Haemolymph and Fat Body Metallo-protease Associated with Enterobacter cloacae Infection in the Bloodsucking Insect, Rhodnius prolixus

Feder, Denise; Salles, Jussára M.; Garcia, Elói S.

doi:10.1590/s0074-02761998000600023

Cited by 5 publications

(4 citation statements)

References 10 publications

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“…Moreover, RNAi silencing of metalloproteases in Rhipicephalus microplus affects average egg weight and oviposition rate [50]. Two metalloproteases are expressed in the hemolymph of R. prolixus infected with Enterobacter cloacae [51] and Trypanosoma rangeli [52]. These proteases are expressed in the fat body and their release into the hemolymph upon infection suggests their role in R. prolixus defense against pathogens [53].…”

Section: Detailed Analysis Of the Differentially Expressed Proteins And Their Physiological Involvement In Blood Digestionmentioning

confidence: 99%

Early Post-Prandial Regulation of Protein Expression in the Midgut of Chagas Disease Vector Rhodnius prolixus Highlights New Potential Targets for Vector Control Strategy

et al. 2021

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Chagas disease is a vector-borne parasitic disease caused by the flagellated protozoan Trypanosoma cruzi and transmitted to humans by a large group of bloodsucking triatomine bugs. Triatomine insects, such as Rhodnius prolixus, ingest a huge amount of blood in a single meal. Their midgut represents an important interface for triatomine–trypanosome interactions. Furthermore, the development of parasites and their vectorial transmission are closely linked to the blood feeding and digestion; thus, an understanding of their physiology is essential for the development of new strategies to control triatomines. In this study, we used label-free quantitative proteomics to identify and analyze the early effect of blood feeding on protein expression in the midgut of Rhodnius prolixus. We both identified and quantified 124 proteins in the anterior midgut (AM) and 40 in the posterior midgut (PM), which vary significantly 6 h after feeding. The detailed analysis of these proteins revealed their predominant involvement in the primary function of hematophagy, including proteases, proteases inhibitors, amino acids metabolism, primary metabolites processing, and protein folding. Interestingly, our proteomics data show a potential role of the AM in protein digestion. Moreover, proteins related to detoxification processes and innate immunity, which are largely accepted to be triggered by blood ingestion, were mildly modulated. Surprisingly, one third of blood-regulated proteins in the AM have unknown function. This work contributes to the improvement of knowledge on the digestive physiology of triatomines in the early hours post-feeding. It provides key information for selecting new putative targets for the development of triatomine control tools and their potential role in the vector competence, which could be applied to other vector species.

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Section: Detailed Analysis Of the Differentially Expressed Proteins And Their Physiological Involvement In Blood Digestionmentioning

confidence: 99%

Early Post-Prandial Regulation of Protein Expression in the Midgut of Chagas Disease Vector Rhodnius prolixus Highlights New Potential Targets for Vector Control Strategy

et al. 2021

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“…Two metalloproteases are expressed in the haemolymph of R. prolixus infected with Enterobacter cloacae [ 121 ] or Trypanosoma rangeli [ 122 ]. The source of these proteases is the fat body and their release into the haemolymph upon infection suggests these enzymes may be involved in R. prolixus defence mechanisms.…”

Section: Proteases From Haematophagous Arthropod Vectorsmentioning

confidence: 99%

Proteases of haematophagous arthropod vectors are involved in blood-feeding, yolk formation and immunity - a review

et al. 2017

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Ticks, triatomines, mosquitoes and sand flies comprise a large number of haematophagous arthropods considered vectors of human infectious diseases. While consuming blood to obtain the nutrients necessary to carry on life functions, these insects can transmit pathogenic microorganisms to the vertebrate host. Among the molecules related to the blood-feeding habit, proteases play an essential role. In this review, we provide a panorama of proteases from arthropod vectors involved in haematophagy, in digestion, in egg development and in immunity. As these molecules act in central biological processes, proteases from haematophagous vectors of infectious diseases may influence vector competence to transmit pathogens to their prey, and thus could be valuable targets for vectorial control.Electronic supplementary materialThe online version of this article (doi:10.1186/s13071-017-2005-z) contains supplementary material, which is available to authorized users.

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“…It was described elsewhere that the inoculation of Enterobacter cloacae into R. prolixus haemocoel also induced two distinct patterns of protease ac-tivities in the haemolymph with the apparent molecular weights of 46 and 56 kDa (Feder et al 1998). Thus, the induction of these proteases in the haemolymph by the presence of parasite or bacterial infections is important.…”

Section: Discussionmentioning

confidence: 95%

Metalloproteases in Trypanosoma rangeli-infected Rhodnius prolixus

Feder¹,

Gomes²,

Garcia³

et al. 1999

Mem. Inst. Oswaldo Cruz

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Haemolymph and Fat Body Metallo-protease Associated with Enterobacter cloacae Infection in the Bloodsucking Insect, Rhodnius prolixus

Abstract: Analysis of zymograms with SDS

Cited by 5 publications

References 10 publications

Early Post-Prandial Regulation of Protein Expression in the Midgut of Chagas Disease Vector Rhodnius prolixus Highlights New Potential Targets for Vector Control Strategy

Early Post-Prandial Regulation of Protein Expression in the Midgut of Chagas Disease Vector Rhodnius prolixus Highlights New Potential Targets for Vector Control Strategy

Proteases of haematophagous arthropod vectors are involved in blood-feeding, yolk formation and immunity - a review

Metalloproteases in Trypanosoma rangeli-infected Rhodnius prolixus

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