“…Thus, PCR-RFLP of the Internal Transcribed Spacer of the rDNA (ITS -which includes the 5.8S rDNA gene together with the flanking ITS1 and ITS2 spacers) was employed in the identification of 10 Brazilian species of Biomphalaria (Vidigal et al, , 2000a, of other South American and Cuban species of the same genus (Caldeira et al, 2000;Vidigal et al, 2001;Velásquez et al, 2002) and of morphologically similar species in the B. straminea and B. tenagophila complexes (Caldeira et al, 1998;Spatz et al, 1999), among others (Spatz et al, 2000). B. tenagophila guaibensis Paraense, 1984, B. oligoza Paraense, 1974and B. peregrina (Orbigny, 1835 of several localities in the state of Rio Grande do Sul were also identified using this technique (Pepe et al, 2009). Later, the molecular characterization of B. amazonica Paraense 1966 and B. cousini Paraense, 1966 was performed, and B. cousini was recorded in Brazil for the first time on the basis of PCR-RFLP of the ITS region, sequences of the 16S and ITS2 regions and morphology (Teodoro et al, 2010).…”