Molecular Epidemiologic Typing Systems of Bacterial Pathogens: Current Issues and Perpectives

Struelens, Marc

doi:10.1590/s0074-02761998000500004

Cited by 62 publications

(55 citation statements)

References 19 publications

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“…Unlike PFGE, isolates within the same epidemic clone always had the same ribotypes (Table 1). However, this can be explained by the insufficient discriminatory power of ribotyping due to high conservation in 16S and 23S RNA genes (51). For example, an isolate from the ice cream outbreak in Pennsylvania had the same ribotype (DUP-1038B) as ECI outbreak isolates, but this isolate did not belong to ECI as indicated by MVLST (Fig.…”

Section: Discussionmentioning

confidence: 99%

Multi-Virulence-Locus Sequence Typing Identifies Single Nucleotide Polymorphisms Which Differentiate Epidemic Clones and Outbreak Strains ofListeria monocytogenes

2007

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Section: Discussionmentioning

confidence: 99%

Multi-Virulence-Locus Sequence Typing Identifies Single Nucleotide Polymorphisms Which Differentiate Epidemic Clones and Outbreak Strains ofListeria monocytogenes

2007

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“…Molecular methods in general surpass phenotypic methods in typability, reproducibility and discriminative power. The method of choice highly depends on its performance of the above-mentioned criteria along with robustness, epidemiological concordance, operational ease, standardisation and inter-laboratory comparison capacity [39,60]. These methods have different benefits and should be chosen in view of the question addressed and the accuracy of the response which is expected.…”

Section: Arbitrarily Primed Pcr Multilocus Fingerprinting Methodsmentioning

confidence: 99%

Molecular tools for the characterisation of antibiotic-resistant bacteria

Aarts¹,

Sidi-Boumedine

Nesme

et al. 2001

Vet. Res.

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-This review will discuss a number of molecular tools which are currently used as well as some innovative approaches for the characterisation of antibiotic-resistant bacterial strains. Various methods involved in the detection and characterisation of genes and mutations associated with antibiotic resistance and that are used for strain typing as part of epidemiological studies, are described. Furthermore, a few examples are discussed in which the results of both gene and strain characterisation are combined to investigate the underlying mechanism of the spread of antibiotic resistance. Some of the available molecular techniques are heavily supported by the existence of databases on the Internet. These databases either contain a fast growing amount of sequence information or a large number of allelic or fingerprint profiles. The current progress in applied DNA technology and the ongoing projects on the elucidation of the whole genomic sequence of bacterial species have lead and will further lead to the development and application of sophisticated new strategies for the analysis of antibiotic resistant bacterial strains.antibiotic resistance / molecular detection / molecular typing / horizontal gene transfer / new approaches Résumé -Outils moléculaires pour la caractérisation de bactéries résistantes aux antibiotiques. Cette revue présente les outils moléculaires utilisés actuellement ainsi que des nouvelles approches pour la caractérisation de souches bactériennes résistantes aux antibiotiques. Différentes méthodes de détection et de caractérisation des gènes et des mutations impliqués dans la résistance aux antibiotiques, utilisées dans des études épidémiologiques, sont décrites. Quelques exemples sont présentés où sont associées les caractérisations du gène et de la souche bactérienne dans l'étude des mécanismes de diffusion de résistances aux antibiotiques. Certaines techniques moléculaires font

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“…The Hunter-Gaston diversity index (HGDI) was calculated to evaluate the discriminatory power of all ERIC-PCR tests (Hunter and Gaston, 1988). Typeability was evaluated from the proportion of isolates that were scored in the ERIC-PCR assays and assigned a type (Struelens, 1998).…”

Section: Genotype Analysismentioning

confidence: 99%

Short Communication Molecular characterization of Corynebacterium pseudotuberculosis isolated from goats using ERIC-PCR

Dorneles

Santana²,

Andrade³

et al. 2012

Genet. Mol. Res.

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Molecular Epidemiologic Typing Systems of Bacterial Pathogens: Current Issues and Perpectives

Cited by 62 publications

References 19 publications

Multi-Virulence-Locus Sequence Typing Identifies Single Nucleotide Polymorphisms Which Differentiate Epidemic Clones and Outbreak Strains ofListeria monocytogenes

Multi-Virulence-Locus Sequence Typing Identifies Single Nucleotide Polymorphisms Which Differentiate Epidemic Clones and Outbreak Strains ofListeria monocytogenes

Molecular tools for the characterisation of antibiotic-resistant bacteria

Short Communication Molecular characterization of Corynebacterium pseudotuberculosis isolated from goats using ERIC-PCR

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