Twenty Years of DENV-2 Activity in Brazil: Molecular Characterization and Phylogeny of Strains Isolated from 1990 to 2010

Faria, Nieli Rodrigues da Costa; Nogueira, Rita Maria Ribeiro; Filippis, Ana María Bispo de; Simões, Jaqueline Bastos Santos; Nogueira, Fernanda de Bruycker; Lima, Monique da Rocha Queiroz; Santos, Flávia Barreto dos

doi:10.1371/journal.pntd.0002095

Cited by 35 publications

(39 citation statements)

References 45 publications

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“…Before sequencing, the 1957 isolate had been passaged in suckling mouse brain, but the earlier passage history is unclear; the 2011 isolate was passaged twice in Vero cells. This 2.2% amino acid divergence is within the range that has been observed for other flaviviruses with isolations ranging between 1 and 49 years apart, [17][18][19] indicating relatively little change in the ENTV genome in 54 years since the first isolation.…”

supporting

confidence: 73%

Detection of Entebbe Bat Virus After 54 Years

Kading

Kityo

Nakayiki

et al. 2015

The American Journal of Tropical Medicine and Hygiene

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Abstract. Entebbe bat virus (ENTV; Flaviviridae: Flavivirus), closely related to yellow fever virus, was first isolated from a little free-tailed bat (Chaerephon pumilus) in Uganda in 1957, but was not detected after that initial isolation. In 2011, we isolated ENTV from a little free-tailed bat captured from the attic of a house near where it had originally been found. Infectious virus was recovered from the spleen and lung, and the viral RNA was sequenced and compared with that of the original isolate. Across the polypeptide sequence, there were 76 amino acid substitutions, resulting in 97.8% identity at the amino acid level between the 1957 and 2011 isolates. Further study of this virus would provide valuable insights into the ecological and genetic factors governing the evolution and transmission of bat-and mosquitoborne flaviviruses.

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supporting

confidence: 73%

Detection of Entebbe Bat Virus After 54 Years

Kading

Kityo

Nakayiki

et al. 2015

The American Journal of Tropical Medicine and Hygiene

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“…The percentage identity of the latter with strain DR59/01 (Dominican Republic) isolated in 2001, combined with the percentage of divergence with strains first introduced in the 1990s, suggests a new viral lineage introduced from the Caribbean. No consistent differences were observed on the E gene from strains isolated from cases with different clinical manifestations [33]. …”

Section: Resultsmentioning

confidence: 99%

“…Intra-serotype antigenic variation and the resulting differential generation of protective antibodies and immune responses is postulated as one of the reasons for the high epidemiological impact of certain DENV serotypes in the Americas [60]. The lack of consistent differences in the E gene of Brazilian DENV-2 isolates from cases with different clinical manifestations suggests that if disease severity has a genetic basis, it is not solely due to differences observed in the E gene [33]. …”

Section: Discussionmentioning

confidence: 99%

Dengue in Latin America: Systematic Review of Molecular Epidemiological Trends

et al. 2017

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Dengue, the predominant arthropod-borne viral disease affecting humans, is caused by one of four distinct serotypes (DENV-1, -2, -3 or -4). A literature analysis and review was undertaken to describe the molecular epidemiological trends in dengue disease and the knowledge generated in specific molecular topics in Latin America, including the Caribbean islands, from 2000 to 2013 in the context of regional trends in order to identify gaps in molecular epidemiological knowledge and future research needs. Searches of literature published between 1 January 2000 and 30 November 2013 were conducted using specific search strategies for each electronic database that was reviewed. A total of 396 relevant citations were identified, 57 of which fulfilled the inclusion criteria. All four dengue virus serotypes were present and co-circulated in many countries over the review period (with the predominance of individual serotypes varying by country and year). The number of countries in which more than one serotype circulated steadily increased during the period under review. Molecular epidemiology data were found for Argentina, Bolivia, Brazil, the Caribbean region, Colombia, Ecuador, Mexico and Central America, Paraguay, Peru and Venezuela. Distinct lineages with different dynamics were found in each country, with co-existence, extinction and replacement of lineages occurring over the review period. Despite some gaps in the literature limiting the possibility for comparison, our review has described the molecular epidemiological trends of dengue infection. However, several gaps in molecular epidemiological information across Latin America and the Caribbean were identified that provide avenues for future research; in particular, sequence determination of the dengue virus genome is important for more precise phylogenetic classification and correlation with clinical outcome and disease severity.

