Ribotyping and virulence markers of Yersinia pseudotuberculosis strains isolated from animals in Brazil

Abstract

Ribotyping and virulence markers has been used to investigate 68

A novel high-resolution melting analysis-based method for Yersinia pseudotuberculosis genotyping

“…The strains that were grouped into the "A" and "B" HRMA groups belonged, respectively, to sequence type (ST) 42 and 19 in the MLST scheme. However, when comparing our data with the ribotypes that were obtained by Martins et al (2007), no clusters related to specific Y. pseudotuberculosis bio-serogroups were observed. These authors typed a set of 68 Y. pseudotuberculosis strains using the ribotyping method and we used 40 of the 68 strains in the current study (Fig.…”

“…It is interesting to note that when information about the virulence markers that were found in the Y. pseudotuberculosis strains searched by Martins et al (2007) was added to the dendrogram that was obtained in the present study (Fig. 2), it was possible to identify distinct pathogenic potentials among the Y. pseudotuberculosis strains.…”

Molecular typing of Yersinia pseudotuberculosis strains isolated from livestock in Brazil

“…Despite the fact that several reports regarding the genotypic diversity of Yersinia species, including Y. pseudotuberculosis strains isolated around the world have been published, the molecular epidemiology of Y. pseudotuberculosis strains isolated from livestock in Brazil remains unclear (Kim et al, 2003;Voskressenskaya et al, 2005;Martins et al, 2007;Souza et al, 2010;Ch'ng et al, 2011;Laukkanen-Ninios et al, 2011).…”

“…Moreover, there were good correlations between the ERIC-PCR, PFGE, and MLST clusters and the pathogenic potential of the strains tested. According to Martins and Falcão (2004) and Martins et al (2007), the Y. pseudotuberculosis strains belonging to the 1/O:1a bio-serogroup (clustered in the ST 42, ERIC-A, and PFGE-A profiles) harbor more virulence genes and are more invasive than the Y. pseudotuberculosis strains belonging to the 2/O:3 bio-serogroup (clustered in the ST 19, ERIC-B, and PFGE-B profiles). These authors searched for the presence of virulence genes and investigated the kinetics of infection in mice for a set of 68 Y. pseudotuberculosis strains isolated in Brazil, from which we selected the 40 strains that were studied in this research.…”

“…To our knowledge, only two studies concerning the molecular epidemiology of Y. pseudotuberculosis strains isolated in this country have been published (Martins et al, 2007;Souza and Falcão, 2012). In the study of Martins et al (2007), ribotyping was performed but was not able to establish any epidemiological correlation between the strains. In the study of Souza and Falcão (2012), the high-resolution melting analysis technique grouped the strains according to their bio-serogroups.…”