Relationship between biological behaviour and randomly amplified polymorphic DNA profiles of Trypanosoma cruzi strains

Martínez-Díaz, Rafael A.; Escario, José Antonio; Nogal-Ruiz, Juan José; Gómez‐Barrio, Alicia

doi:10.1590/s0074-02762001000200020

Cited by 7 publications

(5 citation statements)

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“…Cunha et al (2006) have reported significant reduction in parasites at the parasitaemic peak using an intraperitonal treatment of animals infected with the Y strain of T. cruzi with 2 mg/Kg/day of ursolic acid. In the present study, we demonstrate that the oral treatment with 20 mg/Kg/day of this compound is able to promote similar decrease in parasitaemia using a T. cruzi strain known as being more resistant to chemotherapy with benznidazole (Ribeiro et al 1988;Martinez-Díaz et al 2001). Furthermore, oral treatment has some advantages over intraperitonal treatment such as more facile administration and no discomfort due to injections.…”

Section: Resultssupporting

confidence: 61%

Trypanocidal activity and acute toxicity assessment of triterpene acids

et al. 2010

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The present study evaluates the in vitro and in vivo trypanocidal activity of ursolic acid and oleanolic acid against the Bolivia strain of Trypanosoma cruzi. Their acute toxicity is also assessed on the basis of median lethal dose (DL50) determination and quantification of biochemical parameters. Ursolic acid is the most active compound in vitro, furnishing IC50 of 25.5 microM and displaying 77% of trypomastigote lysis at a concentration of 128 microM. In agreement with in vitro assays, the results obtained for the in vivo assay reveals that ursolic acid (at a dose of 20 mg/Kg/day) provides the most significant reduction in the number of parasites at the parasitemic peak. Results concerning the LD50 assay and the biochemical parameters evaluated in the present study demonstrate that these substances can be safely used on an experimental basis.

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Section: Resultssupporting

confidence: 61%

Trypanocidal activity and acute toxicity assessment of triterpene acids

et al. 2010

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“…Although a reduced number of T. cruzi strains were analyzed, an interesting finding is the equal association among strains by their biological characteristics and their RAPD patterns. This correlation found between RAPD profiles and biological behavior of T. cruzi strains emphasizes its usefulness for studying parasite populations (Martínez-Díaz et al 2001). Genotypic analysis using RAPD and SSR-PCR amplified profiles demonstrated a large, genetically well-correlated group that contained the majority of T. cruzi strains and a divergent group including only one strain (Gomes et al 2003).…”

Section: Discussionmentioning

confidence: 78%

Random amplified polymorphic DNA profiles of Trypanosoma cruzi isolates from chagasic patients with different clinical forms

et al. 2006

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The genetic variability of 61 Trypanosoma cruzi isolates from 47 chronic chagasic patients of Minas Gerais state was analyzed by random amplified polymorphic DNA polymerase chain reaction (RAPD-PCR) using M13-40, lambdagt11-F, and L15996 primers. Cluster analysis by unweighted pair group method analysis was applied to RAPD profiles, and cluster analysis used to verify a possible correlation among different clinical forms of the disease from these patients. The T. cruzi isolates showed distinct grouping on tree topology, with the isolates not being possible to establish a correlation to the clinical forms of Chagas' disease. These data showed that the T. cruzi isolates from these patients would compose a group of populations well correlated genetically.

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“…Si bien Saravia et al (25), Márquez et al (26) y Ruiz et al (27), entre otros, han estudiado la variabilidad genética de un mayor número de cepas colombianas, estos estudios se han centrado en el análisis de los patrones isoenzimáticos, metodología superada por el RAPD para la determinación de polimorfismo y correlación con el comportamiento biológico de cepas del parásito (28).…”

Section: Discussionunclassified

Estudio de la variabilidad de seis cepas colombianas de Trypanosoma cruzi mediante polimorfismos de longitud de fragmentos de restricción (RFLP) y amplificación aleatoria de ADN polimórfico (RAPD).

Rodríguez¹,

Escalante²,

Díez³

et al. 2002

biomedica

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La enfermedad de Chagas, causada por el hemoflagelado Trypanosoma cruzi, constituye un problema de salud pública en Colombia en donde diferentes informes indican la presencia de heterogeneidad entre poblaciones del parásito. En este estudio se analizaron seis cepas colombianas de T. cruzi, procedentes de distintas regiones geográficas del país, huéspedes y ciclos de transmisión, mediante las técnicas de amplificación aleatoria de ADN polimórfico (Random Amplified Polymorphic DNA, RAPD), isoenzimas y polimorfismo de longitud de fragmentos de restricción (Restriction Fragment Length Polymorphisms, RFLP), usando como sonda el gen de 1,2 kb que codifica para la histona H2A del parásito. Se encontró que las distancias genéticas entre los aislados varían considerablemente, ubicándose en el rango de 0,61 a 0,99, para el caso de los perfiles de RAPD (cebadores M13F y M13R), de 0 a 0,81, para el caso de los perfiles de RFLP (5 endonucleasas) y de 0,10 a 0,55, para el caso de las isoenzimas (13 sistemas enzimáticos). El elevado grado de variabilidad exhibido por los aislados colombianos del parásito podría estar implicado en el amplio espectro clínico de presentación de la enfermedad de Chagas en Colombia.Palabras clave: Trypanosoma cruzi, RAPD, RFLP, histona H2A, isoenzimas, polimorfismo. Genetic variability in six Colombian Trypanosoma cruzi strains detected by restriction fragment length polymorphisms (RFLP) and random amplified polymorphic DNA (RAPD)Chagas disease, caused by the hemoflagellate Trypanosoma cruzi, is a public health problem in Colombia. Previous reports have indicated the presence of heterogeneity among parasite populations. Six Colombian T. cruzi strains were obtained that differed by host, geographical region and transmission cycle. The genetic variability of each was compared by random amplified polymorphic DNA (RAPD), and isoenzymes. A restriction fragment length polymorphism (RFLP) was extracted using the 1.2 kb unit encoding the parasite's H2A histone as a probe. Genetic distances between the isolates varied greatly, from 0.611 to 0.99 as determined by RAPD profiles (M13F and M13R primers), between 0 and 0.81 by RFLP profiles ( 5 endonucleases), and between 0.10 and 0.55 by isoenzymes (13 enzymatic systems). Genetic distance matrixes derived from each of the three methods showed that Colombian strains exhibit a high degree of genetic differentiation. This may account for the broad clinical spectrum of Chagas disease in Colombia.

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Relationship between biological behaviour and randomly amplified polymorphic DNA profiles of Trypanosoma cruzi strains

Cited by 7 publications

References 20 publications

Trypanocidal activity and acute toxicity assessment of triterpene acids

Trypanocidal activity and acute toxicity assessment of triterpene acids

Random amplified polymorphic DNA profiles of Trypanosoma cruzi isolates from chagasic patients with different clinical forms

Estudio de la variabilidad de seis cepas colombianas de Trypanosoma cruzi mediante polimorfismos de longitud de fragmentos de restricción (RFLP) y amplificación aleatoria de ADN polimórfico (RAPD).

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