Mutations in the rpoB gene of rifampicin-resistant Mycobacterium tuberculosis strains isolated in Brazil and France

Abstract

We evaluated the mutations in a 193bp of the rpoB gene by automated sequencing of rifampicin (RMP)-resistant and susceptible Mycobacterium tuberculosis strains isolated from Brazil (25 strains) and France (37 strains). In RMP-resistant strains, mutations were identified in 100% (16/16) from France and 89% (16/18) from Brazil. No mutation was detected in the 28 RMP-susceptible strains. Among RMP-resistant or RMP-susceptible strains deletion was observed. A double point mutation which had not been reported before was detected in one strain from France. Among French resistant strains mutations were found in codons 531 (31.2%), 526, 513 and 533 (18.7% each). In Brazilian strains the most common mutations were in codons 531 (72.2%), 526 (11.1%) and 513 (5.5%). The heterogeneity found in French strains may be related to the fact that most of those strains were from African or Asian patients

Fast test for assessing the susceptibility of Mycobacterium tuberculosis to isoniazid and rifampin by real-time PCR

“…This contrasts with previous data showing that RIF-resistant isolates in Brazil exhibited rpoB mutation frequencies varying from 80–89% (Valim et al 2000, de Miranda et al 2001, Clemente et al 2008). Previous data on katG reported that mutations occur in approximately 60–85% of INH-resistant isolates (Rossetti et al 2002, Cardoso et al 2004, Hofling et al 2005, Clemente et al 2008) but, in our study, mutation of the katG gene was seen in 55% (65/118) of the INH-resistant isolates.…”

Geographical Profile ofrpoBGene Mutations in Rifampicin ResistantMycobacterium tuberculosisIsolates in Sri Lanka

“…9,13,22,26,36,37 Mutations of codon 531, 526, and 516 have been reported as the most frequent mutations in the mutation hotspot region of the rpoB gene of MTB worldwide. 3,23,36 In the present study, the majority of mutations occurred at codon 526, and the mutation frequency (48.4%) observed was slightly higher than frequencies reported by China (40%), 36 Japan (35%), 16 and Korea (38%).…”

Review: The Molecular Basis of Resistance in <i>Mycobaterium tuberculosis</i>

“…In addition, mutations in codon 533 have been reported for RMP-resistant and RMP-susceptible strains by Taniguchi et al [74]. However, in studies conducted with isolates from same regions in Brazil and France, this mutation was strongly involved with RMP resistance [75].…”