Metalloproteases in Trypanosoma rangeli-infected Rhodnius prolixus

Feder, Denise; Gomes, Sérgio Bittencourt Varella; Garcia, Elói S.; Azambuja, Patrı́cia

doi:10.1590/s0074-02761999000600011

Cited by 15 publications

(4 citation statements)

References 20 publications

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“…The flagellates were then resuspended in sterile PBS (pH 7.2), and 4 µl of this suspension (5×10 4 cells parasites) were injected in each insect (adult insects seven days after moulting). The parasites were injected laterally into the thorax, between the second and third thoracic segments of the insects, using a 10-µl Hamilton syringe [84]. Control insects were injected with 4 µl sterile PBS.…”

Section: Methodsmentioning

confidence: 99%

Evidence That a Laminin-Like Insect Protein Mediates Early Events in the Interaction of a Phytoparasite with Its Vector's Salivary Gland

et al. 2012

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Phytomonas species are plant parasites of the family Trypanosomatidae, which are transmitted by phytophagous insects. Some Phytomonas species cause major agricultural damages. The hemipteran Oncopeltus fasciatus is natural and experimental host for several species of trypanosomatids, including Phytomonas spp. The invasion of the insect vectors' salivary glands is one of the most important events for the life cycle of Phytomonas species. In the present study, we show the binding of Phytomonas serpens at the external face of O. fasciatus salivary glands by means of scanning electron microscopy and the in vitro interaction of living parasites with total proteins from the salivary glands in ligand blotting assays. This binding occurs primarily through an interaction with a 130 kDa salivary gland protein. The mass spectrometry of the trypsin-digest of this protein matched 23% of human laminin-5 β3 chain precursor sequence by 16 digested peptides. A protein sequence search through the transcriptome of O. fasciatus embryo showed a partial sequence with 51% similarity to human laminin β3 subunit. Anti-human laminin-5 β3 chain polyclonal antibodies recognized the 130 kDa protein by immunoblotting. The association of parasites with the salivary glands was strongly inhibited by human laminin-5, by the purified 130 kDa insect protein, and by polyclonal antibodies raised against the human laminin-5 β3 chain. This is the first report demonstrating that a laminin-like molecule from the salivary gland of O. fasciatus acts as a receptor for Phytomonas binding. The results presented in this investigation are important findings that will support further studies that aim at developing new approaches to prevent the transmission of Phytomonas species from insects to plants and vice-versa.

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Section: Methodsmentioning

confidence: 99%

Evidence That a Laminin-Like Insect Protein Mediates Early Events in the Interaction of a Phytoparasite with Its Vector's Salivary Gland

et al. 2012

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“…Moreover, RNAi silencing of metalloproteases in Rhipicephalus microplus affects average egg weight and oviposition rate [50]. Two metalloproteases are expressed in the hemolymph of R. prolixus infected with Enterobacter cloacae [51] and Trypanosoma rangeli [52]. These proteases are expressed in the fat body and their release into the hemolymph upon infection suggests their role in R. prolixus defense against pathogens [53].…”

Section: Detailed Analysis Of the Differentially Expressed Proteins And Their Physiological Involvement In Blood Digestionmentioning

confidence: 99%

Early Post-Prandial Regulation of Protein Expression in the Midgut of Chagas Disease Vector Rhodnius prolixus Highlights New Potential Targets for Vector Control Strategy

et al. 2021

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Chagas disease is a vector-borne parasitic disease caused by the flagellated protozoan Trypanosoma cruzi and transmitted to humans by a large group of bloodsucking triatomine bugs. Triatomine insects, such as Rhodnius prolixus, ingest a huge amount of blood in a single meal. Their midgut represents an important interface for triatomine–trypanosome interactions. Furthermore, the development of parasites and their vectorial transmission are closely linked to the blood feeding and digestion; thus, an understanding of their physiology is essential for the development of new strategies to control triatomines. In this study, we used label-free quantitative proteomics to identify and analyze the early effect of blood feeding on protein expression in the midgut of Rhodnius prolixus. We both identified and quantified 124 proteins in the anterior midgut (AM) and 40 in the posterior midgut (PM), which vary significantly 6 h after feeding. The detailed analysis of these proteins revealed their predominant involvement in the primary function of hematophagy, including proteases, proteases inhibitors, amino acids metabolism, primary metabolites processing, and protein folding. Interestingly, our proteomics data show a potential role of the AM in protein digestion. Moreover, proteins related to detoxification processes and innate immunity, which are largely accepted to be triggered by blood ingestion, were mildly modulated. Surprisingly, one third of blood-regulated proteins in the AM have unknown function. This work contributes to the improvement of knowledge on the digestive physiology of triatomines in the early hours post-feeding. It provides key information for selecting new putative targets for the development of triatomine control tools and their potential role in the vector competence, which could be applied to other vector species.

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“…Two metalloproteases are expressed in the haemolymph of R. prolixus infected with Enterobacter cloacae [ 121 ] or Trypanosoma rangeli [ 122 ]. The source of these proteases is the fat body and their release into the haemolymph upon infection suggests these enzymes may be involved in R. prolixus defence mechanisms.…”

Section: Proteases From Haematophagous Arthropod Vectorsmentioning

confidence: 99%

Proteases of haematophagous arthropod vectors are involved in blood-feeding, yolk formation and immunity - a review

et al. 2017

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Ticks, triatomines, mosquitoes and sand flies comprise a large number of haematophagous arthropods considered vectors of human infectious diseases. While consuming blood to obtain the nutrients necessary to carry on life functions, these insects can transmit pathogenic microorganisms to the vertebrate host. Among the molecules related to the blood-feeding habit, proteases play an essential role. In this review, we provide a panorama of proteases from arthropod vectors involved in haematophagy, in digestion, in egg development and in immunity. As these molecules act in central biological processes, proteases from haematophagous vectors of infectious diseases may influence vector competence to transmit pathogens to their prey, and thus could be valuable targets for vectorial control.Electronic supplementary materialThe online version of this article (doi:10.1186/s13071-017-2005-z) contains supplementary material, which is available to authorized users.

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Metalloproteases in Trypanosoma rangeli-infected Rhodnius prolixus

Cited by 15 publications

References 20 publications

Evidence That a Laminin-Like Insect Protein Mediates Early Events in the Interaction of a Phytoparasite with Its Vector's Salivary Gland

Evidence That a Laminin-Like Insect Protein Mediates Early Events in the Interaction of a Phytoparasite with Its Vector's Salivary Gland

Early Post-Prandial Regulation of Protein Expression in the Midgut of Chagas Disease Vector Rhodnius prolixus Highlights New Potential Targets for Vector Control Strategy

Proteases of haematophagous arthropod vectors are involved in blood-feeding, yolk formation and immunity - a review

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