A morphometric study of the midgut in resistant and non-resistant Anticarsia gemmatalis (Hübner) (Lepidoptera: Noctuidae) larvae to its nucleopolyhedrovirus (AgMNPV)

Levy, Sheila Michele; Moscardi, F.; Falleiros, Ângela Maria Ferreira; Silva, Reinaldo J.; Gregório, Elisa Aparecida

doi:10.1016/j.jip.2009.02.010

Cited by 7 publications

(6 citation statements)

References 33 publications

(43 reference statements)

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“…In larvae treated with BRS 257 (control), the midgut wall presented an epithelial layer, covered by a peritrophic membrane and surrounded by a muscular layer, similar to described by several authors in others Lepidopteran species (CHAPMAN, 2013;LEVY et al, 2004;LEVY et al, 2009;SOUSA et al, 2009).…”

Section: Discussionsupporting

confidence: 74%

“…Another significant result noted was the fragmentation the peritrophic membrane of midgut of larvae fed with BR16, Dowling and PI229358 genotypes and/or its absence in those fed with the IAC 100 and PI 227687 genotypes. This membrane consists of a fine network composed of chitin, glycosaminoglycans, glycoproteins and proteins (LEVY et al, 2004;LEVY et al, 2009;TERRA, 2001). It has a role in preventing mechanical injuries to the MI epithelial cells and responsible for the compartmentalization of digestion and protection against microorganisms and toxins ingested along with food (TERRA, 2001).…”

Section: Discussionmentioning

confidence: 99%

“…The action of these substances begins when the caterpillar ingests the soybean leaves; the digestion occurs on the digestive tract, especially on the midgut, the principal region of the absorption of food and chemical-biological substances (CHAPMAN, 2013;TERRA;FERREIRA, 1991). Levy et al (2004Levy et al ( , 2009) and Levy (2005), have studied the internal morphology of digestive tract of the A. gemmatalis for years, especially with insects infected with nucleopolyhedrovirus AgMNPV, but there are no reports of the acting of flavonoids in midgut epithelium. Thus, the objective of the present study was to investigate the alterations caused by soybean genotypes with different rutin and genistin concentrations on the midgut epithelium of these insects.…”

Section: Introductionmentioning

confidence: 99%

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Post-ingestive effects of flavonoids in the midgut epithelium of Anticarsia gemmatalis (Hübner 1818) (Lepidoptera: Erebidae) larvae

Cruz

Hoffmann-Campo

Levy

et al. 2017

Semin. Cienc. Biol. Saude

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Um controle alternativo para a lagarta da soja tem sido o uso de plantas resistentes a insetos que contém flavonoides fenólicos. O intestino médio é a principal via de acesso do alimento e substâncias químicas ingeridas. O presente estudo examinou as alterações morfológicas no epitélio do intestino médio da lagarta da soja, após a ingestão de genótipos de soja contendo os flavonoides rutina e genistina. As lagartas e os genótipos (BRS 257 - controle, BR 16, Dowling, PI 229358, IAC 100 e PI 227687) foram obtidos do laboratório de criação de insetos da Embrapa Soja. Os intestinos médios foram coletados, fixados em Karnovsky, processados e analisados ao microscópio de luz. Todos os tratamentos causaram alterações nas células epiteliais do intestino médio. As alterações foram mais assíduas nas células colunares do que nos demais tipos celulares. Essas apresentaram grande quantidade de protrusões citoplasmáticas e de vacúolos. As células caliciformes apresentaram poucas alterações para todos os genótipos testados, enquanto as células regenerativas apresentaram alterações principalmente nos tratamentos Dowling e PI 227687. A membrana peritrófica estava ausente para os genótipos IAC100 e PI 227687. Conclui-se que os genótipos Dowling e PI 227687 foram bastante efetivos e promoveram grandes alterações morfológicas no intestino médio das lagartas da soja, podendo ser bastante eficaz para o controle desta praga.

