Ultrastructural features of the midgut epithelium of females Lutzomyia intermedia (Lutz &amp; Neiva, 1912) (Diptera: Psychodidae: Phlebotominae)

Andrade-Coêlho, Cláudia A.; Santos-Mallet, Jacenir Reis dos; Souza, Nataly A.; Lins, Ulisses; Meirelles, M. N. L.; Rangel, Elizabeth Ferreira

doi:10.1590/s0074-02762001000800020

Cited by 20 publications

(13 citation statements)

References 35 publications

(48 reference statements)

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“…The presence of microvilli along the midgut epithelial cells observed in C. megacephala larvae corresponds with similar findings reported in other insects such as the sand flies, L. intermedia (Andrade-Coêlho et al 2001) and L. longipalpis (Leite and Evangelista 2001;Secundino et al 2005); fruit fly, Drosophila auraria (Dimitradis 1991); mosquitoes, A. (Stegomyia) aegypti (Zieler et al 2000;Moncayo et al 2005), and Anopheles darlingi (Okuda et al 2005); tick, H. longicornis (Matsuo et al 2003); beetle, Dendroctonus valens (Silva-Olivares et al 2003); bee, Melipona quadrifasciata anthidioides (Neves et al 2003); or the wingless firebrat, Thermobia domestica (Rost et al 2005).…”

Section: Discussionsupporting

confidence: 90%

“…To date, many researchers have reported the ultrastructure of various alimentary organs of numerous groups of arthropods. These include mosquitoes, Aedes (Stegomyia) aegypti (Zieler et al 2000;Moncayo et al 2005), Culex quinquefasciatus (Sais et al 2003); sand flies, Lutzomyia longipalpis (Secundino et al 2005), Lutzomyia intermedia (Andrade-Coêlho et al 2001); bot fly, Dermatobia hominis (Evangelista and Leite 2003); black fly, Simulium pertinax (Cavados et al 2004); fruit fly, Bactrocera dorsalis (Lee et al 1998;Hung et al 2000); ticks, Haemaphysalis longicornis (Matsuo et al 2003), Ixodes ricinus (Jasik and Buczek 2005), Rhipicephalus (Boophilus) microplus (Nunes et al 2005); mite, Platytrombidium fasciatum (Shatrov 2005); ants, Tetraponera (Billen and Buschinger 2000), Solenopsis saevissima (Arab and Caetano 2002), Pachycondyla villosa (Zara and Caetano 2003); reduviid bug, Triatoma infestans (Reis et al 2003); beetles, Dendroctonus (Silva-Olivares et al 2003), Odontotaenius disjunctus (Nardi et al 2006); and a moth, Hofmannophila pseudospretella (Gerard 2002). In the present study, observations of the morphology of the alimentary canal of C. megacephala larvae were carried out to the ultrastructural level to clarify their morphological composition and better understand the functional role of each associated organ.…”

Section: Introductionmentioning

confidence: 99%

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Fine structure of the alimentary canal of the larval blow fly Chrysomya megacephala (Diptera: Calliphoridae)

et al. 2006

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Morphology of the alimentary canal of the mature third instar larva of the blow fly, Chrysomya megacephala (F.), was examined using light, scanning, and transmission electron microscopy. Salivary structures consist of a single median deferent duct that bifurcates into efferent ducts connected to paired, tubular salivary glands comprised of closely packed conical-shaped epithelial cells with large nuclei. The crop occurs as a large, swollen diverticulum of the digestive tube and is lined internally with convoluted cuticle (epicuticle and endocuticle). The esophagus is a simple, straight tube internally lined with cuticle and externally encompassed by muscle fibers. The cardia is a bulb-like structure composed of anterior foregut tissue and posterior midgut tissue from which the peritrophic membrane (PM) is produced. The midgut begins within the cardia which is flanked posteriorly by four tubular gastric caeca that are lined internally with four to five layers of cuboidal epithelial cells bearing microvilli. Midgut tissue is lined with simple cuboidal epithelium whose cells are filled with numerous secretory granules and possessed long microvilli facing the lumen. A peritrophic membrane is contained within the midgut lumen. The larval hindgut consists of the pylorus, Malpighian tubules, ileum, colon, rectum, and anus, posteriorly. The pylorus is characterized by a single layer of epithelial cells encircled by a muscular layer and the presence of PM within the lumen. Malpighian tubules each diverge into two tubular structures totalling four long tubules of long chained cuboidal cells bearing microvilli internally. The wall of the ileum is comprised primarily of a monolayer of cuboidal epithelial cells with large oval nuclei and more intense muscular fibers surrounding the periphery. A cuticular layer surrounds the lumen containing the PM. This inner cuticle consists of a thin epicuticle that is electron-dense; whereas, the endocuticle is much thicker but less electron-dense. Myo-epithelial cells are dense in the anal region, where the PM persists.

