Immunodiagnosis of Trypanosoma cruzi (Chagas' Disease) Infection in Naturally Infected Dogs

Lauricella, Marta A.; Castañera, Mónica B.; Gürtler, Ricardo E.; Segura, Elsa L.

doi:10.1590/s0074-02761998000400016

Cited by 37 publications

(53 citation statements)

References 12 publications

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“…These included groups of (A) healthy dogs (N = 80) and (B) ill dogs (N = 41), affected by infectious and other diseases including distemper, brucellosis, bacterial infections, and noninfectious pathologies as neoplasm, anemias, renal failure, urolithiasis, Cushing syndrome, heart disease, vestibular syndrome, prolapsed organs, and inflammatory diseases; group 2: Trypanosoma cruzi-infected dogs (N = 108) from rural areas of Santiago del Estero and Chaco, both endemic Argentinean provinces for Chagas disease, which were diagnosed using two or three serological tests. 19,20 Group 3 corresponds to T. cruzi-noninfected dogs living in Chaco (N = 71) in the same area as those dogs mentioned in group 2.…”

Section: Methodsmentioning

confidence: 99%

“…Trypanosoma cruzi serology in dogs was performed by using T. cruzi antigens in IFA, ELISA, and indirect hemaglutination assay to evaluate the infection status of dogs. 19 VL serology in dogs was performed using rK39 ICT (Kalazar Detect Canine Rapid Test, lot no. JM 1062 and PM 1020 from Inbios International, Inc., Seattle, WA) with manufacturer's instructions to process 151/167 case sera samples and 296/300 VL noninfected control dog sera.…”

Section: Methodsmentioning

confidence: 99%

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An rK28-Based Immunoenzymatic Assay for the Diagnosis of Canine Visceral Leishmaniasis in Latin America

Lauricella

Maidana

Frias

et al. 2016

The American Journal of Tropical Medicine and Hygiene

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Abstract. Direct observation of Leishmania parasites in tissue aspirates has shown low sensitivity for the detection of canine visceral leishmaniasis (VL). Therefore in the last quarter century immunoenzymatic tests have been developed to improve diagnosis. The purpose of this study was to develop a fast recombinant K28 antigen, naked-eye qualitative enzyme-linked immunosorbent assay (VL Ql-ELISA) and a quantitative version (VL Qt-ELISA), and to display it in a kit format, whose cutoff value (0.156) was selected as the most adequate one to differentiate reactive from nonreactive samples. Considering 167 cases and 300 controls, sensitivity was 91% for both assays and specificity was 100% and 98.7% in Ql-ELISA and Qt-ELISA, respectively. Positive predictive values were 100% and 97.4% for Ql-ELISA and Qt-ELISA, respectively, and negative predictive values were 95.2% for both ELISAs. Reagent stability, reliability studies, including periodic repetitions and retest of samples, cutoff selection, and comparison of rK28 ELISAs with rK39 immunochromatographic test, were the international criteria that supported the quality in both kits. The performance of both ELISA kits in this work confirmed their validity and emphasized their usefulness for low-to-medium complexity laboratories.

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

An rK28-Based Immunoenzymatic Assay for the Diagnosis of Canine Visceral Leishmaniasis in Latin America

Lauricella

Maidana

Frias

et al. 2016

The American Journal of Tropical Medicine and Hygiene

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“…Detection of antibodies to T. cruzi by the indirect haemagglutination test (IHA; Polychaco, Argentina), enzyme-linked immunosorbent assay (ELISA), and the immunofluorescent antibody tests (IFAT, in a subsample) was as described by Lauricella et al (1998). Seroreactivity for T. cruzi of dog sera was demonstrated by ELISA readings of 0·2 or greater and IFAT and IHA titres of 1 : 16 or greater; sensitivity was estimated as 94%, 94% and 68%, whereas specificity was 96·2%, 100% and 100%, respectively.…”

