The neuraminidase gene is present in the non-toxigenic Vibrio cholerae Amazonia strain: a different allele in comparison to the pandemic strains

Figueiredo, Sonia Catarina de Abreu; Neves-Borges, Anna Cristina; Coelho, Ana

doi:10.1590/s0074-02762005000600010

Cited by 6 publications

(2 citation statements)

References 30 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Podem sobreviver em condições ambientais desfavoráveis por um período de tempo: o estado VNC (Viável, mas Não Cultivável), sugerindo esse processo como uma adaptação ambiental em regiões como altos níveis de salinidade, temperatura e pH 16 19 , a flexibilidade da aquisição da expressão gênica para a produção de toxina da cólera foi percebida pelo processo de transferência horizontal (conjugação), que é o processo de troca genética entre procariotas. Contribuindo mais para esta discussão, Rivera et al 20 demonstrou um grande potencial de aquisição patogênica em indivíduos dos sorogrupos não O1 e não O139.…”

Section: Introductionunclassified

Acesso ao Saneamento básico e Incidência de Cólera: uma análise quantitativa entre 2010 e 2015

2019

View full text Add to dashboard Cite

RESUMO As condições de saneamento básico são um dos principais determinantes nos índices de agravos à saúde humana, principalmente no tocante a doenças infectocontagiosas por veiculação hídrica. Dentre todas, destacamos neste trabalho a cólera: doença entérica causada pelas cepas toxigênicas do Vibrio cholerae (sorogrupos O1 e O139), que é caracterizada por um quadro de acentuada diarreia. O objetivo deste trabalho, por meio de levantamento de dados mundiais de Incidência de Cólera (IC) e percentual populacional de Acesso ao Saneamento (AS), é quantificar, evidenciar e discutir a relação entre esses dois indicadores no período de 2010 a 2015, assim como apontar outros possíveis fatores magnificantes de vulnerabilidade socioambiental para a patologia. No período analisado, foram registrados 1.575.168 casos da doença, com as maiores incidências na região do subcontinente áfrico-asiático e em países isolados nos demais continentes, como, por exemplo, Haiti (América) e Papua Nova Guiné (Oceania), que também apresentaram sérios problemas de acessibilidade a serviços de saneamento. Desta forma, apesar de sugerirmos a análise de outros fatores socioambientais, as condições de saneamento revelaram-se como um determinante expressivo para a incidência da cólera em todas as regiões do mundo.

show abstract

Section: Introductionunclassified

Acesso ao Saneamento básico e Incidência de Cólera: uma análise quantitativa entre 2010 e 2015

2019

View full text Add to dashboard Cite

show abstract

“…19 Many studies point out the problem of horizontal transfer, a common process in some bacteria. 9 In this case, Vibrio cholera has great potential for the acquisition of toxigenic genes from non-O1 strains (non-toxigenic types) in interaction with O1 strains (toxigenic types), [20][21][22] as well as to develop microbial resistance. 20,23 The disease is one of the main public health problems in developing countries.…”

mentioning

confidence: 99%

Comparative Analysis of Spatial Distribution of Cholera by Temporal Fragments from 2000 to 2018

Silva¹,

Costa²,

Fernandez³

2022

Journal of Health Care for the Poor and Underserved

View full text Add to dashboard Cite

Cholera is caused by V .cholerae (serogroups O1 and O139) and has a close relationship with socio-environmental conditions, especially basic sanitation. Analytical methodologies for collecting quantitative data on health indicators are widely used to support epidemiological surveillance actions in human health. This work aims to verify the fluctuation of the spatial distribution of cholera in the world, by temporal fragments, between 2000 and 2018. During the analyzed period, approximately 5.3 billion cases with 75.6 thousand deaths occurred, with emphasis on the regions of Africa, South Asia, and the Caribbean, showing that the disease is still a serious public health problem, especially in populations in precarious situations. Although the methodology employed in this study presents practical obstacles of underreporting and integration between the different collection platforms, it is an important tool in the construction of health surveillance measures for infectious diseases.

show abstract

High Production of Neuraminidase by a Vibrio cholerae Non-O1 Strain—the First Possible Alternative to Toxigenic Producers

Еneva

Engibarov

Petrova

et al. 2015

Appl Biochem Biotechnol

View full text Add to dashboard Cite

Vibrio cholerae neuraminidase (VCNA) is widely used in biochemical and medical research, in processes for preparing homogenous sialoconjugates, and in the pharmaceutical industry. Its production by non-toxigenic strains is quite desirable, in order to avoid the expensive safety measures. Here, we report the first method for highly effective production of a novel, purified V. cholerae extracellular neuraminidase from a non-toxigenic strain. The enzyme is highly active, and its properties, as well as the responsible gene nanH, are practically identical with those of the toxigenic strains. It cleaves α,2 → 3 and α,2 → 6 glycosidic bonds with highest affinity (K M 1.7 × 10(-5) μM) for human transferrin. The deduced amino acid sequence of the enzyme reveals three binding sites for Ca(2+) and one for sialic acid. The sequence analysis of the nanH gene, being the first for a V. cholerae non-O1 strain, shows 99% identity with a new nanH allele of an O1 Vibrio strain. The simple laboratory technology for efficient production of the new VCNA is based on the use of common and cheap nutrient media and easily available inducer--glycomacropeptide. The rapid purification consists of salting-out and diethylaminoethanol (DEAE) and Q-Sepharose chromatography steps. Purified preparation contains no aldolase and protease, which gives the production scheme a great potential for industrial application.

show abstract

The neuraminidase gene is present in the non-toxigenic Vibrio cholerae Amazonia strain: a different allele in comparison to the pandemic strains

Abstract: The neuraminidase gene, nanH, is present

Cited by 6 publications

References 30 publications

Acesso ao Saneamento básico e Incidência de Cólera: uma análise quantitativa entre 2010 e 2015

Acesso ao Saneamento básico e Incidência de Cólera: uma análise quantitativa entre 2010 e 2015

Comparative Analysis of Spatial Distribution of Cholera by Temporal Fragments from 2000 to 2018

High Production of Neuraminidase by a Vibrio cholerae Non-O1 Strain—the First Possible Alternative to Toxigenic Producers

Contact Info

Product

Resources

About