Estudio de la variabilidad de seis cepas colombianas de Trypanosoma cruzi mediante polimorfismos de longitud de fragmentos de restricción (RFLP) y amplificación aleatoria de ADN polimórfico (RAPD).

Rodrí­guez, Pilar Rodriguez; Escalante, Marcela; Díez, Hugo; Cuervo, Claudia; Montilla, Marleny; Santiago, Nicholls Rubén; Zarante, Ignacio; Puerta, Concepción J.

doi:10.7705/biomedica.v22i3.1163

Cited by 5 publications

(3 citation statements)

References 37 publications

(34 reference statements)

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“…Generation of CD8 + T cell epitopes is influenced by several factors including abundance of the targeted protein, proteosome enzymatic cutting and peptide affinity for its respective MHC molecules (37). Even more, in the case of T. cruzi , the identification of specific CD8 + T cell epitopes could be hampered by the parasite genetic polymorphism (38) and proteomic complexity that includes at least 12 000 proteins gathered into super‐families (39,40).…”

Section: Discussionmentioning

confidence: 99%

“…proteosome enzymatic cutting and peptide affinity for its respective MHC molecules (37). Even more, in the case of T. cruzi, the identification of specific CD8 + T cell epitopes could be hampered by the parasite genetic polymorphism (38) and proteomic complexity that includes at least 12 000 proteins gathered into super-families (39,40). Our group has previously shown that the nonapeptide K1, located at the N-terminal T. cruzi KMP-11 protein is an HLA-A*0201 restricted epitope recognized by natural parasite exposed CD8 + T cells (16,21).…”

Section: Discussionmentioning

confidence: 99%

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Frequency of specific CD8+ T cells for a promiscuous epitope derived from Trypanosoma cruzi KMP-11 protein in chagasic patients

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et al. 2010

Parasite Immunology

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The K1 peptide is a CD8(+)T cell HLA-A*0201-restricted epitope derived from the Trypanosoma cruzi KMP-11 protein. We have previously shown that this peptide induces IFN-gamma secretion by CD8(+)T cells. The aim of this study was to characterize the frequency of K1-specific CD8(+)T cells in chagasic patients. Nineteen HLA-A2(+)individuals were selected from 50 T. cruzi infected patients using flow cytometry and SSP-PCR assays. Twelve HLA-A*0201(+)noninfected donors were included as controls. Peripheral blood mononuclear cells were stained with HLA-A2-K1 tetramer, showing that 15 of 19 infected patients have K1-specific CD8(+)T cells (0.09-0.34% frequency) without differences in disease stages or severity. Of note, five of these responders were A*0205, A*0222, A*0226, A*0259 and A*0287 after molecular typing. Thus, a phenotypic and functional comparison of K1-specific CD8(+)T cells from non-HLA-A*0201 and HLA-A*0201(+)infected patients was performed. The results showed that both non-HLA-A*0201 and HLA-A*0201(+)individuals have a predominant effector memory CD8(+)T cell phenotype (CCR7-, CD62L-). Moreover, CD8(+)T cells from non-HLA-A*0201 and HLA-A*0201(+)individuals expressed IL-2, IFN-gamma and perforin without any differences. These findings support that K1 peptide is a promiscuous epitope presented by HLA-A2 supertype molecules and is highly recognized by chagasic patients.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Frequency of specific CD8+ T cells for a promiscuous epitope derived from Trypanosoma cruzi KMP-11 protein in chagasic patients

Lasso

Mesa

Cuéllar

et al. 2010

Parasite Immunology

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“…Usando marcadores de isoenzimas, RAPDs y ADNr, se ha podido demostrar la mayor prevalencia del grupo T. cruzi I en el país (53)(54)(55)61). Con dos sistemas diferentes en genes codificadores de proteínas también fue posible en este estudio reproducir esta observación, agrupándose la mayoría de cepas del grupo I dentro de los genotipos TriR 3 y Crp 5; esta consistencia de agrupación con diferentes sistemas refuerza así la estructura clonal de las poblaciones de T. cruzi.…”

