on the production of cytokines such as MCP-1 and TNF-α is well established, we observed no relationship between MR and TNF-α production [30, 31]. We also have previously demonstrated that treatment of mice with ATF increases phagocytosis and killing of C. albicans by murine macrophages, and this effect was associated with higher expression of MR [15]. One possible explanation for this difference is that monocytes used in this study and macrophages used in the murine investigation represent two different stages of cell differentiation and, therefore, can show different behavior after interaction with different ATF components.…”

Section: Discussionmentioning

confidence: 77%

“…We have previously observed that the acid-treated polysaccharide fraction of A. brasiliensis (ATF) can increase the resistance of mice against C. albicans by stimulating fungicidal activity of peritoneal macrophages, characterized by increased levels of H 2 O 2 , and increased expression of mannose receptor [15]. Then, we asked whether human monocytes would also be stimulated by A. brasiliensis β-glucan.…”

Section: Introductionmentioning

confidence: 99%

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Agaricus brasiliensis polysaccharides stimulate human monocytes to capture Candida albicans, express toll-like receptors 2 and 4, and produce pro-inflammatory cytokines

Martins

Soares

Domeneghini

et al. 2017

J Venom Anim Toxins Incl Trop Dis

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Background Agaricus brasiliensis is a medicinal mushroom with immunomodulatory and antitumor activities attributed to the β-glucans presented in the polysaccharide fraction of its fruiting body. Since β-glucans enhance cellular immunoresponsiveness, in this study we aimed to evaluate the effect of an acid-treated polysaccharide-rich fraction (ATF) of A. brasiliensis on the ability of human monocytes to adhere/phagocyte C. albicans yeast cells, their expression of pattern recognition receptors and their ability to produce cytokines.MethodsAdhesion/phagocytosis of FITC-labeled C. albicans was evaluated by flow cytometry. Cells were incubated with specific fluorochrome-labeled antibodies for TLR2 and 4, βGR and MR and also evaluated by flow cytometry. Monocytes were cultured with ATF, and culture supernatants were collected for analysis of in vitro cytokine production by ELISA (TNF-α, IL-1β, IL-12 and IL-10).ResultsATF significantly increased the adherence/phagocytosis of C. albicans by monocytes and this was associated with enhanced expression of TLR2 and TLR4, while no effect was observed on βGR or MR. Moreover, expression of TLR4 and TLR2 was associated with higher levels of in vitro production of TNF-α and IL-1, respectively. Production of IL-10 was also increased by ATF treatment, but we found no association between its production and the expression of Toll-like receptors.ConclusionOur results provided us with evidence that A. brasiliensis polysaccharides affect human monocytes probably through the modulation of Toll-like receptors.

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Section: Discussionmentioning

confidence: 77%

Section: Introductionmentioning

confidence: 99%

Agaricus brasiliensis polysaccharides stimulate human monocytes to capture Candida albicans, express toll-like receptors 2 and 4, and produce pro-inflammatory cytokines

Martins

Soares

Domeneghini

et al. 2017

J Venom Anim Toxins Incl Trop Dis

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“…Bernardshaw, et al studies have demonstrated that agaricus blazei murill extract can kill pneumococcus 6B in mice [13]. The same research team gave mice agaricus blazei murill extract before the infection of bacterial sepsis in another study, the experimental results show that the agaricus blazei murill extract can significantly reduce the mortality of mice [14].…”

Section: Antifungalmentioning

confidence: 95%

“…It is worth noting that Kalac think the risk of heavy metal content exceeds bid in artificial cultivation of edible mushrooms is very low. Although the main heavy metals lead, mercury, picks, arsenic in edible mushrooms are higher than that of green plants, different species, different cultivation conditions, different harvest time, et al can all influence the edible mushrooms enrichment degree and the content of heavy metals [13].…”

Section: Status Of Heavy Metal Content In Edible Mushroomsmentioning

confidence: 99%

The Status of Edible Mushrooms Resource Development and Nutrition Evaluation in Sichuan Province, China

Tang¹,

You²,

Li³

et al. 2016

Proceedings of the 2016 International Conference on Biological Sciences and Technology

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Abstract. With the development of our agriculture, the utilization of edible mushrooms is more and more widely recognized. The resources of them are abundant in our country, especially in Sichuan. They contain not only rich nutrients, but also active substances such as polysaccharides, which with the biological activity of anti-tumor, anti-diabetic, antifungal and anti-viral. Though edible mushrooms have become the polar industry of Sichuan, still many problems concerning the food safety of edible mushrooms need to be solved. This paper conducts a clear exposition of rich edible mushrooms resources and problems existing in processing and utilization of edible mushrooms in Sichuan.

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“…After this period, the slides were washed with complete culture medium, dyed with May-Grünwald-Giemsa and analysed under light microscopy. Results were expressed as phagocytic index calculated as the average number of attached plus ingested yeasts per phagocytising cell multiplied by proportion of phagocytising cells (Martins et al, 2008).…”

Section: Aliquots (1 )10mentioning

confidence: 99%

Agaricus brasiliensismycelium and its polysaccharide modulate the parameters of innate and adaptive immunity

Navegantes

Albuquerque

Dalla-Santa

et al. 2013

Food and Agricultural Immunology

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The biological properties of Agaricus brasiliensis mainly include immunostimulating and antitumour activities. This study evaluated the immunomodulating effect of A. brasiliensis mycelium (LPB) and its exopolysaccharides (EPS) on immunological parameters, such as phagocytosis of Candida albicans and nitric oxide (NO) production by infected macrophages, splenocytes proliferation in response to C. albicans. It also assessed the effects of the intake of LPB on the number of lymphocytes in the lymph node and spleen of treated animals and the TNF-a production. It was showed that the LPB and EPS had opposite effects; LPB had antiproliferative action and also reduced the NO levels produced by macrophages. However, the EPS showed an immune-stimulating effect potentiating the splenocytes proliferation and NO production from macrophages after stimulation with C. albicans. In addition, it also protected the macrophages from death induced by yeast. These data confirmed the immunomodulatory properties of LPB and its polysaccharides.

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Polysaccharide-rich fraction of Agaricus brasiliensis enhances the candidacidal activity of murine macrophages

Cited by 14 publications

References 42 publications

Agaricus brasiliensis polysaccharides stimulate human monocytes to capture Candida albicans, express toll-like receptors 2 and 4, and produce pro-inflammatory cytokines

Agaricus brasiliensis polysaccharides stimulate human monocytes to capture Candida albicans, express toll-like receptors 2 and 4, and produce pro-inflammatory cytokines

The Status of Edible Mushrooms Resource Development and Nutrition Evaluation in Sichuan Province, China

Agaricus brasiliensismycelium and its polysaccharide modulate the parameters of innate and adaptive immunity

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