Further studies on the molecular systematics of Biomphalaria snails from Brazil

Vidigal, Teofânia H. D. A.; Caldeira, Roberta Lima; Simpson, Andrew J.G.; Carvalho, Omar S.

doi:10.1590/s0074-02762000000100009

Cited by 64 publications

(64 citation statements)

References 21 publications

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“…Genomic DNA extractions form snails were performed using the DNeasy Blood & Tissue Kit (Qiagen), according to the protocol of Vidigal et al (2000). The purified genomic DNA was stored at -80°C until use.…”

Section: Dna Extractionmentioning

confidence: 99%

Digenetic larvae in Schistosome snails from El Fayoum, Egypt with detection of Schistosoma mansoni in the snail by PCR

et al. 2014

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The present study aims to detect the digenetic larvae infections in Bulinus truncatus and Biomphalaria alexandrina snails and also PCR detection of Schistosoma mansoni infection. The snails were collected from different branches of Yousef canal and their derivatives in El Fayoum Governorate. The snails were investigated for infection through induction of cercarial shedding by exposure to light and crushing of the snails. The shed cercariae were S. mansoni, Pharyngeate longifurcate type I and Pharyngeate longifurcate type II from B. alexandrina, while that found in B. truncatus were Schitosoma haematobium and Xiphidiocercaria species cercariae. The seasonal prevalence of infection was discussed. Polymerase chain reaction was used for the detection of S. mansoni in the DNA from field collected infected and non infected snails. The results of PCR showed that the pool of B. alexandrina snails which shed S. mansoni cercariae in the laboratory, gave positive reaction in the samples. Pooled samples of field collected B. alexandrina that showed negative microscopic shedding of cercariae gave negative and positive PCR in a consecutive manner. Accordingly, a latent infection in the snail (negative microscopic) could be detected by using PCR.

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Section: Dna Extractionmentioning

confidence: 99%

Digenetic larvae in Schistosome snails from El Fayoum, Egypt with detection of Schistosoma mansoni in the snail by PCR

et al. 2014

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“…A fragment from the cephalopodal region of the molluscs was collected for DNA extraction, using the Wizard Genomic DNA Purification Kit (Promega), as modified by Vidigal et al (2000). Ten specimens from Taim and 10 from Joinville populations, as well as 9 individuals from F1 generation and 16 from F2 generation were aleatory selected and were submitted to PCR-RFLP analysis.…”

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confidence: 99%

Dominant character of the molecular marker of a Biomphalaria tenagophila strain (Mollusca: Planorbidae) resistant to Schistosoma mansoni

Rosa¹,

Caldeira

Carvalho

et al. 2004

Mem. Inst. Oswaldo Cruz

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Biomphalaria tenagophila is a planorbid with a wide distribution in South America (Paraense 1984), and has epidemiological importance, since this species maintains the cycle of the trematode Schistosoma mansoni in some areas in Brazil. In spite of its dominance in some areas, B. tenagophila is found in the nature with low rates of infection, however, it is responsible for the majority of the autochthonous cases of schistosomiasis in the state of São Paulo, as well as for the foci of the disease in the states of Minas Gerais and Santa Catarina (Paraense 1986). This last focus is maintained by a highly susceptible population (Joinville) of molluscs to S. mansoni. On the other hand, there is a population coming from the Ecological Reservoir from Taim (state of Rio Grande do Sul), that has systematically been found resistant to different strains of S. mansoni (Coelho 1995). This population has been maintained under laboratory conditions for more than twenty years, without any selective pressure favoring the resistance. Vidigal et al. (1998), using the polymerase chain reaction and restriction fragment length polymorphism (PCR-RFLP) of the ribosomal RNA internal transcribed spacer region (ITS) with restriction DdeI enzyme, were able to separate molecularly the molluscs genus Biomphalaria, such as B. glabrata, B. tenagophila, and B. straminea, which are intermediate hosts of S. mansoni in Brazil. Those authors obtained species-specific profiles for this three species, observing besides that various Brazilian populations of B. tenagophila showed a characteristic profile, with two fragments (800 and 470 pb). Barbosa (2001), using the same technique, observed that the Taim population, in addition to the characteristic species-specific profile of B. tenagophila, as described by Vidigal et al. (1998), has a 350 pb fragment too.In the present study, cross-breedings between molluscs from B. tenagophila (Taim) and B. tenagophila (Joinville) were carried out, aiming at verifying whether the marker of the Taim population has a dominant character.Studies in order to obtain F1 generation were undertaken using a specimen of B. tenagophila Taim and another one of albino B. tenagophila Joinville. They were maintained together in order to achieve cross-breeding (4 pairs). The albino recessive character was used, in this case, as a phenotypic marker. The molluscs with 5 mm in diameter, approximately, sexually immature, were put together into a plastic recipient with 100 ml dechlorinated water for 50 days. Afterwards, the respective pairs were separately put into glasses in order to obtain F1 descendants. In this experiment, the egg-layings from the albino snails, as well as from the pigmented ones, were collected for four weeks. The egg-layings from F1 generation were transferred to an aquarium until the molluscs could reach 5 mm in diameter. F2 generation was obtained by means of cross-breedings between F1 individuals according to the same procedures previously described.A fragment from the cephalopodal region of the molluscs was col...

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“…The molluscs collected were sent to our laboratory to obtain their F1 progeny. Morphological and molecular identification of Biomphalaria was undertaken according to Paraense (1975Paraense ( , 1981Paraense ( , 1984 and Vidigal et al (2000) (Fig. 1).…”

mentioning

confidence: 99%

Biomphalaria molluscs (Gastropoda: Planorbidae) in Rio Grande do Sul, Brazil

Pepe

Caldeira²,

Carvalho³

et al. 2009

Mem. Inst. Oswaldo Cruz

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Further studies on the molecular systematics of Biomphalaria snails from Brazil

Abstract: The polymerase chain reaction and restriction fragment length polymorphism (RFLP)

Cited by 64 publications

References 21 publications

Digenetic larvae in Schistosome snails from El Fayoum, Egypt with detection of Schistosoma mansoni in the snail by PCR

Digenetic larvae in Schistosome snails from El Fayoum, Egypt with detection of Schistosoma mansoni in the snail by PCR

Dominant character of the molecular marker of a Biomphalaria tenagophila strain (Mollusca: Planorbidae) resistant to Schistosoma mansoni

Biomphalaria molluscs (Gastropoda: Planorbidae) in Rio Grande do Sul, Brazil

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