Complete nucleotide sequence analysis of a Brazilian dengue virus type 2 strain

Santos, Flávia Barreto dos; Miagostovich, Marize Pereira; Nogueira, Rita Maria Ribeiro; Edgil, Dianna; Schatzmayr, Hermann G.; Riley, Lee W.; Harris, Eva

doi:10.1590/s0074-02762002000700011

Cited by 15 publications

(9 citation statements)

References 25 publications

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“…Phylogenetic analysis of DENV-2 strains circulating at that time confirmed the genotype circulating in Southeast Asia [26], [27]. This observation was further corroborated in an extensive analysis of viruses from the states of Rio de Janeiro (1990 and 1995), Ceará (1994), Bahia (1994 and 1999), Maranhão (1996 and 1998), Mato Grosso (1997), Pará (1998), Rio Grande do Norte (1998), Paraíba (1999) Sergipe (1999), Espiríto Santo (1995 and 2000) and forty strains isolated in Pernambuco (1995–2002) [28], [29].…”

Section: Discussionmentioning

confidence: 62%

Twenty Years of DENV-2 Activity in Brazil: Molecular Characterization and Phylogeny of Strains Isolated from 1990 to 2010

et al. 2013

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In Brazil, dengue has been a major public health problem since its introduction in the 1980s. Phylogenetic studies constitute a valuable tool to monitor the introduction and spread of viruses as well as to predict the potential epidemiological consequences of such events. Aiming to perform the molecular characterization and phylogenetic analysis of DENV-2 during twenty years of viral activity in the country, viral strains isolated from patients presenting different disease manifestations (n = 34), representing six states of the country, from 1990 to 2010, were sequenced. Partial genome sequencing (genes C/prM/M/E) was performed in 25 DENV-2 strains and full-length genome sequencing (coding region) was performed in 9 strains. The percentage of similarity among the DENV-2 strains in this study and reference strains available in Genbank identified two groups epidemiologically distinct: one represented by strains isolated from 1990 to 2003 and one from strains isolated from 2007 to 2010. No consistent differences were observed on the E gene from strains isolated from cases with different clinical manifestations analyzed, suggesting that if the disease severity has a genetic origin, it is not only due to the differences observed on the E gene. The results obtained by the DENV-2 full-length genome sequencing did not point out consistent differences related to a more severe disease either. The analysis based on the partial and/or complete genome sequencing has characterized the Brazilian DENV-2 strains as belonging to the Southeast Asian genotype, however a distinction of two Lineages within this genotype has been identified. It was established that strains circulating prior DENV-2 emergence (1990–2003) belong to Southeast Asian genotype, Lineage I and strains isolated after DENV-2 emergence in 2007 belong to Southeast Asian genotype, Lineage II. Furthermore, all DENV-2 strains analyzed presented an asparagine (N) in E390, previously identified as a probable genetic marker of virulence observed in DHF strains from Asian origin. The percentage of identity of the latter with the Dominican Republic strain isolated in 2001 combined to the percentage of divergence with the strains first introduced in the country in the 1990s suggests that those viruses did not evolve locally but were due to a new viral Lineage introduction in the country from the Caribbean.

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Section: Discussionmentioning

confidence: 62%

Twenty Years of DENV-2 Activity in Brazil: Molecular Characterization and Phylogeny of Strains Isolated from 1990 to 2010

et al. 2013

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“…The changes in envelope could affect immunogenicity or cell entry [14]. If there were a lot of changes in envelope of derivative, it might hard entry the cell.…”

Section: Resultsmentioning

confidence: 99%

Comparison of full-length genomics sequences between dengue virus serotype 3, parental strain, and its derivatives, and B-cell epitopes prediction from envelope region

Thaisonthi

Rabablert²,

Yoksan³

2013

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Biological markers are normally used to evaluate the candidate of live-attenuated dengue vaccines. D3V 16562 Vero 23 and D3V 16562 Vero 33 which were derivatives of D3V 16562, parental strain, showed the similar biological data. We used molecular techniques and computational tools to evaluate these derivatives. The nucleotide and amino acid sequences of the derivatives were compared to their parent. The secondary structures of untranslated regions and B-cell epitopes were predicted. The results showed that nucleotide substitutions mostly occurred in NS5 and NS5 of V2 was unusual because of amino acid change at 3349 (tryptophan →stop codon). The nucleotide substitutions in 5'UTR, prM, E, NS1, NS2A, NS3, and 3'UTR were 4, 1, 2, 2, 1, 3, and 2, respectively. The secondary structure of 5'UTR of V2 was different from P and V1. The secondary structure of 3'UTR of V2 was similar to P and certainly distinct from V1. Furthermore, B-cell epitopes prediction revealed that there were 21 epitopes of envelope and the interesting epitope was at position 297-309 because it was in domain III in which the neutralizing antibody is induced. For this study, the attenuation of derivatives was caused by the nucleotide substitutions in 5'UTR, 3'UTR, and NS5 regions. The genotypic data and B-cell epitope make the derivatives attractive for the chimeric and peptide DENV vaccine development.

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“…Recently, a nucleotide sequence of the entire genome from a Brazilian DEN-2 virus was determined for the first time, and a phylogenetic analysis also classified it into the Asian/American-Asian genotype (29). Nucleotide sequences of the entire genome were also determined recently from four Brazilian DEN-1 viruses (30).…”

Section: Discussionmentioning

confidence: 99%

Molecular epidemiology of type 1 and 2 dengue viruses in Brazil from 1988 to 2001

Neto

Lima

Paula

et al. 2005

Braz J Med Biol Res

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Complete nucleotide sequence analysis of a Brazilian dengue virus type 2 strain

Abstract: In the last decade, dengue fever (DF)

Cited by 15 publications

References 25 publications

Twenty Years of DENV-2 Activity in Brazil: Molecular Characterization and Phylogeny of Strains Isolated from 1990 to 2010

Twenty Years of DENV-2 Activity in Brazil: Molecular Characterization and Phylogeny of Strains Isolated from 1990 to 2010

Comparison of full-length genomics sequences between dengue virus serotype 3, parental strain, and its derivatives, and B-cell epitopes prediction from envelope region

Molecular epidemiology of type 1 and 2 dengue viruses in Brazil from 1988 to 2001

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