Profile of organic acid concentrations in the digestive gland and hemolymph of Biomphalaria glabrata under estivation

Bezerra, José Clecildo Barreto; Kemper, Andreas; Becker, W.

doi:10.1590/s0074-02761999000600012

Cited by 22 publications

(27 citation statements)

References 21 publications

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“…The larvae are located in the interfollicular tissue and are bathed by the haemolymph, from which they obtain the glucose needed for the glycogenesis process. They excrete lactate as the final substance of anaerobic metabolism (Bezerra et al 1999). the haemolymph (q = 23.161, p < 0.001) and glycogen in the cephalopedal mass (q = 6.903, p < 0.001) of infected B. glabrata in the second week with and without latex, but in the third week, with and without latex, there were no significant differences in parameters (glucose in haemolymph, q = 2.414, p > 0.05; glycogen in the digestive gland, q = 3.554, p > 0.05; glycogen in the cephalopedal mass, q = 4.223, p > 0.05).…”

Section: Discussionmentioning

confidence: 99%

“…105(4): 492-495, July 2010 Biomphalaria glabrata is the main intermediate host involved in schistosomiasis in Brazil. Studies have shown that physiological stress conditions, such as infection with Schistosoma mansoni, starvation, aestivation and exposure to molluscicides, can alter its carbohydrate metabolism (Schwartz & Carter 1982, Becker 1983, Bezerra et al 1999, Alcanfor 2001, Mello-Silva et al 2006a). These changes can alter the glycogenesis, gluconeogenesis and glycolysis in the snail.…”

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Carbohydrate metabolism alterations in Biomphalaria glabrata infected with Schistosoma mansoni and exposed to Euphorbia splendens var. hislopii latex

Mello-Silva¹,

Vilar²,

Vasconcellos

et al. 2010

Mem. Inst. Oswaldo Cruz

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Biomphalaria glabrata is the main intermediate host involved in schistosomiasis in Brazil. Studies have shown that physiological stress conditions, such as infection with Schistosoma mansoni, starvation, aestivation and exposure to molluscicides, can alter its carbohydrate metabolism (Schwartz & Carter 1982, Becker 1983, Bezerra et al. 1999, Alcanfor 2001, Mello-Silva et al. 2006a). These changes can alter the glycogenesis, gluconeogenesis and glycolysis in the snail.One of most promising Brazilian molluscicides is the crude extract of Euphorbia splendens (Sin. Euphorbia milii), which under laboratory and field conditions meets the recommendations of the WHO for use as a natural molluscicide (Vasconcellos & Amorim 2003). Mello-Silva et al. (2006a, 2007 studied the influence of sublethal doses of the latex of this plant on the carbohydrate and protein metabolism and reproductive biology of B. glabrata and found strong metabolic changes leading to a reduction in its population and consequently in the transmission of the parasite. In spite of this potential, there are no studies of the influence of this product on the physiology of Biomphalaria infected with S. mansoni.The present paper examines the action of the latex of E. splendens var. hislopii on the glucose content of the haemolymph and on the carbohydrate (glycogen) deposits of B. glabrata (BH strain) infected with S. mansoni (BH strain). MATERIALS AND METHODSObtaining the latex of E. splendens var. hislopiiSamples of E. splendens var. hislopii latex were collected in the autumn from plants cultivated in plots near the Biology Department, Fiocruz, in Rio de Janeiro, Brazil. The latex was collected as described by Vasconcellos and Amorim (2003) on the same day the tests were conducted.Lethal dose experiment -Using the recently collected latex, an aqueous stock solution at a concentration of 100 mg/L was prepared and, from this, solutions of different concentrations were prepared for use in the bioassays. The lethal (LC 90 ) and sublethal (LC 50 ) concentrations were determined according to Vasconcellos and Amorim (2003), as recommended by the World Health Organization (1983) and Mott (1987). The LC 50 and LC 90 values were, respectively, 1.0 mg/L and 2.3 mg/L (Mello-Silva et al. 2006a).Balloon flasks (1000 mL) were used and the latex solution was divided into two 500 mL glass beakers. The groups of B. glabrata (BH lineage), infected and uninfected, respectively, were placed in LC 50 solutions and exposed for 24 h (Vasconcellos & Amorim 2003) at 21ºC. Two glass beakers received 500 mL of distilled water without latex as a control group. None of the snails was fed during this period.After the latex exposure period, the snails were removed from the flasks and rinsed in distilled water to remove the residues. The number of dead specimens was noted. (1990). The snails were grouped according to their infection stage (1 day and 1, 2, 3 and 4 weeks post exposure). In each period analysed, 100 infected and 20 uninfected snails (control) were used. Sixty infected sn...

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Section: Discussionmentioning

confidence: 99%

mentioning

confidence: 99%

Carbohydrate metabolism alterations in Biomphalaria glabrata infected with Schistosoma mansoni and exposed to Euphorbia splendens var. hislopii latex

Mello-Silva¹,

Vilar²,

Vasconcellos

et al. 2010

Mem. Inst. Oswaldo Cruz

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“…The control group did not receive calcium carbonate in the water. At the intervals of 1, 14, 21 or 30 days, the hemolymph was collected from 15 snails (pool of five snails each, resulting in three samples) of each experimental group, according to Bezerra et al (1999). The boxes had the water and calcium carbonate concentrations renewed weekly.…”

Section: Composition Of the Study Group And Hemolymph Extractionmentioning

confidence: 99%

“…Then, 250 μL hemolymph was applied to the column. After application of the sample, 2mL of ultrapure water was added, the Bond Elut was removed from vacuum, 250 μL 0.5 M H 2 SO 4 was added, and the mixture was centrifuged at 500g/5minutes, 2 °C (Bezerra et al, 1999).…”

