Resolution of an Infection with Leishmania braziliensis Confers Complete Protection to a Subsequent Challenge with Leishmania major in BALB/c Mice

Lima, Hermenio C.; DeKrey, Gregory K.; Titus, Richard G.

doi:10.1590/s0074-02761999000100015

Cited by 15 publications

(19 citation statements)

References 30 publications

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“…This is a relevant finding since, epidemiologically, L. mexicana and L. chagasi coexist in wide areas of the world, causing very different forms of disease, i.e., visceral and cutaneous forms. In previous studies, cross-protection between Leishmania species has been mostly investigated by sequential infections with distinct species (1,17,26,27) or by infecting animal models with homologous or heterologous species after immunization with killed parasites (4). However, little is known about the specific antigens that may be involved.…”

Section: Discussionmentioning

confidence: 99%

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Cross-Protective Efficacy of a ProphylacticLeishmania donovaniDNA Vaccine against Visceral and Cutaneous Murine Leishmaniasis

et al. 2005

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Section: Discussionmentioning

confidence: 99%

“…Initial studies using sequential infections with distinct species have suggested complex crossprotection relationships (1,26,27). For example, mice recovered from a Leishmania major infection are resistant to a subsequent L. mexicana infection, but a primary infection with L. mexicana does not protect against a secondary infection with L. major (2).…”

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confidence: 99%

Cross-Protective Efficacy of a ProphylacticLeishmania donovaniDNA Vaccine against Visceral and Cutaneous Murine Leishmaniasis

et al. 2005

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“…Finally, heterologous protection induced by infection with one virulent species of Leishmania against infection with another species of Leishmania has been documented in animal model studies (37)(38)(39). In mice, experimental vaccination using dp72 protein isolated from L. donovani cross-protected against L. major infection (40) and immunization with exogenous Ags (LmSEAgs) of L. major cross-protected against L. donovani (41).…”

Section: Ldcen1mentioning

confidence: 99%

Intracellular Replication-Deficient Leishmania donovani Induces Long Lasting Protective Immunity against Visceral Leishmaniasis

Selvapandiyan

Dey

Nylén

et al. 2009

The Journal of Immunology

152

203

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No vaccine is currently available for visceral leishmaniasis (VL) caused by Leishmania donovani. This study addresses whether a live attenuated centrin gene-deleted L. donovani (LdCen1−/−) parasite can persist and be both safe and protective in animals. LdCen1−/− has a defect in amastigote replication both in vitro and ex vivo in human macrophages. Safety was shown by the lack of parasites in spleen and liver in susceptible BALB/c mice, immune compromised SCID mice, and human VL model hamsters 10 wk after infection. Mice immunized with LdCen1−/− showed early clearance of virulent parasite challenge not seen in mice immunized with heat killed parasites. Upon virulent challenge, the immunized mice displayed in the CD4+ T cell population a significant increase of single and multiple cytokine (IFN-γ, IL-2, and TNF) producing cells and IFN-γ/IL10 ratio. Immunized mice also showed increased IgG2a immunoglobulins and NO production in macrophages. These features indicated a protective Th1-type immune response. The Th1 response correlated with a significantly reduced parasite burden in the spleen and no parasites in the liver compared with naive mice 10 wk post challenge. Protection was observed, when challenged even after 16 wk post immunization, signifying a sustained immunity. Protection by immunization with attenuated parasites was also seen in hamsters. Immunization with LdCen1−/− also cross-protected mice against infection with L. braziliensis that causes mucocutaneous leishmaniasis. Results indicate that LdCen1−/− can be a safe and effective vaccine candidate against VL as well as mucocutaneous leishmaniasis causing parasites.

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“…braziliensis is the most common cause of local cutaneous leishmaniasis in South America. Over the years, only a few groups have started to investigate the immunological parameters that determine the course of L. braziliensis infections in mice (16,19,20,36,46,55). Cutaneous footpad or ear infections with L. braziliensis lead to transient, self-healing skin swelling in both BALB/c and C57BL/6 mice (16,19,20,37,55,62).…”

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confidence: 99%

“…Supernatants were collected at 16, 36, and 72 h for the detection of nitrite (NO 2 Ϫ ) using the Griess reaction (7). The percentage of infected macrophages and the number of intracellular parasites per infected cell, which were determined microscopically at 16,36, and 72 h of culture in duplicate wells after staining with Diff-Quick (Dade Behring, Marburg, Germany), were used to calculate the number of parasites per 100 macrophages in culture. All values given below are means Ϯ standard deviations and were based on evaluation of at least three microscopical fields with 100 macrophages each.…”

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Cytokines, Signaling Pathways, and Effector Molecules Required for the Control ofLeishmania(Viannia)braziliensisin Mice

et al. 2007

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Cutaneous leishmaniasis is caused by protozoan parasites of the genusParasites of the protozoan genus Leishmania are transmitted by sandflies to mammalian hosts, including humans, in which they elicit a spectrum of diseases that range from cutaneous to mucocutaneous and visceral leishmaniasis (48). One key factor that determines the clinical manifestation and course of infection is the parasite species. Thus, local cutaneous leishmaniasis is caused by Leishmania (subgenus Leishmania) major and L. (L.) tropica in Europe and the Near and Far East and by Leishmania (subgenus Viannia) braziliensis, L. (V.) guyanensis, and Leishmania (L.) mexicana in the Americas, whereas the prototypic species associated with visceral leishmaniasis are Leishmania (L.) donovani, Leishmania (L.) infantum, and (L.) chagasi (18,66)

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Resolution of an Infection with Leishmania braziliensis Confers Complete Protection to a Subsequent Challenge with Leishmania major in BALB/c Mice

Cited by 15 publications

References 30 publications

Cross-Protective Efficacy of a ProphylacticLeishmania donovaniDNA Vaccine against Visceral and Cutaneous Murine Leishmaniasis

Cross-Protective Efficacy of a ProphylacticLeishmania donovaniDNA Vaccine against Visceral and Cutaneous Murine Leishmaniasis

Intracellular Replication-Deficient Leishmania donovani Induces Long Lasting Protective Immunity against Visceral Leishmaniasis

Cytokines, Signaling Pathways, and Effector Molecules Required for the Control ofLeishmania(Viannia)braziliensisin Mice

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