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“…aegypti salivary glands 7 days earlier than in American genotype, suggesting a 2-to 65-fold increase in the vectorial capacity of mosquitoes [134]. It has been shown that Asiangenotype DENV strains causing DHF share a particular substitution in the envelope protein E-N390D, which seems to be involved in viral replication in macrophages and dendritic cells, and is thus considered to be a virulence marker [136,137]. Further studies should be conducted in order to elucidate the genetic determinants of SEA genotype fitness in mosquitoes.…”

Section: Dengue Virusmentioning

confidence: 99%

Arboviruses: Variations on an Ancient Theme

et al. 2014

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Arboviruses utilize different strategies to complete their transmission cycle between vertebrate and invertebrate hosts. Most possess an RNA genome coupled with an RNA polymerase lacking proofreading activity and generate large populations of genetically distinct variants, permitting rapid adaptation to environmental changes. With mutation rates of between 10 -6 and 10 -4 substitutions per nucleotide, arboviral genomes rapidly acquire mutations that can lead to viral emergence. Arboviruses can be described in seven families, four of which have medical importance: Togaviridae, Flaviviridae, Bunyaviridae and Reoviridae. The Togaviridae and Flaviviridae both have ssRNA genomes, while the Bunyaviridae and Reoviridae possess segmented RNA genomes. Recent epidemics caused by these arboviruses have been associated with specific mutations leading to enhanced host ranges, vector shifts and virulence. KEYWORDS Arboviruses: the role of RNA genomes in viral adaptation & emergenceThe recent emergence of many arboviruses (i.e., West Nile virus [WNV] in North America [1] and Chikungunya virus [CHIKV] in the Indian Ocean islands [2]) serves as a reminder that these viruses are capable of rapidly adapting to changes in their environment. Such viral epidemics can often be associated with the accumulation of one or more specific mutations in the viral genome and highlight a hallmark feature of arboviruses [3][4][5][6]. Indeed, arboviruses, and many other RNA viruses, employ a unique feature of their RNA-dependent RNA polymerases in order to achieve this rapid adaptation: inaccuracy due to a lack of proofreading activity [7][8][9]. This inaccuracy is the result of evolutionary pressure related to the fidelity of the polymerase. Mutations that decrease the fidelity of viral polymerases, have been shown to cause the accumulation of attenuating and lethal mutations [10], while mutations that enhance the fidelity of the polymerase are also associated with attenuation due to a reduction in the number of viral quasispecies, leaving the virus unable to cope with sudden environmental changes within the host [11]. These studies and others have shown that RNA virus replication balances on a 'knife's edge', with slight changes in their fidelity leading to population collapse [12]. Arboviruses are further constrained by the need to replicate in both a vector and a host species. These two very dissimilar organisms each place unique selective pressures on the arboviral genome, such that mutations that adapt the virus to favor one host are often deleterious in the other host [13][14][15]. Despite this evolutionary pressure, many arboviruses have expanded both their geographical distribution and host range through the generation and accumulation of beneficial mutations [16,17].The major source of these mutations comes from errors that occur during genome replication. Mutation rates during viral RNA replication are in the range of 10 -6 -10 -4 substitutions per For reprint orders, please contact: reprints@futuremedicine.com

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Twenty Years of DENV-2 Activity in Brazil: Molecular Characterization and Phylogeny of Strains Isolated from 1990 to 2010

Cited by 35 publications

References 45 publications

Detection of Entebbe Bat Virus After 54 Years

Detection of Entebbe Bat Virus After 54 Years

Dengue in Latin America: Systematic Review of Molecular Epidemiological Trends

Arboviruses: Variations on an Ancient Theme

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