Section: Organic Acid Profilementioning

confidence: 99%

“…In aerobic conditions, it is possible to observe high concentrations of pyruvate, oxaloacetate, citrate, succinate, fumarate and malate in the snail hemolymph, which indicate the activation of the TCA cycle. In an alternative metabolic pathway, it is possible to find increased concentrations of ketone bodies, such as acetoacetate and beta-hydroxybutyrate, which are products of the lipid metabolism, while the increase in lactate, oxalate and propionate indicate anaerobic glycolytic processes (Bezerra et al, 1999;Tunholi et al, 2013).…”

Section: Introductionmentioning

confidence: 99%

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Changes in energetic metabolism of Biomphalaria glabrata (Mollusca, Planorbidae) in response to exogenous calcium

et al. 2016

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Calcium is considered an essential element for the metabolism of aquatic snail Biomphalaria glabrata (Say, 1818), intermediate host of Schistosoma mansoni Sambon, 1907 in Brazil, and represents a limiting factor to its distribution and adaptation to the environment. This study investigated the effect of different concentrations of exogenous CaCO 3 on the energetic metabolism of B. glabrata for better understanding the physiological interference of chemical elements dissolved in the environment with the physiology of this species. Sixty-day-old snails were distributed into six groups, five exposed to different concentrations of CaCO 3 (20, 40, 60, 80 and 100 mg/L) and a control group. The exposure to CaCO 3 was assessed over time, with analysis of 15 snails of each group in the following intervals: 1, 14, 21 or 30 days for hemolymph extraction. Concentrations of calcium and glucose in the hemolymph were determined by commercial kits, and organic acids were extracted using an ion exchange column and analyzed by high-performance liquid chromatography. Concentration of calcium in the hemolymph showed no significant difference (p>0.05) from the control group and between the concentrations tested. Concentration of glucose decreased (p<0.05) in the treatments of exposure to 20 and 40 mg/L and increased when exposed to 80 and 100 mg/L CaCO 3 compared to control and to other concentrations tested over 30 days. The organic acids pyruvate, oxaloacetate, citrate, succinate, fumarate, beta-hydroxybutyrate and lactate presented increased concentrations, while propionate and acetoacetate, decreased concentrations, when exposed to CaCO 3 compared to control. Considering the influence of different periods of exposure to CaCO 3 , on the 14 th day, there were stronger alterations in the metabolism of B. glabrata. In conclusion, exposure to CaCO 3 reduced the concentration of glucose, which is metabolized into pyruvate, the final product of glycolysis, and also influenced the energetic metabolism pathways, indicating an aerobic or partially anaerobic functioning.Keywords: snails, Krebs cycle, CaCO 3 exposure, energetic metabolism. (20, 40, 60, 80 e 100mg/L) e um controle. A exposição ao CaCO 3 foi avaliada em função do tempo, sendo retirados 15 moluscos de cada grupo nos seguintes intervalos: 1, 14, 21 ou 30 dias para extração da hemolinfa. As concentrações de cálcio e glicose na hemolinfa foram determinadas usando-se kits comercial e os ácidos orgânicos foram extraídos por meio da coluna de troca iônica e analisados através cromatografia líquida Alterações no metabolismo energético deBraz.

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High performance column liquid chromatographic analysis of selected carboxylic acids in Biomphalaria glabrata patently infected with Schistosoma mansoni

et al. 2007

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High-performance liquid chromatography (HPLC) was used to determine the effects of a patent Schistosoma mansoni infection on certain carboxylic acids in the digestive gland gonad complex (DGG) and hemolymph of Biomphalaria glabrata. An analysis of DGG samples was done using extraction with 50% Locke's solution, cleanup of the extract by anion exchange solid phase extraction (SPE), and ion exclusion HPLC with ultraviolet detection. Hemolymph was applied directly to the SPE column. Acetic, fumaric, malic, and pyruvic acids were detected, confirmed, and quantified at concentrations ranging from 12 to 280 ppm in the DGG and less than 124 to 8,000 microg/dl in the hemolymph. Infection with S. mansoni caused a significant reduction (Student's t test, P< 0.05) in the concentrations of acetic, fumaric, malic, and pyruvic acids in the DGG but not the hemolymph of B. glabrata compared to uninfected cohort snails. The significant reduction of certain carboxylic acids in the DGG of B. glabrata patently infected with S. mansoni suggests that these acids are utilized by the sporocysts and cercariae in the snail tissue, or that the infection stimulates reduced production or increased utilization by the snail tissue.

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Profile of organic acid concentrations in the digestive gland and hemolymph of Biomphalaria glabrata under estivation

Abstract: Using high performance liquid chromatography (HPLC) analysis it was possible to determine simultaneously the concentration of organic acids (pyruvate, lactate, succinate, fumarate, malate, acetate, propionate, acetoacetate, and ß-hydroxybutyrate)

Cited by 22 publications

References 21 publications

Carbohydrate metabolism alterations in Biomphalaria glabrata infected with Schistosoma mansoni and exposed to Euphorbia splendens var. hislopii latex

Carbohydrate metabolism alterations in Biomphalaria glabrata infected with Schistosoma mansoni and exposed to Euphorbia splendens var. hislopii latex

Changes in energetic metabolism of Biomphalaria glabrata (Mollusca, Planorbidae) in response to exogenous calcium

High performance column liquid chromatographic analysis of selected carboxylic acids in Biomphalaria glabrata patently infected with Schistosoma mansoni

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