A comparative study of preservation and storage of Haemophilus influenzae

Saab, Olga C Aulet de; Castillo, Marta C. de; Holgado, A. Pesce De Ruiz; Nader, Olga M. de

doi:10.1590/s0074-02762001000400022

Cited by 27 publications

(13 citation statements)

References 7 publications

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“…Isolates were identified using standard biochemical tests such as the oxidase test, growth and lactose fermentation in MacConkey and triple sugar iron agar, the MRVP test, and growth in Simmons' citrate agar (9). All isolates were stored at -80°C in tryptic soy broth containing 40% glycerol (10).…”

Section: Bacterial Isolatesmentioning

confidence: 99%

Identification of Extended-Spectrum β-Lactamase Genes and AmpC-β-Lactamase in Clinical Isolates of Escherichia coli Recovered from Patients with Urinary Tract Infections in Kerman, Iran

Koshesh

Mansouri

Hashemizadeh

et al. 2016

Arch Pediatr Infect Dis

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Background: Escherichia coli is the main causative pathogen in urinary tract infections (UTIs). Antibiotic resistance in this bacterium is an important problem in public health. Objectives: The aim of this study was to identify the blaTEM, blaSHV, blaOXA, and blaPER genes and AmpC-β-lactamase in clinical isolates of E. coli recovered from patients with UTIs in Kerman, Iran. Methods: E. coli isolates (N = 105) were analyzed for their antibiotic susceptibility with the disk diffusion method. ESBL and AmpCproducing isolates were detected using phenotypic methods. PCR was used to identify the blaTEM, blaSHV, blaOXA and blaPER genes in ESBL and AmpC-positive isolates. Results: More than 50% of the isolates were multi-drug resistant. The prevalence of ESBLs, AmpC-β-lactamase, blaTEM and blaOXA in the inpatient isolates was 37.2%, 2%, 37.2% and 5.8%, respectively. Further, the prevalence of ESBLs, blaTEM, blaSHV and blaOXA in the outpatient isolates was 42.5%, 24%, 5.5% and 1.8%, respectively. Conclusions: The prevalence of ESBL-producing E. coli strains in the community (outpatients) is higher than that in inpatients in Kerman, Iran. An outbreak of ESBL-producing isolates in the community can be a serious problem for public health, as resistance to other classes of antibiotics such as aminoglycoside and fluoroquinolones is often related with ESBL and AmpC production, therefore, detection of ESBL and AmpC-producing isolates in the community and hospitals is very important for the treatment and prevention of such isolates.

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Section: Bacterial Isolatesmentioning

confidence: 99%

Identification of Extended-Spectrum β-Lactamase Genes and AmpC-β-Lactamase in Clinical Isolates of Escherichia coli Recovered from Patients with Urinary Tract Infections in Kerman, Iran

Koshesh

Mansouri

Hashemizadeh

et al. 2016

Arch Pediatr Infect Dis

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“…Alternatively, skim milk, a common freeze-drying protectant (Barbaree et al, 1982), can be used as a freezing solution (Takahashi et al, 1982, Essiain & Flournoy, 1986, Sinha et al, 1974, Hasegawa et al, 1967, as is regularly used in our laboratory to store Pseudomonas aeruginosa. A literature review indicates that the predominant studies of cyroprotectants focus on sensitivity to freeze/thaw cycles (Takahashi et al, 1982, Aulet de Saab et al, 2001 with few comparisons of viability over time at elevated temperature (Gruft et al, 1968, Essiain & Flournoy, 1986. While recovering our bacterial strains from 31 days at ambient temperatures (in this case, sustained temperatures of ∼30°C) due to power loss as a result of hurricanes Katrina and Rita, we observed an increase in recovery of strains that were stored in 10% skim milk versus 15% glycerol.…”

Section: Introductionmentioning

confidence: 72%

Skim milk enhances the preservation of thawed −80 °C bacterial stocks

Cody

Wilson

Hendrixson

et al. 2008

Journal of Microbiological Methods

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The results from bacterial strain recovery efforts following hurricanes Katrina and Rita are reported. Over 90% of strains frozen in 10% skim milk were recovered whereas various recovery rates were observed for glycerol-stored stocks (56% and 94% of E. coli, depending upon the laboratory). These observations led to a viability comparison of Streptococcus pyogenes, Campylobacter jejuni, Borrelia burgdorferi, Salmonella enterica subsp. Typhimurium, Pseudomonas aeruginosa and Escherichia coli strains stored in glycerol or skim milk. In all bacteria examined, 10% skim milk resulted in significantly longer viability after thawing than 15% glycerol solutions currently used in most laboratories.

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“…Isolates were cultured on Haemophilus test medium, confi rmed as H. infl uenzae using standard methodology and stored as glycerol stock until further use. [8,9] capB gene of H. infl uenzae is known as virulence gene as Type b capsular polysaccharide is a major virulence factor and target for serum antibodies. Isolates with Type b serotype from both invasive and commensal isolates were detected through capB polymerase chain reaction (PCR) as described earlier.…”

Section: Methodsmentioning

confidence: 99%

Multi locus sequence type comparison of invasive and commensal Haemophilus influenzae isolates from Delhi

Saikia

Bewal

Bansal

et al. 2011

Indian Journal of Medical Microbiology

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Haemophilus influenzae is a major public health concern in the developing world. The most virulent strain is H. influenzae Type b (Hib). Hib also constitutes a major portion of nasopharyngeal commensal flora in otherwise healthy individuals. Through dendogram based on composite gene sequences of seven multi locus sequence type genes, it was observed that invasive and commensal isolates made two completely separate clusters which are indicative of independent evolution of these two groups of H. influenzae in the Indian subcontinent.

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A comparative study of preservation and storage of Haemophilus influenzae

Cited by 27 publications

References 7 publications

Identification of Extended-Spectrum β-Lactamase Genes and AmpC-β-Lactamase in Clinical Isolates of Escherichia coli Recovered from Patients with Urinary Tract Infections in Kerman, Iran

Identification of Extended-Spectrum β-Lactamase Genes and AmpC-β-Lactamase in Clinical Isolates of Escherichia coli Recovered from Patients with Urinary Tract Infections in Kerman, Iran

Skim milk enhances the preservation of thawed −80 °C bacterial stocks

Multi locus sequence type comparison of invasive and commensal Haemophilus influenzae isolates from Delhi

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