Quantitave and qualitative interferences of pentoxifillyne on hepatic Schistosoma mansoni granulomas: effects on extracellular matrix and eosinophil population

Reis, Luciana Maria dos; T, Ventura; Souza, Sônia Oliveira; Arana-Pino, Arturo; Pelajo-Machado, Marcelo; Pereira, Mário; Lenzi, Henrique Leonel; Conceição, Maria José; Takiya, Christina Maeda

doi:10.1590/s0074-02762001000900015

Cited by 14 publications

(16 citation statements)

References 28 publications

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“…It was suggested by the authors that this lack of effect on the collagen network was probably due, in part, to the beginning of drug administration after granuloma formation, when immune cells were already stimulated. The results provided by El-Lakkany & Nosseir (2007) are similar to those of Reis et al (2001); however, the larger daily dose of PTX (200 mg/kg) used in the present study may explain the notable effects on the constitution of granulomatous lesions, even in short-term treatment with PTX and after the stimulation of schistosomotic granuloma development. A decrease in the total number of hepatic granulomas was observed, related to some toxic effect of the drug on egg release and/or adult worm fecundity.…”

Section: Discussionsupporting

confidence: 83%

“…Besides, the evaluation of second-stage immature S. mansoni eggs by the oogram technique utilized in the present work seems to be the most sensitive parameter to analyse alterations in the fecundity of the female parasite, since it permits the observation of newly laid eggs and shows few confounding factors, as suggested in a previous study (Pacheco et al, 2005). No change in the parameter second stage of immature egg/female worm/g and in total number of parasite eggs was observed, indicating the absence of alterations in egg viability and also in S. mansoni oviposition (table 2), in contrast to the studies of Reis et al (2001) and El-Lakkany & Nosseir (2007), who observed a reduction in egg number in rodent liver, as already mentioned.…”

Section: Discussionmentioning

confidence: 68%

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Effects of pentoxifylline duringSchistosoma mansoniinfection in Swiss mice: an analysis of worm burden, fecundity and liver histopathology

2010

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The short-term effects of pentoxifylline (PTX) on granulomatous lesions during Schistosoma mansoni infection in Swiss mice were evaluated. Drug administration was initiated 42 and 140 days post-infection (DPI) for the acute and chronic infection groups, respectively. Treatment was carried out daily with 200 mg/kg (subcutaneous route) of the drug for five consecutive days. Recovery of parasites and tissues was performed at 49 DPI and 147 DPI, respectively. Liver histological analysis showed a decrease in the inflammatory reaction and fibrous content of the granulomas studied, and a significant reduction (P < 0.001) in their mean diameter was observed in the groups of rodents treated with PTX in acute and chronic infection, when compared to their respective control groups. However, no alteration in the number of S. mansoni recovered from the portal system was observed, and egg-laying kinetics was not notably modified by PTX treatment, and the immature stage distribution of S. mansoni eggs showed minor intrinsic variations with no statistical differences in the parameter second-stage/female/g among untreated mice and treated mice in acute and chronic infections, respectively, when evaluated by intestinal oograms. Data obtained indicate probable immunomodulatory effects of PTX in murine schistosomiasis both in acute and chronic infection.

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Section: Discussionsupporting

confidence: 83%

Section: Discussionmentioning

confidence: 68%

Effects of pentoxifylline duringSchistosoma mansoniinfection in Swiss mice: an analysis of worm burden, fecundity and liver histopathology

2010

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“…Without doubt, major alterations arising out of PTX administration in rodents continue to be those observed previously in the liver (Reis et al, 2001;Xiong et al, 2003;El-Lakkany & Nosseir, 2007;Mati et al, 2010;Rabia et al, 2010). The liver is the organ where the immunological modulation process and its respective alterations on schistosomotic granulomata are more evident (Andrade & Warren, 1964;Carvalho et al, 1986;Silva et al, 2000;Tristão et al, 2000).…”

Section: Current Uses For Oograms In Experimental Schistosomiasismentioning

confidence: 66%

Current applications of oogram methodology in experimental schistosomiasis; fecundity of femaleSchistosoma mansoniand egg release in the intestine of AKR/J mice following immunomodulatory treatment with pentoxifylline

