Hepatitis C virus genotypes in blood donors from the Federal District, Central Brazil

Abstract

The objective of this study was to characterize hepatitis C virus (HCV)

Analysis of sample‐to‐cutoff ratios on chemiluminescent immunoassays used for blood donor screening highlights the need for serologic confirmatory testing

“…The data presented in this study demonstrated that high s/co ratios on the PRISM HCV ChLIA were predictive of confirmed‐positive results in our donor population, a finding that has previously been observed with other anti‐HCV screening IAs 1‐14 . A similar correlation between high s/co ratios and confirmed‐positive results was also found for the PRISM HBsAg, HIV O Plus, and HTLV‐I/II ChLIAs.…”

Detection of hepatitis C virus in patients with terminal renal disease undergoing dialysis in southern Brazil: prevalence, risk factors, genotypes, and viral load dynamics in hemodialysis patients

“…In our study, co-infections of the different genotypes were not identified. It has already been shown that genotype 1a is found in significant percentages in Brazil [10,33], although da Silva et al have identified the prevalence of genotype 1b followed by genotype 2b [12]. Further studies will be performed throughout southern Rio Grande do Sul to assess the distribution of the genotypes in dialysis units in southern Brazil.…”

“…In a study performed with hemodialysis patients in the city of Goiás, Espirito-Santo identified genotypes 1a (65.7%), 1b (26.7%) and 3 (7.6%) to be the most prevalent [33]. In a study of blood donors from Distrito Federal, Amorim et al identified the prevalence of genotypes 1a (82.3%), 3a (9.8%), and 1b (5.9%), followed by the co-infection of 1a/1b (2%) [10]. In our study, co-infections of the different genotypes were not identified.…”

“…Brazil is a continent-sized country, and although the prevalence of HCV among hemodialysis patients is known, with rates varying from 8.4% to 39.2%, the genotyping is not well documented [8]. Genotype 1 is predominant among hemodialyzed patients, and subtype 1a is the most frequently identified subtype, followed by 1b and 3a [8-10]. Two studies, one from Belo Horizonte, MG and a more recent study from Rio Grande/RS, showed that subtype 2b is the second most prevalent subtype [11,12].…”

Importância dos testes sorológicos de triagem e confirmatórios na detecção de doadores de sangue infectados pelo vírus da hepatite C

“…No Brasil, estudos realizados no Distrito Federal e em Goiânia relataram índices de positividade no teste confirmatório em doadores com teste de ELISA reagente para hepatite C de 71,9% e 82,0% respectivamente. 17,18 Resultados falso-positivos nas técnicas imunoenzimáticas são moderadamente freqüentes e podem ser devido ao clearance do vírus nos casos de cura espontânea (cicatriz sorológica), à presença de anticorpos contra proteínas residuais do vetor empregado na produção do antígeno recombinante que compõe o kit sorológico, às doenças auto-imunes e, ainda, à degradação protéica de soros estocados por longos períodos e/ou inadequadamente. 19,20 A porcentagem de resultados falso-positivos está inversamente relacionada à prevalência de infecção na população estudada.…”