Trypanosomes of non-human primates from the National Centre of Primates, Ananindeua, State of Pará, Brazil

Ziccardi, Mariangela; Lourenço‐de‐Oliveira, Ricardo; Lainson, Ralph; Brígido, Maria do Carmo de Oliveira; Muniz, José Augusto Pereira Carneiro

doi:10.1590/s0074-02762000000200004

Cited by 26 publications

(12 citation statements)

References 2 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…This trypanosome is a widely distributed species detected in several neotropical non-human primates, such as A. palliata , Saimiri sciureus , Saguinus midas , and Callithrix penicillata (Dunn et al., 1963, Souza and Dawson, 1976, Ziccardi and Lourenço de Oliveira, 1997, Ziccardi et al., 2000, Chinchilla et al., 2005, Sato et al., 2008). The transmission route of T. minasense is unknown.…”

Section: Discussionmentioning

confidence: 99%

“…(2008). The prevalence found in our study (96%) was much higher than that recorded in the updated bibliography of non-human primates; however, these reports were based on blood smear analyses, hemoculture and/or xenodiagnosis (De Resende et al., 1994, Ziccardi and Lourenço de Oliveira, 1997; Ziccardi et al., 2000). Our prevalence results showed similar values to those obtained by Sato et al.…”

Section: Discussionmentioning

confidence: 99%

“…In addition to two zoonotic trypanosome species, T. cruzi and T. rangeli , neotropical non-human primates, primarily species of the Cebidae family, can also be infected with T. (Megatrypanum) minasense (Chagas, 1908, Dunn et al., 1963, Hoare, 1972, Deane et al., 1974, De Resende et al., 1994, Ziccardi et al., 2000, Sato et al., 2008, Tenório et al., 2014). Regarding the genus Alouatta , trypomastigotes of T. minasense have been recorded by morphologic techniques in wild individuals of Alouatta palliata from Costa Rica (Chinchilla et al., 2005) and by molecular diagnosis in one captive A. caraya in the Centre for Wild Fauna (CCWF) from Brazil (Tenório et al., 2014).…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Molecular characterization of trypanosomatid infections in wild howler monkeys ( Alouatta caraya ) in northeastern Argentina

Martínez

Kowalewski

Salomón

et al. 2016

International Journal for Parasitology: Parasites and Wildlife

View full text Add to dashboard Cite

The transmission of Trypanosoma cruzi by vectors is confined to the Americas, and the infection circulates in at least two broadly defined transmission cycles occurring in domestic and sylvatic habitats. This study sought to detect and characterize infection by T. cruzi and other trypanosomes using PCR strategies in blood samples from free-ranging howler monkeys, Alouatta caraya, in the northeastern Argentina. Blood samples were collected at four sites with variable levels of habitat modification by human activity. PCR was conducted using primers for kinetoplast DNA, satellite DNA and ribosomal DNA of the trypanosomatid parasites. Ribosomal and satellite DNA fragments were sequenced to identify the trypanosomatid species and to characterize the discrete typing units (DTUs) of T. cruzi. Overall, 46% (50/109) of the howlers were positive according to the kDNA-PCR assay, but only 7 of the howlers were positive according to the SatDNA-PCR protocol. We sequenced the amplicons of the satellite DNA obtained from five specimens, and the sequences were 99% and 100% similar to T. cruzi. A sequence typical of DTU T. cruzi I was found in one howler monkey from the “remote” site, while sequences compatible with DTUs II, V, and VI were found in howlers from the “remote”, “rural” and “village” sites. We detected 96% positive samples for RibDNA-PCR, 9 of which were sequenced and displayed 99% identity with Trypanosoma minasense, while none showed identity with T. cruzi. The results demonstrated the presence of T. cruzi and a species closely related to T. minasense in blood samples from free-ranging A. caraya, belonging to different T. cruzi DTUs circulating in these howler monkey populations. The results obtained in this study could help evaluate the role of A. caraya as a reservoir of T. cruzi in regions where Chagas disease is hyper-endemic and where the human-wildlife interface is increasing.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Molecular characterization of trypanosomatid infections in wild howler monkeys ( Alouatta caraya ) in northeastern Argentina