Section: Sero-parasitological Surveysmentioning

confidence: 99%

Domestic dogs and cats as sources ofTrypanosoma cruziinfection in rural northwestern Argentina

et al. 2006

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SUMMARYThe reservoir capacity of domestic cats and dogs for Trypanosoma cruzi infection and the hostfeeding patterns of domestic Triatoma infestans were assessed longitudinally in 2 infested rural villages in north-western Argentina. A total of 86 dogs and 38 cats was repeatedly examined for T. cruzi infection by serology and/or xenodiagnosis. The composite prevalence of infection in dogs (60%), but not in cats, increased significantly with age and with the domiciliary density of infected T. infestans. Dogs and cats had similarly high forces of infection, prevalence of infectious hosts (41-42%), and infectiousness to bugs at a wide range of infected bug densities. The infectiousness to bugs of seropositive dogs declined significantly with increasing dog age and was highly aggregated. Individual dog infectiousness to bugs was significantly autocorrelated over time. Domestic T. infestans fed on dogs showed higher infection prevalence (49%) than those fed on cats (39%), humans (38%) or chickens (29%) among 1085 bugs examined. The basic reproduction number of T. cruzi in dogs was at least 8·2. Both cats and dogs are epidemiologically important sources of infection for bugs and householders, dogs nearly 3 times more than cats.

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“…Sera from dogs were tested for antibodies to T. cruzi by indirect hemagglutination assay (IHA; Polychaco, Buenos Aires, Argentina), indirect immunofluorescence test (IFAT) and enzymelinked immunosorbent assay (ELISA) using standardized procedures and criteria reported elsewhere (Lauricella et al, 1998). Sera from cat were tested for antibodies to T. cruzi by IHA (Polychaco, Buenos Aires, Argentina), IFAT (total antigamma LID, Laboratorio Inmunodiagnóstico, Buenos Aires, Argentina) and ELISA (goat anti-cat IgG HRP, Santa Cruz Biotechnology, Santa Cruz, California).…”

Section: Serodiagnosis and Xenodiagnosismentioning

confidence: 99%

“…Sera from cat were tested for antibodies to T. cruzi by IHA (Polychaco, Buenos Aires, Argentina), IFAT (total antigamma LID, Laboratorio Inmunodiagnóstico, Buenos Aires, Argentina) and ELISA (goat anti-cat IgG HRP, Santa Cruz Biotechnology, Santa Cruz, California). Titers ≥1:16 (IHA and IFAT) or an optical absorbance ≥0.2 (ELISA) were used as cutoff values for dogs and cats (Lauricella et al, 1998). Xenodiagnosis was performed using 20 laboratory-reared, third-or fourth-instar nymphs of T. infestans per animal for 25 minutes .…”

Section: Serodiagnosis and Xenodiagnosismentioning

confidence: 99%

Impact of community-based vector control on house infestation and Trypanosoma cruzi infection in Triatoma infestans, dogs and cats in the Argentine Chaco

et al. 2007

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The relative impact of two community-based vector control strategies on house infestation by Triatoma infestans and Trypanosoma cruzi infection in bugs, domestic dogs and cats was assessed in two neighboring rural areas comprising 40 small villages and 323 houses in one of the regions most endemic for Chagas disease in northern Argentina. The prevalence and abundance of domestic infestation were 1.5-and 6.5-fold higher, respectively, in the area under pulsed, non-supervised control actions operating under the guidelines of the National Vector Control Program (NCVP) than in the area under sustained, supervised surveillance carried out jointly by the UBA research team and NCVP. The prevalence of infestation and infection varied widely among village groups within each area. In the pulsed control area, the prevalence of infection in bugs, dogs and cats was two-to three-fold higher than in the area under sustained surveillance, most of the infected animals qualified as autochthonous cases, and evidence of recent transmission was observed. Infection was highly aggregated at the household level and fell close to the 80/20 rule. Using multiple logistic regression analysis clustered by household, infection in dogs was associated positively and significantly with variables reflecting local exposure to infected T. infestans, thus demonstrating weak performance of the vector surveillance system. For high-risk areas in the Gran Chaco region, interruption of vectormediated domestic transmission of T. cruzi requires residual insecticide spraying that is more intense, of a higher quality and sustained in time, combined with community participation and environmental management measures.

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Immunodiagnosis of Trypanosoma cruzi (Chagas' Disease) Infection in Naturally Infected Dogs

Cited by 37 publications

References 12 publications

An rK28-Based Immunoenzymatic Assay for the Diagnosis of Canine Visceral Leishmaniasis in Latin America

An rK28-Based Immunoenzymatic Assay for the Diagnosis of Canine Visceral Leishmaniasis in Latin America

Domestic dogs and cats as sources ofTrypanosoma cruziinfection in rural northwestern Argentina

Impact of community-based vector control on house infestation and Trypanosoma cruzi infection in Triatoma infestans, dogs and cats in the Argentine Chaco

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