Taenia solium metacestode immunodominant peptides recognized by IgG antibodies in cerebrospinal fluid and serum paired samples from patients with active and inactive neurocysticercosis

Barcelos, Ivanildes Solange da Costa; Moura, Leandro Pains; Costa, Vinicius Paulino da; Ferreira, Marcelo Simão; Costa-Cruz, Júlia Maria

doi:10.1590/s0074-02762007005000084

Cited by 8 publications

(10 citation statements)

References 24 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…However, the explanation for the specific selection of reactive peptides to NC‐In sera may be derived from results presented elsewhere (41), which demonstrated that active and inactive NC patients sera presented differential protein profiles. The immunodominant bands of 47–52, 64–68 and 70 kDa were predominant when testing the NC‐Ac sera, while the 12‐ to 14‐ and 39‐ to 42‐kDa bands were not recognized by the NC‐In sera.…”

Section: Discussionmentioning

confidence: 99%

“…Other studies have also demonstrated the ability to distinguish active from inactive NC forms (13–16,41), suggesting that this is related to differential patterns of antigen’s epitope recognition in the two phases of the disease, and part of this antigenic variations may be attributed to protein conformation and glycosylation (33). In our study, preparation of antigen extracts and chicken immunization may have generated loss of protein conformation or differential protein modifications, and these alterations may have artificially created antigens and specific epitopes that are mainly recognized by patients’ sera with the inactive NC form.…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Specific phage‐displayed peptides discriminate different forms of neurocysticercosis by antibody detection in the serum samples

Manhani

Ribeiro

Cardoso

et al. 2011

Parasite Immunology

View full text Add to dashboard Cite

Neurocysticercosis (NC), caused by Taenia solium metacestode, infects the central nervous system and is a devastating parasitic infection. Diagnosis is based on symptoms, imaging, serology and epidemiology. Current markers present variable sensitivity and specificity, frequent cross-reactions and are not able to discriminate NC clinical forms. The aim of this study was to select mimotopes of T. solium metacestode antigens that may be used in NC immunodiagnosis, specifically to discriminate between active and inactive forms. A random peptide phage display library was screened against IgY from chickens immunized with total saline extract from T. solium metacestodes and validated against 110 serum samples, classified into active NC (18), inactive NC (22), cross-reactive parasitic diseases (40) and healthy controls (30). We have successfully selected seven peptides with significant immunoreactivity to IgG of NC patients, with sensitivity ranging from 95.5% to 100% to detect the inactive form and specificity varied from 85.7% to 94.3%. One phage-displayed peptide (Cc48) can be directly used as biomarker to distinguish inactive from active forms with an accuracy of 95.7%, and this novel mimotope may also be used as an auxiliary tool to neuroimaging tests and treatment follow-up.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Specific phage‐displayed peptides discriminate different forms of neurocysticercosis by antibody detection in the serum samples

Manhani

Ribeiro

Cardoso

et al. 2011

Parasite Immunology

View full text Add to dashboard Cite

show abstract

“…A total saline extract with 50 metacestodes of T. solium was prepared and the obtained extract was divided into aliquots of 200 μL, identified and stored at −20 °C until processing. Serum samples were submitted to the ELISA test in accordance with Barcelos et al 10 Some 200 μg of the total saline extract protein was applied by 10 × 8 cm gel and subjected to 12% polyacrylamide gel electrophoresis (SDS-PAGE) for separation of the peptides present in the antigenic extract and subsequent transfer to 0.45 μm pore size nitrocellulose membranes. After transfer, the nitrocellulose membranes were cut into 3 mm strips.…”

mentioning

confidence: 99%

“…The antigenic peptides recognized by IgG antibodies present in the reagent serum samples in the ELISA were analyzed by Western blotting (WB). 10 The criterion for positivity in WB adopted was the recognition of at least two of the peptides, 18, 24, 28–32, 39–42, 47–52, 64–68, and 70 kDa, in WB specific for cysticercosis. 10 The results obtained in the ELISA and WB were analyzed using two-way ANOVA and Fisher's exact tests with p ≤ 0.05 in the software GraphPad Prism version 5.0.…”