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Análisis de polimorfismos en los genes tripanotión reductasa y cruzipaína en cepas colombianas de Trypanosoma cruzi

et al. 2007

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Introducción. Los estudios genéticos en Trypanosoma cruzi han buscado establecer asociaciones de variantes genéticas del parásito con manifestaciones clínicas de la enfermedad, origen biológico y geográfico de los aislamientos; sin embargo, los resultados de asociación con los marcadores comúnmente usados en estos estudios han generado mucha controversia, principalmente en la asociación de grupos con características clínicas y epidemiológicas de la enfermedad. Objetivo. Se planteó determinar la variabilidad de genes que codifican para las proteínas tripanotión reductasa y cruzipaína, involucradas en mecanismos de infección y supervivencia del parásito en el hospedador mamífero, y probar la asociación de variantes génicas con origen biológico y geográfico de cepas colombianas de T. cruzi. Materiales y métodos. Se tipificaron por reacción en cadena de la polimerasa-polimorfismo de longitud del fragmento de restricción seis SNPs (Single Nucleotide Polymorphisms) en tripanotión reductasa y ocho SNPs en cruzipaína en 36 cepas colombianas de T. cruzi de diferentes regiones y origen biológico. Resultados. Con las enzimas Acy I y Hae III se determinaron tres genotipos para tripanotión reductasa. Para cruzipaína se identificaron seis genotipos con las enzimas Rsa I, Ban I y Bsu 36I. Conclusiones. Para tripanotión reductasa no fue posible establecer una asociación con el origen biológico o geográfico; sin embargo los alelos producidos en las posiciones 102 y 210, permitieron discriminar los grupos tradicionales I y II. Con los genotipos obtenidos para cruzipaína se establecieron relaciones a estos grupos, origen biológico y geográfico. Los resultados sugieren la utilidad de estos genes como marcadores moleculares para determinar y diferenciar variedades genéticas en T. cruzi.Palabras clave: Trypanosoma cruzi, genes protozoarios, polimorfismo, polimorfismo de longitud del fragmento de restricción, Colombia. Analysis of polymorphisms in the trypanothione reductase and cruzipain genes in Colombian strains of Trypanosoma cruziIntroduction. Genetic studies of Trypanosoma cruzi have tried to establish relations between genetic variants and their biological characteristics, such as clinical manifestations, host or geographic origin. However, much controversy exists on the associations between the commonly used DNA markers with group, clinical characteristics and disease epidemiology. Objective. In this study determined the variability of the genes that code for the proteins trypanothione reductase and cruzipain, both involved in the infection and survival of the parasite in the mammalian host, was studied and the association between genetic polymorphism and biological and geographic sources in Colombian T. cruzi strains was examined. Materials and methods. The genotypes for each of six SNPs (single nucleotide polymorphisms) for trypanothione reductase and eight SNPs for cruzipain genes were identified by polymerase chain reaction-restriction fragment length polymorphism.in 36 T. cruzi Colombian stocks from several regions and bi...

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ITS–RFLP- and RAPD-based genetic variability of Trypanosoma cruzi I, human and vector strains in Santander (Colombia)

et al. 2009

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Chagas disease is a severe public health problem in Latin-American countries. In Colombia, the predominance of Trypanosoma cruzi I has been described in the literature, with a broad heterogeneity between strains. However, most of the studies carried out centered on isoenzyme analysis, with a smaller number that focus on other molecular methods. In this report, we discuss the results of a molecular analysis of T. cruzi I strains, isolated from the domestic cycle, from the department of Santander, one of the territorial divisions where the prevalence of infection is highest. Internal transcribed spacer-restriction fragment length polymorphism and random amplification of polymorphic DNA were used to characterize 16 strains from human and vector (Triatominae) hosts. The data reveal a clustering based on the biological origin. Human and vector strains grouped separately; however, three vector strains clustered together with human strains. These results indicate that genetic differences exist in the strains that infect both hosts. We conclude that T. cruzi I populations in the domestic cycle of transmission of Chagas disease in Santander are heterogeneous and are composed of different clones. The epidemiological and biological implications are discussed.

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Estudio de la variabilidad de seis cepas colombianas de Trypanosoma cruzi mediante polimorfismos de longitud de fragmentos de restricción (RFLP) y amplificación aleatoria de ADN polimórfico (RAPD).

Cited by 5 publications

References 37 publications

Frequency of specific CD8+ T cells for a promiscuous epitope derived from Trypanosoma cruzi KMP-11 protein in chagasic patients

Frequency of specific CD8+ T cells for a promiscuous epitope derived from Trypanosoma cruzi KMP-11 protein in chagasic patients

Análisis de polimorfismos en los genes tripanotión reductasa y cruzipaína en cepas colombianas de Trypanosoma cruzi

ITS–RFLP- and RAPD-based genetic variability of Trypanosoma cruzi I, human and vector strains in Santander (Colombia)

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