Martínez

Kowalewski

Salomón

et al. 2016

International Journal for Parasitology: Parasites and Wildlife

View full text Add to dashboard Cite

show abstract

“…Trypanosomes have been commonly reported in the NHP families Callitrichidae (tamarins and marmosets), Cebidae (squirrel monkeys and capuchins), Aotidae (night monkeys), and Atelidae (spider monkeys and howler monkeys) (Sousa et al 1974; Ziccardi and Lourenco-de-Oliveira 1997; Ziccardi et al 2000; Maia da Silva et al 2008). …”

Section: Discussionmentioning

confidence: 99%

“…However, there is need for caution when comparing prevalences from different studies because of the different sampling techniques and the different diagnostic methods used (with varying sensitivities). For instance, the infection rates of T. cruzi in Amazonian monkeys ranged from 10.3% using parasitological methods (Ziccardi and Lourenco-de-Oliveira 1997; Ziccardi et al 2000) to 46% using serology (Lisboa et al 2006). Furthermore, in studies using PCR several other aspects must be taken into account, such as sample type, sample volume and conservation, DNA extraction method, parasite sequences targeted, primers, reagents, and thermocycling conditions (Luquetti and Schmuñis 2010).…”

Section: Discussionmentioning

confidence: 99%

Molecular Epidemiology of Trypanosomatids and Trypanosoma cruzi in Primates from Peru

Aysanoa

Mayor

Mendoza³

et al. 2017

EcoHealth

View full text Add to dashboard Cite

We determined the prevalence rate and risk of infection of Trypanosoma cruzi and other trypanosomatids in Peruvian non-human primates (NHPs) in the wild (n = 126) and in different captive conditions (n = 183). Blood samples were collected on filter paper, FTA cards, or EDTA tubes and tested using a nested PCR protocol targeting the 24Sα rRNA gene. Main risk factors associated with trypanosomatid and T. cruzi infection were genus and the human–animal context (wild vs captive animals). Wild NHPs had higher prevalence of both trypanosomatids (64.3 vs 27.9%, P < 0.001) and T. cruzi (8.7 vs 3.3%, P = 0.057), compared to captive NHPs, suggesting that parasite transmission in NHPs occurs more actively in the sylvatic cycle. In terms of primate family, Pitheciidae had the highest trypanosomatid prevalence (20/22, 90.9%) and Cebidae had the highest T. cruzi prevalence (15/117, 12.8%). T. cruzi and trypanosomatids are common in Peruvian NHPs and could pose a health risk to human and animals that has not been properly studied.

show abstract

Trypanosoma cruzi infection in captive Neotropical primates in the Brazilian Amazon

Bahia

Barros

Magalhães-Matos

et al. 2016

American J Primatol

View full text Add to dashboard Cite

The aim of this study was to detect the infection by Trypanosoma cruzi in captive Neotropical primates in the Brazilian Amazon. From February 2013 to July 2014, 112 blood samples were collected from Neotropical primates from the Amazonas, Amapá, and Pará States, north of Brazil. The subjects belonged to the families Cebidae (N = 59), Atelidae (N = 41), Callitrichidae (N = 5), Pitheciidae (N = 4), and Aotidae (N = 3). Blood smears also were examined for the presence of trypomastigotes by optical microscopy. For the detection of T. cruzi DNA, a Nested-PCR with primers TCZ1/TCZ2 and TCZ3/TCZ4 was performed. T. cruzi DNA was detected in 12.5% (14/112) of Neotropical primates examined. Positive samples were detected in 16%, 12.5%, and 11.11% of the different species of primates sampled from the Amapá, Pará, and Amazonas states, respectively. The analysis of the blood smears did not reveal trypomastigote forms of T. cruzi. In conclusion, Neotropical primates kept in captivity were infected by T. cruzi in the studied areas. We recommend that a health management protocol be put into place to prevent the transmission of infectious agents among captive populations, captive and wild populations, and between NHPs and the technicians who handle these animals.

show abstract

Trypanosomes of non-human primates from the National Centre of Primates, Ananindeua, State of Pará, Brazil

Cited by 26 publications

References 2 publications

Molecular characterization of trypanosomatid infections in wild howler monkeys ( Alouatta caraya ) in northeastern Argentina

Molecular characterization of trypanosomatid infections in wild howler monkeys ( Alouatta caraya ) in northeastern Argentina

Molecular Epidemiology of Trypanosomatids and Trypanosoma cruzi in Primates from Peru

Trypanosoma cruzi infection in captive Neotropical primates in the Brazilian Amazon

Contact Info

Product

Resources

About