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Section: Discussionsupporting

confidence: 90%

Section: Introductionmentioning

confidence: 99%

Fine structure of the alimentary canal of the larval blow fly Chrysomya megacephala (Diptera: Calliphoridae)

et al. 2006

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“…Morphometric studies of insect midgut epithelial cells are rare, especially those concerning the distinct metamorphosis phases of the midgut. Most of the studies involve ultrastructural analysis in insect orders such as Diptera (Nopanitaya & Misch 1974, Wood & Lehane 1991, Andrade-Coelho et al 2001, Hemiptera (Billingsley 1988, Ranjini & Mohamed 2000 and Lepidoptera (Cioffi 1979, Lello et al 1984, Santos et al 1984.…”

Section: Discussionmentioning

confidence: 99%

Morphometry of the midgut of Melipona quadrifasciata anthidioides (Lepeletier) (Hymenoptera: Apidae) during metamorphosis

Cruz¹,

Araujo²,

Dolder³

et al. 2011

Neotrop. entomol.

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In Hymenoptera, midgut changes begin in the last instar. At this stage, the larval epithelial digestive cells degenerate, leaving only the basal membrane and the regenerative cells which will develop into a new epithelium during the pupal stage and in the adult. Epithelium renewal is followed by changes in volume and shape of the midgut. Morphometric analysis of digestive cells and total midgut volume of Melipona quadrifasciata anthidioides (Lepeletier) were conducted to verify whether cell volume increase are sufficient to account for the total midgut volume increase that occurs during metamorphosis. An increase in midgut volume was verified in spite of the scarcity of cell proliferation found during metamorphosis. At the end of metamorphosis, the increase in cell volume was not sufficient to explain the increase in volume of the midgut, indicating that an increase in the number of digestive cells is apparently necessary. Nevertheless, the mechanism by which regenerative cells reconstitute the epithelium during metamorphosis remains unknown.

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“…The majority of studies involves ultrastructural analysis in insect orders such as Diptera (Nopanitaya and Misch, 1974;Wood and Lehane, 1991;Andrade-Coelho et al, 2001), Heteroptera (Ranjini and Mohamed, 2000), Hemiptera (Billingsley, 1988), and Lepidoptera (Cioffi, 1979;Lello et al, 1984;Santos et al, 1984). However, there is not a single study related to the resistance of insects to entomopathogens.…”

Section: Discussionmentioning

confidence: 99%

“…Some authors have demonstrated morphometric analyses in Diptera (Nopanitaya and Misch, 1974;Wood and Lehane, 1991;Andrade-Coelho et al, 2001), Heteroptera (Ranjini and Mohamed, 2000), Hemiptera (Billingsley, 1988) and Lepidoptera (Pinheiro et al, 2003), but none of them is related with resistance to entomopathogens in insects.…”

Section: Introductionmentioning

confidence: 99%

A morphometric study of the midgut in resistant and non-resistant Anticarsia gemmatalis (Hübner) (Lepidoptera: Noctuidae) larvae to its nucleopolyhedrovirus (AgMNPV)

Levy

Moscardi

Falleiros

et al. 2009

Journal of Invertebrate Pathology

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Ultrastructural features of the midgut epithelium of females Lutzomyia intermedia (Lutz & Neiva, 1912) (Diptera: Psychodidae: Phlebotominae)

Cited by 20 publications

References 35 publications

Fine structure of the alimentary canal of the larval blow fly Chrysomya megacephala (Diptera: Calliphoridae)

Fine structure of the alimentary canal of the larval blow fly Chrysomya megacephala (Diptera: Calliphoridae)

Morphometry of the midgut of Melipona quadrifasciata anthidioides (Lepeletier) (Hymenoptera: Apidae) during metamorphosis

A morphometric study of the midgut in resistant and non-resistant Anticarsia gemmatalis (Hübner) (Lepidoptera: Noctuidae) larvae to its nucleopolyhedrovirus (AgMNPV)

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