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Section: Discussionsupporting

confidence: 74%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Post-ingestive effects of flavonoids in the midgut epithelium of Anticarsia gemmatalis (Hübner 1818) (Lepidoptera: Erebidae) larvae

Cruz

Hoffmann-Campo

Levy

et al. 2017

Semin. Cienc. Biol. Saude

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“…The significant increase in the length of the midgut, as found for M. quadrifasciata anthidioides, has already been reported in Hemiptera (Billingsley 1988) and Lepidoptera (Pinheiro et al 2003, Pinheiro & Gregório 2006, Levy et al 2009. It is known that in stingless bees, the increase in midgut size during the larval stage is due to the growth of digestive cells (Cruz-Landim & Mello 1970), and to the increase in cell number due to proliferation and differentiation of regenerative cells (Serrão & CruzLandim 2000, Martins et al 2005.…”

Section: Discussionmentioning

confidence: 73%

Morphometry of the midgut of Melipona quadrifasciata anthidioides (Lepeletier) (Hymenoptera: Apidae) during metamorphosis

Cruz¹,

Araujo²,

Dolder³

et al. 2011

Neotrop. entomol.

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In Hymenoptera, midgut changes begin in the last instar. At this stage, the larval epithelial digestive cells degenerate, leaving only the basal membrane and the regenerative cells which will develop into a new epithelium during the pupal stage and in the adult. Epithelium renewal is followed by changes in volume and shape of the midgut. Morphometric analysis of digestive cells and total midgut volume of Melipona quadrifasciata anthidioides (Lepeletier) were conducted to verify whether cell volume increase are sufficient to account for the total midgut volume increase that occurs during metamorphosis. An increase in midgut volume was verified in spite of the scarcity of cell proliferation found during metamorphosis. At the end of metamorphosis, the increase in cell volume was not sufficient to explain the increase in volume of the midgut, indicating that an increase in the number of digestive cells is apparently necessary. Nevertheless, the mechanism by which regenerative cells reconstitute the epithelium during metamorphosis remains unknown.

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“…For example, honey bees infected by Paenibacillus larvae showed a total disruption of the PM allowing bacteria to access the midgut epithelial cells (Garcia-Gonzalez & Genersch, 2013). However, whether pathogens are successful in overcoming the PM, other midgut properties such as epithelial thickness (or integrity) may impair the pathogen's ability to invade and cause systemic infection (Levy et al, 2009;Buchon et al, 2013).…”

Section: Discussionmentioning

confidence: 99%

Density‐dependent prophylaxis in primary anti‐parasite barriers in the velvetbean caterpillar

Silva

Serrão

Elliot

2016

Ecological Entomology

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1. Organisms rely on a set of primary barriers to prevent invasion by parasites, and secondary defences to fight parasites that breach the primary barriers. However, maintaining these defences to be active and effective is costly. Thus, hosts increase investment in anti-parasite defences under situations of high risk of infection and reduce defences when the risk is reduced (the 'Density-Dependent Prophylaxis' hypothesis).2. In the present study, it was tested whether the midgut primary defences of the velvetbean caterpillar Anticarsia gemmatalis Hübner present density-dependent plasticity, and also whether these defences could be induced by a viral pathogenic challenge. The aim was to examine whether morphometry and the structure of the midgut and peritrophic matrix (PM) change in accordance with colour transition in caterpillars, and whether such changes may provide the caterpillars a more protective barrier against invasion by Anticarsia gemmatalis multiple nucleopolyhedrovirus (AgMNPV).3. It was found that PM and the midgut epithelium of the velvetbean caterpillar change plastically according to phenotype, itself a response to changes in population density. Caterpillars reared at high densities (black phenotype) had a considerably thicker midgut epithelia and peritrophic matrices than those reared individually (green phenotype), and there was also more chitin in the PM of the former.4. This was interpreted as the first demonstration of increased investment in primary, barrier, defences against parasites, in response to increased conspecific density and an increased risk of infection. The possibility that this arises as a positive result of pleiotropy is discussed further, wherein the biochemical pathways responsible for the up-regulation of the immune system are also involved in midgut properties.

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A morphometric study of the midgut in resistant and non-resistant Anticarsia gemmatalis (Hübner) (Lepidoptera: Noctuidae) larvae to its nucleopolyhedrovirus (AgMNPV)

Cited by 7 publications

References 33 publications

Post-ingestive effects of flavonoids in the midgut epithelium of Anticarsia gemmatalis (Hübner 1818) (Lepidoptera: Erebidae) larvae

Post-ingestive effects of flavonoids in the midgut epithelium of Anticarsia gemmatalis (Hübner 1818) (Lepidoptera: Erebidae) larvae

Morphometry of the midgut of Melipona quadrifasciata anthidioides (Lepeletier) (Hymenoptera: Apidae) during metamorphosis

Density‐dependent prophylaxis in primary anti‐parasite barriers in the velvetbean caterpillar

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