mentioning

confidence: 99%

“… 10 The criterion for positivity in WB adopted was the recognition of at least two of the peptides, 18, 24, 28–32, 39–42, 47–52, 64–68, and 70 kDa, in WB specific for cysticercosis. 10 The results obtained in the ELISA and WB were analyzed using two-way ANOVA and Fisher's exact tests with p ≤ 0.05 in the software GraphPad Prism version 5.0. The total saline extract of T. solium metacestodes was prepared for use in both tests and presented a protein concentration of 3000 μg/mL.…”

mentioning

confidence: 99%

See 1 more Smart Citation

Seroprevalence of human cysticercosis in Jataí, Goiás state, Brazil

Guarda

Costa-Cruz

Barcelos

2018

The Brazilian Journal of Infectious Diseases

Self Cite

View full text Add to dashboard Cite

The Taenia solium cysticercosis affects millions of people worldwide and is considered a public health problem, especially in developing countries. The diagnosis of neurocysticercosis is complex and involves the analysis of epidemiological, clinical, neuroimaging, and immunological host data. Neurocysticercosis is endemic in Brazil, and is related to the cause of death mainly in the Southeast, South, and Central-West regions. The objective of this study was to determine the seroprevalence of cysticercosis in inhabitants of the city of Jataí, Goiás, in the Central-West region of Brazil from April to August 2012. A total of 529 serum samples were analyzed by enzyme-linked immunosorbent assay for detecting IgG antibodies against T. solium larvae, and Western blotting was used for confirming the diagnosis through the recognition of at least two specific peptides from their serum antibodies. The 351/529 (66.3%) reactive samples were analyzed by enzyme-linked immunosorbent assay and Western blotting confirmed the diagnosis in 73 samples that recognized at least two of the following peptides specific IgG antibodies for cysticercosis: 18, 24, 28-32, 39-42, 47-52, 64-68, and 70kDa. The seroprevalence of cysticercosis was 13.8% (95% CI 5.9-21.7), demonstrating that the studied area is endemic to this disease.

show abstract

Saline extract of Taenia saginata metacestodes as an alternative antigen for the immunodiagnosis of neurocysticercosis in human cerebrospinal fluid

et al. 2009

View full text Add to dashboard Cite

The aim of the present research was to test the application of Taenia saginata metacestodes as an alternative antigen for use in enzyme-linked immunosorbent assay (ELISA) and Western Blotting (WB) tests compared with the use of metacestodes antigen of Taenia solium in cerebrospinal fluid (CSF) samples. The samples were obtained from 35 patients with definitive neurocysticercosis (NCC)-group 1-and 44 patients with other neurological disorders (control)-group 2. The sensitivity and specificity of ELISA, using antigen obtained from T. solium, applied to the patients of group 1 yielded results of 100%. When the tests were conducted using T. saginata metacestodes, results were 100% and 93.2%, respectively. The 47-52-, 64-68-, and 70-kDa antigens showed high frequencies in CSF samples from patients with NCC when WB was conducted with both antigens. The results indicate that T. saginata metacestodes can be used as an alternative antigen for the diagnosis of human NCC in CSF samples.

show abstract

Taenia solium metacestode immunodominant peptides recognized by IgG antibodies in cerebrospinal fluid and serum paired samples from patients with active and inactive neurocysticercosis

Cited by 8 publications

References 24 publications

Specific phage‐displayed peptides discriminate different forms of neurocysticercosis by antibody detection in the serum samples

Specific phage‐displayed peptides discriminate different forms of neurocysticercosis by antibody detection in the serum samples

Seroprevalence of human cysticercosis in Jataí, Goiás state, Brazil

Saline extract of Taenia saginata metacestodes as an alternative antigen for the immunodiagnosis of neurocysticercosis in human cerebrospinal fluid

Contact Info

Product

Resources

About