Mati

Melo

2012

J. Helminthol.

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AbstractOogram methodology permitted a direct assessment of the fecundity ofSchistosoma mansoniand the passage of parasite eggs from mesenteric vessels into the intestinal lumen in AKR/J mice that had been infected via the intraperitoneal route with 60 cercariae of the trematode and later subjected to short-term subcutaneous treatments with pentoxifylline (PTX). The administration of PTX did not change oviposition kinetics, the individual fecundity of female parasites (as determined by the study of the ratio of second-stageS. mansonieggs per g intestine/number of female parasites recovered from the portal system), nor the number of mature and dead eggs retained in the host tissue, though the drug has known immunomodulatory effects, as shown previously in experimental schistosomiasis. A better appraisal was also carried out, including the study of statistical parameters, concerning the utilization of the ratios of each stage of immature eggs (first to fourth) per g intestine/number of female worms from the portal system. The second-stage eggs had the lowest variability, confirming that the utilization of this stage as an indicator of the individual fecundity of parasite females is indeed viable. In the light of our findings, current uses of oogram methodology are discussed. Moreover, additional consideration is given to data obtained in the present study concerning intraperitoneal infection withS. mansonicercariae in both untreated and treated mice of the AKR/J strain, such as the recovery of mature worms, eggs and free granulomas from the peritoneal cavity of these rodents.

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“…Our study is topical and relevant as many of these techniques are commonly used (Vermaelen et al, 2003, Reis et al, 2001, and Hirasawa et al, 2007); however, documentation regarding their comparative usefulness for eosinophil detection in murine tissues is lacking.…”

Section: Discussionmentioning

confidence: 99%

Comparison of Histochemical Methods for Murine Eosinophil Detection in an RSV Vaccine-enhanced Inflammation Model

et al. 2009

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A comparative study of histochemical detection of eosinophils in fixed murine tissue is lacking. Five histochemical methods previously reported for eosinophil detection were quantitatively and qualitatively compared in an established murine RSV vaccine-enhanced inflammation model. Nonspecific neutrophil staining was evaluated in tissue sections of neutrophilic soft tissue lesions and bone marrow from respective animals. Eosinophils had granular red to orange-red cytoplasmic staining, depending on the method, whereas neutrophils had, when stained, a more homogenous cytoplasmic pattern. Nonspecific background staining of similar coloration was variably seen in vascular walls and erythrocytes. Astra Blue/Vital New Red, Congo Red, Luna, Modified Hematoxylin and Eosin, and Sirius Red techniques were all effective in detecting increased eosinophil recruitment compared to controls; however, differences in eosinophil quantification varied significantly between techniques. Astra Blue/Vital New Red had the best specificity for differentiating eosinophils and neutrophils but had a reduced ability to enumerate eosinophils and was the most time intensive. The Luna stain had excessive nonspecific staining of tissues and a reduced enumeration of infiltrating eosinophils, which made it suboptimal. For multiple parameters such as eosinophil detection, specificity, and contrast with background tissues, the Sirius Red followed by Congo Red and Modified Hematoxylin and Eosin methods were useful, each with their own staining qualities.

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Quantitave and qualitative interferences of pentoxifillyne on hepatic Schistosoma mansoni granulomas: effects on extracellular matrix and eosinophil population

Abstract: Mast cells and eosinophils actively participate in tissue repair and are prominent components of

Cited by 14 publications

References 28 publications

Effects of pentoxifylline duringSchistosoma mansoniinfection in Swiss mice: an analysis of worm burden, fecundity and liver histopathology

Effects of pentoxifylline duringSchistosoma mansoniinfection in Swiss mice: an analysis of worm burden, fecundity and liver histopathology

Current applications of oogram methodology in experimental schistosomiasis; fecundity of femaleSchistosoma mansoniand egg release in the intestine of AKR/J mice following immunomodulatory treatment with pentoxifylline

Comparison of Histochemical Methods for Murine Eosinophil Detection in an RSV Vaccine-enhanced Inflammation Model

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